Indirect regeneration of endangered Dracocephalum kotschyi Boiss

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

parisa jonubi ¹ , Ahmad Majd ² , mitra zamani nasrabadi ^{3 *}

1 - KHarazmi Tehran
2 - Kharazmi Tehran
3 - Department of Plant Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran

Received: 2017-10-15 Accepted : 2018-05-06 Published : 2018-02-20

Keywords: shoot regeneration, Keywords: callus induction, Daracocephalum Kotschyi Boiss, micro propagation,

Abstract :

Daracocephalum Kotschyi Boiss is a pharmaceutical and aromatic species of Lamiaceae which could be found in the northern and the north-eastern highlands of Iran. D. Kotschyi is an endangered species due to specific circumstances of habitat and human indiscriminated harvest. In vitro culture of different explants from sterile seedlings showed that all of media used for hypocotyl just produced callus without any shoot regeneration. The best callus production obtained from 14 day old - cotyledonary leaves cultured on MS medium containing 0.5 mg l-1 BA and 0.5 mg l-1 NAA. These calli were subcultured to MS medium containing 2 mg l-1 BA and 0.2 mg l-1 NAA that showed 33.3% shoot regeneration. In order to indirect regeneration using sequential reculturing, cotyledonary leaves on MS medium containing 1 mg l-1 BA and 1 mg l-1 NAA that 53.3% shoot regeneration was obtained. 75% from regenerated plants that were subcultured on root induction medium supplemented by 2 mg l-1 NAA could produce the strong roots. These plants were transferred to vermiculite and soil.They were adapted to greenhouse condition.

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