Investigation of diversity and abundance of bacterial populations of Qeynarjeh hot spring: Identification of microbial ecosystem of the hottest hot spring in Iran by culture independent methods and based on next generation sequencing

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Reza Azarbaijani ¹ , laleh parsa yeganeh ²

1 - Molecular bank, Iranian Biological Resource Center (IBRC), ACECR, Tehran, Iran
2 - Molecular bank, Iranian Biological Resource Center (IBRC), ACECR, Tehran, Iran

Received: 2019-12-22 Accepted : 2020-01-28 Published : 2020-11-21

Keywords: : Extreme ecosystem, Qeynarjeh hot spring, metagenomics, microbiome,

Abstract :

Hot spring extreme ecosystems are important biological resources for identifying the genetic stocks of thermophilic microorganisms and their biological characteristics in terms of tolerance and survival in high temperatures in order to use of their potential in various industries and biotechnology. Although there have been many studies on the microbiome of these biomass in recent years in the world, so far few studies have been done on such indigenous extreme environments of Iran. Iran has a variety of geothermal and volcanic areas which contained numerous unique springs with antimicrobial and antifungal, anti-rheumatism and other muscle aches properties. In this study, the identification of composition of different taxa of the bacterial community of Qeynarjeh hot spring as one of the hottest chloride springs in the world with the temperature of 86◦ C was performed by independent cultivation methods using next generation sequencing techniques. For quantitative profiling of the relevant bacterial community or so-called metagenomics analysis, 16s rRNA gene libraries were constructed from three functional regions of taxonomic classification V1 to V3 with barcoded primers. By bioinformatics analysis, 200 different taxa belonging to different bacterial families and genera were identified with MG-RAST and SILVAngs systems. The dominant taxa with the most abundant, are respectively: Pediococcus acidilactici، uncultured (unclassified) bacterium، Lactobacillus rhamnosus، Thermus scotoductus، Thermus sp. HR13 و Thermus sp. BXR.