Comparative evaluation of the antibacterial activity of Dill (Anethum graveolen L.) and garden Thyme (Thymus vulgaris) against of Salmonella Typhimurium (ATCC= 14028)

Subject Areas : Journal of Comparative Pathobiology

نازیلا Eslami, ¹ , , Y , Anzabi, ² , M.A Nour Azar, ³

1 - Graduate of Master in Microbiology, Faculty of Basic Sciences, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
2 - Assistant Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran
3 - Assistant Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran

Received: 2022-10-18 Accepted : 2023-01-19 Published : 2023-01-19

Keywords: Salmonella typhimurium, Dill, Garden Thyme, Antibacterial, Antimicrobial,

Abstract :

Salmonellosis is one of the most important common diseases of livestock and humans worldwide. With the prevalence of antibiotic resistance, the tendency to use medicinal plants to control microorganisms has increased. The aim of this research was to investigate the antibacterial effects of the essential oils of Thymus vulgaris and salmonella typhimurium (ATCC=14028). For this purpose, first, the essential oils of the mentioned plants were extracted by steam distillation using a Clovenger machine. Then, using the microdilution broth method, the Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) of essential oils alone and in combination, as well as drug sensitivity or resistance testing based on the well diffusion method in agar against the standard strain of Salmonella‌ typhimurium bacteria, were determined in comparison with three common antibiotics in veterinary medicine. The MIC was calculated for thyme (0.25), mixed essential oil (0.50), doxycycline (0.0078) and oxytetracycline (<0.00195) μg/ml. The MBC for them was 0.25, 0.50, 0.0156 and >0.00195 respectively. Dill essential oil and erythromycin antibiotic had no antimicrobial effect against Salmonella typhimurium. The highest effect was related to oxytetracycline (with a growth inhibition diameter of 27 mm) and the lowest effect was related to thyme essential oil (with a growth inhibition diameter of 10 mm). Unlike dill essential oil, garden thyme essential oil has a significant antibacterial effect.

References:

1. Humphrey T. Salmonella, stress responses and food safety. Nature Reviews Microbiology. 2004;2(6):504-9.


2. Griesche C, Baeumner AJ. Biosensors to support sustainable agriculture and food safety. TrAC Trends in Analytical Chemistry. 2020;128:115906.


3. Awang MS, Bustami Y, Hamzah HH, Zambry NS, Najib MA, Khalid MF, et al. Advancement in Salmonella Detection Methods: From Conventional to Electrochemical-Based Sensing Detection. Biosensors. 2021;11(9).


4. Rakitin AL, Yushina YK, Zaiko EV, Bataeva DS, Kuznetsova OA, Semenova AA, et al. Evaluation of Antibiotic Resistance of Salmonella Serotypes and Whole-Genome Sequencing of Multiresistant Strains Isolated from Food Products in Russia. Antibiotics. 2021;11(1):1.


5. Bajpai VK, Baek K-H, Kang SC. Control of Salmonella in foods by using essential oils: A review. Food Research International. 2012;45(2):722-34.


6. Eftekhar F, Zamani S, Yousefzadi M, Hadian J, Nezhadebrahimi S. Antibacterial activity of Zataria multiflora Boiss essential oil against extended spectrum β lactamase produced by urinary isolates of Klebsiella pneumonia. 2011.


7. Tiwari BK, Valdramidis VP, O’Donnell CP, Muthukumarappan K, Bourke P, Cullen P. Application of natural antimicrobials forfood preservation. Journal of agricultural and food chemistry. 2009;57(14):5987-6000.

8. Smith-Palmer A, Stewart J, Fyfe L. The potential application of plant essential oils as natural food preservatives in soft cheese. Food microbiology. 2001;18(4):463-70.


9. Ishikawa T, Kudo M, Kitajima J. Water-soluble constituents of dill. Chemical and pharmaceutical bulletin. 2002;50(4):501-7.


10. Junaidh K, Kumar M. A review on Anethum graveolens: Its role in human life. 2022.


11. Khan AA, Amjad MS. GC-MS analysis and biological activities of Thymus vulgaris and Mentha arvensis essential oil. Turkish Journal of Biochemistry. 2019;44(3):388-96.


12. Manandhar S, Luitel S, Dahal RK. In vitro antimicrobial activity of some medicinal plants against human pathogenic bacteria. Journal of tropical medicine. 2019;2019.


