Evaluation of cytotoxic effect of Ruta graveolens L. Hydro alcoholic extract in human cervical cancer cells (HeLa)

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

seyed milad mousavi jazayeri ¹ , elahe ebrahimi ² , Masoud parsania ³

1 - Department of microbiology, Islamic Azad University, Science and Research Branch, Gilan, Iran.
2 - Department of microbiology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
3 - . Department of Microbiology, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.

Received: 2018-11-02 Accepted : 2019-03-17 Published : 2019-02-20

Keywords: HeLa cells, Cytotoxic effect, Ruta graveolens L. extract,

Abstract :

Abstract Introduction Cervical cancer is one of the most common cancers in women. In some cases, the lack of appropriate response to treatment with chemical drugs and the side effects of using synthetic drugs show necessary to achieve a natural drug that has minimal side effects. This study was evaluated effects of Hydroalcoholic extracts Ruta graveolens L. against of HeLa cell. Material and Methods HeLa cells were cultivated in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum. Preparation of extract were done by maceration technique. This study were assessed the effects of different concentrations (250, 500, 750, 1000 and 1500 μg/ml) of Ruta graveolens L. on the proliferation of HeLa cells at 24, 48 and 72 hours after treatment of cells with extract and medium containing 1% serum by Trypan blue and MTT methods. Finding The cytotoxic concentration 50% (CC50) of the extract was determined on HeLa cell 1500 ug/ml. The results showed that concentrations of 1000 ug/ml of Ruta graveolens L. extract, after 72 hours showed a significant decrease in the proliferation of HeLa cells compared with control. Conclusion: The results of this study showed Ruta graveolens L. extract can inhibit the proliferation of HeLa cells. Keyword: Cytotoxic effect, Ruta graveolens L. extract, HeLa cells.