Investigation of BAD Gene Expression Following Chloriazepoxide Effect on pregnancy In the hippocampus of mouse infants

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Amin  Dinarvand ¹ (department of genetics,Islamic Azad University,Marvdasht branch,Marvdasht,Iran)
Mehrdad  Hashemi ² (Faculty of Advanced Scienced and technology, Islamic Azad University, Tehran Medical Sciences, Tehran, Iran)
Rasool  Dinarvand ³ (Department of Pharmacy, Faculty of Pharmacy, Tehran medical sciences University,Tehran,Iran)
Shabnam  Movaseghi ⁴ (Department of Anatomy,Faculty of medicine, Tehran medical sciences ,Islamic Azad University,Tehran,Iran)
Mojtaba  Jafarinia ⁵ (Department of Genetics,Islamic Azad University,Marvdasht branch,Marvdasht,Iran)

Received: 2019-02-03 Accepted : 2019-03-09 Published : 2019-05-22

Keywords: Gene expression, Hippocampus, Rat, Chlorodiazepoxide, bad gene,

Abstract :

Background: Chlorodiazepoxide is an anxiolytic agent commonly used by young people and pregnant women to reduce anxiety and control preeclampsia and eclampsia. Some studies have shown that this medication disrupts the functioning of the cholinergic system. Due to the increased cellular damage of bad gene expression, the effect of cholorodiazepoxide use during pregnancy on bad gene expression in the hippocampus of neonatal rats was investigated. Methods: In this study, 9 female Wistar rats were pregnant and randomly divided into three groups: control, experimental (intraperitoneal injection of chlordiazoxoxide 10 mg / kg for 21 days) and carriers (saline). Two weeks after the birth, the brain of the neonates was removed from the skull and the expression of the propaoptotic gene of Bad was investigated. Results: The level of gene expression was analyzed by Rest software and a significant level of P Conclusion: The results of this study indicated that the administration of chlorodiazepoxide during pregnancy can cause neuronal damage in the hippocampus of Wistar rats.