Study of the effect of oxidative stress on Nrf-2 cell signaling pathway in mesenchymal stem cells (MSCs)

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

parisa bostani ¹ , elnaz khojaste ² , changiz ahmadizadeh ³

1 - Departement of biology,Bonab Branch,Islamic Azad University, Bonab ,Iran
2 - Departement of biology,Ahar Branch,Islamic Azad University, Ahar ,Iran
3 - Microbiology, Department of Microbiology, Faculty of Basic Science,Islamic Azad University, Ahar Branch, Ahar,Iran

Received: 2020-06-29 Accepted : 2020-12-25 Published : 2020-11-21

Keywords: Oxidative stress, Stem Cells Mesenchymal, Nrf2,

Abstract :

The study of mesenchymal stem cells showed that induction of autophagy in MSCs increased sensitivity to oxidative stress Maharatophagy induced resistance of MSCs to oxidative stress and increased survival of MSCs compared to control cells. The aim of this study was to investigate the effect of oxidative stress on the Nrf2 cell signaling pathway in mesenchymal stem cells. The present study was conducted at the Pharmaceutical Micro-Technology Research Center of Tabriz Medical Sciences in 2017. In this experimental study, mesenchymal stem cells were cultured in DMEM-F12 medium and after trypsinization, re-culture was performed and the fourth cell passage was selected. Then it was examined using MTT assay method. DNA extraction was performed from treated cells and DNA Ladder assay was performed. After cDNA preparation, the expression of Nrf2 genes in mesenchymal stem cells was measured by real-time PCR. Data analysis was performed using SPSS software. done. The results showed that oxidative stress could activate the Nrf2-Nngoi cell signaling pathway and that stem cells could inhibit stress through this pathway. Therefore, it is necessary to strengthen this pathway through dietary antioxidants and supplements to increase the effectiveness of cell therapy.