Prevalence and antibiotic resistance pattern of Acinetobacter spp. infections in Shahrekord medical centers

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Tohid Piri Gharaghie ¹ , Abbas Doosti ² , Seyed Abbas Mirzaei ³

1 - Department of Biology, Faculty of Basic Sciences, Islamic Azad University of Shahrekord Branch, Shahrekord, Iran.
2 - Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
3 - Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.

Received: 2021-05-20 Accepted : 2021-08-23 Published : 2021-11-22

Keywords: Antibiotic susceptibility, Primer Design, Acinetobacter spp,

Abstract :

the study estimated the prevalence and pattern of antimicrobial resistance of Acinetobacter spp. isolate from different clinical specimens. Primer design for Acinetobacter spp. was performed with Bioinformatics software Primer Express and Gene Runner. Primer authentication was performed by BLASTn online tool and Sequence Match program. Acinetobacter isolates were identified from different clinical specimens by standard biochemical methods and PCR tests. Antimicrobial susceptibility tests and biofilm detection were performed for isolates identified by standard disk diffusion method according to CLSI‌ protocol and Microtiter plate. Acinetobacter spp. were identified by designed primer with Query Cover and Per. Ident 100%. From 60 clinical samples, 243 bacterial isolates were obtained. 131 isolates (53.9%) were related to gram-positive bacteria and 112 isolates (46.09%) were gram-negative bacteria, of which 43 isolates (17.69%) Were identified as Acinetobacter spp. According to the PCR test, 31 strains (77.5%) were identified as Acinetobacter baumannii, 7 strains (17.5%) as Acinetobacter lwoffii, 2 strains (5%) as Acinetobacter junii. Antibiotic susceptibility study showed that all isolated strains were MDR and 87.5% of isolates were XDR. However, only 12.5% of the isolates were sensitive to carbapenems, All Acinetobacter isolates were biofilm positive and were identified as strong biofilms with a total mean of 0.213. According to the study, it is clear that infection with Acinetobacter can lead to significant challenges in the country's health care system in the future. To this end, finding solutions to prevent infection of this bacterial genus, especially Acinetobacter baumannii, is very important and necessary.