13. Baron S. Medical microbiology. 1996.


14. Ganfon H, Houvohessou J-P, Assanhou AG, Bankole HS, Gbenou J. Activité antibactérienne de l’extrait éthanolique et des fractions de Anogeissus leiocarpa (DC) Guill. Et Perr.(Combretaceae). International Journal of Biological and Chemical Sciences. 2019;13(2):643-51.


15. Ruangamnart A, Buranaphalin S, Temsiririrkkul R, Chuakul W, Pratuangdejkul J. Chemical compositions and antibacterial activity of essential oil from dill fruits (Anethum graveolens L.) cultivated in Thailand. Mahidol Univ J Pharm Sci. 2015;42(3):135-43.


16. Mohamed SH, Zaky WM, Kassem JM, Abbas HM, Salem M, Said-Al Ahl H. Impact of antimicrobial properties of some essential oils on cheese yoghurt quality. World Applied Sciences Journal. 2013;27(4):497-507.


17. Borumand A, Hamedi M, Emam Jomea Z, Razavi H, Gholmakani M. Investigation on the antimicrobial effects of essential oils from dill and coriander seeds on Staphylococcus aureus, Escherichia coli O 157: H7 an Salmonella typhimurium. J Iran Food Sci Technol Res J. 2008;4(1):1-10.


18. Ranjbarian P, Sadeghian S, Shirazi MH, Sarafnezhad A, Fazeli M, Amin G, et al. Survey of anti-bacterial effect of plant extracts (fennel-dill-caraway-cinnamon) by flow cytometry and disk diffusion. 2004.


19. Guillín Y, Cáceres M, Torres R, Stashenko E, Ortiz C. Effect of Essential Oils on the Inhibition of Biofilm and Quorum Sensing in Salmonella enteritidis 13076 and Salmonella typhimurium 14028. Antibiotics. 2021;10(10):1191.


20. Saffari Samani E, Jooyandeh H, Alizadeh Behbahani B. Evaluation of reciprocal pharmaceutical effect and antimicrobial activity of Shirazi thyme essential oil against some Gram-positive and Gram-negative bacteria. Food Science and Technology. 2020;17(104):1-11.


21. Mostafa AA, Al-Askar AA, Almaary KS, Dawoud TM, Sholkamy EN, Bakri MM. Antimicrobial activity of some plant extracts against bacterial strains causing food poisoning diseases. Saudi journal of biological sciences. 2018;25(2):361-6.


22. Sharafati Chaleshtori R, Mazroii Arani N, Taghizadeh M, Sharafati Chaleshtori F. Antibiotic resistance pattern of salmonella isolated from hamburgers and detection of their sensitivity to some essential oils. Journal of Mazandaran University of Medical Sciences. 2017;27(148):136-42.


23. Shahnazi S, Khalighi-Sigaroodi F, Ajani Y, Yazdani D, Ahvazi M, Taghizad-Farid R. Study on Chemical Composition and Antimicrobial Activity of the Essential Oil of Thymus trautvetteri Klokov & Desj.–Shost. Journal of Medicinal Plants. 2007;6(23):80-8.


24. Gebeyehu DT. The foodborne diseases antimicrobial resistance development in food animals: A case of Salmonella isolates from diarrheic sheep in and around Gondar city, Ethiopia: A cross-sectional study. 2020.


25. Lenchenko E, Blumenkrants D, Vatnikov Y, Kulikov E, Khai V, Sachivkina N, et al. Poultry Salmonella sensitivity to antibiotics. Systematic Reviews in Pharmacy. 2020;11(2):170-5.


26. Habibi H, Ghahtan N, Morammazi S. The effects of some herbal essential oils against Salmonella and Escherichia coli isolated from infected broiler flocks. J World Poult Res. 2018;8(3):74-80.


27. Chand B. Antibacterial effect of garlic (allium sativum) and ginger (zingiber officinale) against staphylococcus aureus, salmonella typhi, escherichia coli and bacillus cereus. Journal of microbiology, biotechnology and food sciences. 2013;2(4):2481-91.


28. DiMarzio M, Shariat N, Kariyawasam S, Barrangou R, Dudley EG. Antibiotic resistance in Salmonella enterica serovar Typhimurium associates with CRISPR sequence type. Antimicrobial agents and chemotherapy. 2013;57(9):4282-9