-The effect of various growth regulator compounds on gynogenesis of Allium ampeloprasum Taree group in vitro

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Mohammad Javad Shakouri ¹ , Mohammad Reza Hassandokht ² , Sepideh Kalateh Jari ³ , Kambiz Larijani ⁴ , Faezeh Ghanati ⁵

1 - Islamic Azad University, Shahriar Branch
2 - Department of Horticulture, Agriculture and Natural Resources Faculty, Tehran University, Karaj, Iran
3 - Department of Horticulture, Science and Research Branch, Islamic Azad University, Tehran, Iran
4 - Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran
5 - Department of Plant Biology, Faculty of Biological Science, Tarbiat Modares University (TMU), POB 14115-154

Received: 2018-09-01 Accepted : 2018-10-24 Published : 2019-05-22

Keywords: regeneration, Breeding, Embryogenesis, Flower Cultivation,

Abstract :

Persian leek (Allium ampeloprasum) has a special place in Iraninan's food chain. Many accessions by different characteristics have been cultivated and adapted in different regions of Iran. In order to investigate the breeding of this vegetable, an experiment was conducted in a randomized complete block design in three replications. For this purpose, 7 accessions of Persian leeks were selected. Various growth regulator componds2, 4 D at 0, 2 and 4 mg/lit and BA with 0, 2 and 4 mg/l were used in culture medium. The treatments were done on unopened flowers' umbrella and embryogenesis, regeneration and callus percentages of micro samples and the number of haploid plants were measured. The results indicated that Shadegan accessions in culture medium including 4 mg/l BA and 2 mg/l D-4,2 had the highest Percentage of embryogenesis (12.81 %) and regeneration (12.6%). The highest percentage of callus (0.51%) and lowest percentage of callus (0.16%) have been observed in arak and shadegan accession, respectively. Out of 42525 flowers cultivated, 1001 embryo (2.35%), 972 regenerate (97.1%) and 946 plants (94.5%) have survived. Eventually, seven haploid plants were observed. The highest number of haploid was observed in culture medium with 4 mg /liters BA and 2 mg/liters 2.4.D. in Guilan accession.