Synergistic effects of central glutamatergic and histaminergic systems on food intake in neonatal chickens: The role of NMDA receptors

Subject Areas : Journal of Comparative Pathobiology

مینا Mobarhan ¹ , مرتضی Zendehdel ² , بیتا Vazir ³ , احمد Asghari ⁴

1 - Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
3 - Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
4 - Department of Clinical Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Received: 2022-09-27 Accepted : 2022-12-08 Published : 2022-12-22

Keywords: Food Intake, Histamine, Glutamate, chicken,

Abstract :

Appetite modulation is a set of complex physiological mechanisms affecting different central nervous system areas. Glutamate and histamine play an important role in the central control of food intake in birds and reduce food intake in birds. The present study was conducted to investigate the synergistic effects of central glutamatergic and histaminergic systems on feeding behavior in neonatal chicks. 36 chickens were randomly divided into three experimental groups. Each experiment included a control group and 3 treatment groups (12 chickens in each group). In all experiments, after 3 hours of food deprivation (FD3), chicks received either control diluent or drug solution by intracerebroventricular (ICV) injection. Then the birds had ad libitum access to the ‎food and fresh water, and then cumulative food intake (gr) was measured based on the ‎percentage of the body (%BW) ‎. In the first experiment, glutamate (75 nmol), histamine (75 nmol), and histamine + glutamate was injected. In the second experiment, chlorpheniramine (H1 receptor antagonist, 300 nmol), MK-801 (NMDA glutamate receptor antagonist, 15 nmol), and chlorpheniramine + MK-801 was injected. In the third experiment, MK-801, α-FMH (histamine synthesis inhibitor, 250 nmol), and α-FMH + MK-801 was injected. The results showed that the injection of sub-effective doses of histamine with a dose of 75 nmol (sub-effective dose) and glutamate with a dose of 75 nmol (sub-effective dose) did not affect food intake (p > 0.05). However, co-injection of glutamate and histamine decreased food intake (p < 0.05). Injection of chlorpheniramine with a dose of 300 and MK-801 with a dose of 15 had no effect on food intake (p > 0.05). However, co-injection of chlorpheniramine and MK-801 increased food intake (p < 0.05). Injection of α-FMH with a dose of 250 and MK-801 with a dose of 15 did not influence food intake (p > 0.05). However, co-injection of α-FMHs and MK-801 increased food intake (p < 0.05). According to the results, there is probably a synergistic effect between histaminergic and glutamatergic systems in the control of food intake in neonatal chicks.

References:

1.Zendehdel M, Ghashghayi E, Hassanpour S, Baghbanzadeh A, Jonaidi H. Interaction between opioidergic and dopaminergic systems on food intake in neonatal layer type chicken. International Journal of Peptide Research and Therapeutics. 2016;22(1):83-92.

2. Zendehdel M, Sardari F, Hassanpour S, Rahnema M, Adeli A, Ghashghayi E. Serotonin-induced hypophagia is mediated via α2 and β2 adrenergic receptors in neonatal layer-type chickens. British Poultry Science. 2017;58(3):298-304.

3. Rahmani B, Ghashghayi E, Zendehdel M, Khodadadi M, Hamidi B. The Crosstalk Between Brain Mediators Regulating Food Intake Behavior in Birds: A Review. International Journal of Peptide Research and Therapeutics. 2021;27(4):2349-70.

4. Michaelis EK. Molecular biology of glutamate receptors in the central nervous system and their role in excitotoxicity, oxidative stress and aging. Progress in neurobiology. 1998;54(4):369-415.

5. Li P, Zhuo M. Silent glutamatergic synapses and nociception in mammalian spinal cord. Nature. 1998;393(6686):695.

6. Ciranna á. Serotonin as a modulator of glutamate-and GABA-mediated neurotransmission: implications in physiological functions and in pathology. Current neuropharmacology. 2006;4(2):101-14.

7. Mortezaei SS, Zendehdel M, Babapour V, Hasani K. The role of glutamatergic and GABAergic systems on serotonin-induced feeding behavior in chicken. Veterinary research communications. 2013;37(4):303-10.

8. Taati M, Babapour V, Kheradmand A, Tarrahi M. The role of central endogenous histamine and H1, H2 and H3 receptors on food intake in broiler chickens. Iranian Journal of Veterinary Research. 2009;10(1):54-60.

9. Qi W, Ding D, Salvi RJ. Cytotoxic effects of dimethyl sulphoxide (DMSO) on cochlear organotypic cultures. Hearing research. 2008;236(1-2):52-60.

10. Vazir B, Zendehdel M, Asghari A. Interaction of central glutamatergic and histaminergic systems on food intake regulation in layer chickens. Archives of Razi Institute. 2021;76(3):537.

11. Ghashghayi E, Zendehdel M, Khodadadi M, Rahmani B. Central dopaminergic, serotoninergic, as well as GABAergic systems mediate NMU-induced hypophagia in newborn chicken. International Journal of Neuroscience. 2022:1-11.

12. van Tienhoven At, Juhasz L. The chicken telencephalon, diencephalon and mesencephalon in stereotaxic coordinates. Journal of Comparative Neurology. 1962;118(2):185-97.

13. Saito E-S, Kaiya H, Tachibana T, Tomonaga S, Denbow DM, Kangawa K, et al. Inhibitory effect of ghrelin on food intake is mediated by the corticotropin-releasing factor system in neonatal chicks. Regulatory peptides. 2005;125(1-3):201-8.

14. Bradbury MJ, Campbell U, Giracello D, Chapman D, King C, Tehrani L, et al. Metabotropic glutamate receptor mGlu5 is a mediator of appetite and energy balance in rats and mice. Journal of Pharmacology and Experimental Therapeutics. 2005;313(1):395-402.

15. Barbato G. Genetic control of food intake in chickens. The Journal of nutrition. 1994;124(suppl_8):1341S-8S.

16. Baghbanzadeh A, Babapour V. CNS glutamatergic control of food intake in domestic fowl. Appetite. 2001;37:267.

17. Rafiei M, Taati M, Alavi S, Nayebzadeh H, Zendehdel M. Effects of intracerebroventricular injection of histamine and H1, H2 receptor antagonists on electrocardiographic parameters in broiler chickens. 2011.

18. Morimoto T, Yamamoto Y, Yamatodani A. Brain histamine and feeding behavior. Behavioural Brain Research. 2001;124(2):145-50.

19. Taati M, Babapour V, Kheradmand A, TARAHI M. The role of central endogenous histamine and H1, H2 and H3 receptors on food intake in broiler chickens. 2009.

20. Panula P, Nuutinen S. The histaminergic network in the brain: basic organization and role in disease. Nature Reviews Neuroscience. 2013;14(7):472.

21. Fell MJ, Flik G, Dijkman U, Folgering JH, Perry KW, Johnson BJ, et al. Glutamatergic regulation of brain histamine neurons: In vivo microdialysis and electrophysiology studies in the rat. Neuropharmacology. 2015;99:1-8.

22. Schoepp DD. Unveiling the functions of presynaptic metabotropic glutamate receptors in the central nervous system. Journal of Pharmacology and Experimental Therapeutics. 2001;299(1):12-20.