Decellularization of ostrich plantar ligament

Subject Areas : Journal of Comparative Pathobiology

روزبه Moridpour ¹ , حمیدرضا Fattahian ² , پژمان Mortazavi ³

1 - Department of Clinical Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Clinical Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.
3 - Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Received: 2022-10-01 Accepted : 2023-02-11 Published : 2023-02-11

Keywords: Decellularization, Ligamentum plantar, Ostrich,

Abstract :

The use of tissue is very common to return organ function nowadays. Tissue engineering and novel preparation techniques in therapy are a main role. In present research use of chemical and physical methods was tried to prepare tissue from connective tissue of plantar structure of ostrich. Five pair of ostrich is feet separated of tarsometatarsal joint were sent to laboratory. Microscopic study and biomechanical evaluation were performed. De-celluraization of the tissue was done with sodium dodecyl sulfate and vacuum. Finally, light and electron microscope studies were carried out. Biomechanical evaluation was done as well. Ligamentum plantar composed of fibrocartilage tissue that decellularization process last as percent of cells and their nucleus content. There was not significant difference between prepared tissue and original tissue in tension and compression parameters. SDS with vacuum decellularized till as percent with connective tissue unchanged with minimal antigenicity feature as xenograft. The tissue could be used after sterilization in patient in future.

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