The effect of umbilical cord stromal cells and chicory extract on the repair process of amputated sciatic nerve in male Wistar rats (Behavioral and electrophysiological study).

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

amirhosin fazlali ¹ , Hayati Rodbari nasim ² , gholamreza Kaka ³ , kazem parivar ⁴ , homayoon sadrai ⁵

1 - PhD student of Cell Biology - Islamic Azad University - Science and Research Branch-Tehran, Iran
2 - Department of Cell Biology, Faculty of Basic Sciences, Islamic Azad University,Research Branch, Tehran, Iran.
3 - Anatomy, Baqiyatallah University of Medical Sciences - Tehran, Iran
4 - Department of Cellular Developmental Biology, Faculty of Basic Sciences, Islamic Azad University - Science and Research Branch - Tehran, Iran
5 - Baqiyatallah University of Medical Sciences - Tehran, Iran

Received: 2021-07-02 Accepted : 2022-01-18 Published : 2022-11-22

Keywords: "rat", "Cord blood cells", "Chicory extract", "Sciatic nerve",

Abstract :

Background and purpose: In this study, the therapeutic effect of umbilical cord blood cells with chicory extract on repair of severed sciatic nerve in adult male Wistar rats was evaluated by behavioral, electrophysiological study.Materials and Methods: After sciatic nerve amputation, adult male rats were randomly divided into 4 groups of 7.Healthy rats, rats with sciatica without treatment intervention, rats with umbilical cord blood cells treated with chicory extract at the site of amputation, rats with umbilical cord blood cells injected at the site of injury.The rate of recovery was assessed by sensory motor activity of the sciatic nerve, electrophysiological studies.Results: Sciatic nerve motor evaluation, no control group returned to normal in the eighth week, cell therapy group was restored with chicory extract in the eighth week. The level of AMP in the eighth week after the restoration of the cell therapy group with a gentle slope indicates the recovery process of the cell therapy group.Counting the number of nerve fibers at an area of 1000 μm, the number of nerve fibers in the cell therapy groups increased in the eighth week after repair, compared with the control group and the PLGA membrane group. By the end of the eighth week, the sciatic nerve index (Hot Plate test), the healing process of the cell therapy group with chicory extract was more evident to other groups.Conclusion: Cord blood cell transplantation repairs sciatic nerve and chicory extract along with umbilical cord blood cells accelerates sciatic nerve repair.