The role of MC3/MC4 melanocortin receptors on food intake induced by nesfatin-1 in broilers

Subject Areas : Journal of Comparative Pathobiology

A. Heidarzadeh, ¹ , مرتضی Zendehdel, ² , حسن Gilanpour ³ , وهاب Babapour, ⁴

1 - Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2 - Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
3 - Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.
4 - Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

Received: 2022-09-27 Accepted : 2022-12-03 Published : 2022-12-03

Keywords: Food Intake, Nesfatin-1, Melanocortin system, chicken,

Abstract :

Food intake is a set of physiological mechanisms affecting different central nervous system areas. The melanocortin system plays an important role in the central control of food intake in birds. Nesfatin-1 reduces food intake in birds. The present study was conducted to investigate the effect of the melanocortin system on food intake caused by nesfatin-1 in broiler chickens. 36 broilers were randomly divided into three experimental groups. Each experiment included a control group and 3 treatment groups (12 chickens in each group). In all experiments, after 3 hours of food deprivation (FD3), chicks received either control diluent or drug solution by intracerebroventricular (I.C.V.) injection. Then the birds had ad libitum access to the ‎food and fresh water, and then cumulative food intake (gr) was measured based on the ‎percentage of the body (%BW). In the first experiment, chickens in the first group were given the control solution, the second group nesfatin-1 (40 ng), the third group SHU9119 (0.5 nmol, antagonist of MC3/MC4 receptors), and the fourth group nesfatin-1 + SHU9119 intracerebroventricularly was injected. In the second experiment, chickens in the first group were injected with the control solution, the second group with nesfatin-1 (40 ng), the third group with MCL0020 (0.5 nmol, MC4 receptor antagonist), and the fourth group with nesfatin-1+ MCL0020. In the third experiment, chickens in the first group were injected with the control solution, the second group with nesfatin-1 (10 ng), the third group with MTII (2.45 pmol, an agonist of MC3/MC4 receptors), and the fourth group with nesfatin-1+ MTII. The results showed that the injection of nesfatin-1 with a dose of 40 ng decreased food intake (p<0.05). Co-injection of nesfatin-1 and SHU9119 and co-injection of nesfatin-1 and MCL0020 inhibited the hypophagia caused by nesfatin-1 (p < 0.05). Injection of Nesfatin-1 with a dose of 10 ng and MTII with a dose of 2.45 pmol did not affect food intake (p > 0.05). While the co-injection of nesfatin-1 with a dose of 10 ng and MTII with a dose of 2.45 pmol reduced food intake (p < 0.05). According to the results, food intake caused by nesfatin-1 in broilers is probably mediated by the melanocortin system.

References:

1. Denbow D. Food intake control in birds. Neuroscience & Biobehavioral Reviews. 1985;9(2):223-32.


2. Carew L, Evarts K, Alster F. Growth, feed intake, and plasma thyroid hormone levels in chicks fed dietary excesses of essential amino acids. Poultry Science. 1998;77(2):295-8.


3. Guo Ff, Xu L, Gao Sl, Sun Xr, Li Zl, Gong Yl. The effects of nesfatin‐1 in the paraventricular nucleus on gastric motility and its potential regulation by the lateral hypothalamic area in rats. Journal of neurochemistry. 2015;132(3):266-75.


4. Kohno D, Nakata M, Maejima Y, Shimizu H, Sedbazar U, Yoshida N, et al. Nesfatin-1 neurons in paraventricular and supraoptic nuclei of the rat hypothalamus coexpress oxytocin and vasopressin and are activated by refeeding. Endocrinology. 2008;149(3):1295-301.


5. Zendehdel M, Hamidi F, Babapour V, Mokhtarpouriani K, Fard RMN. The effect of melanocortin (Mc3 and Mc4) antagonists on serotonin-induced food and water intake of broiler cockerels. Journal of veterinary science. 2012;13(3):229-34.

6. Ahmadi F, Zendehdel M, Babapour V, Panahi N. CRF 1/CRF 2 and MC 3/MC 4 Receptors Affect Glutamate-Induced Food Intake in Neonatal Meat-Type Chicken. Brazilian Journal of Poultry Science. 2019;21.


7. Qi W, Ding D, Salvi RJ. Cytotoxic effects of dimethyl sulphoxide (DMSO) on cochlear organotypic cultures. Hearing research. 2008;236(1-2):52-60.


8. Ahmadi F, Zendehdel M, Babapour V, Panahi N, Hassanpour S, Khodadadi M. Modulatory function of NMDA glutamate receptor on MC3/MC4 receptors agonist-induced hypophagia in neonatal meat-type chicken. Veterinary research communications. 2017;41(4):241-8.


9. Heidarzadeh H, Zendehdel M, Babapour V, Gilanpour H. The effect of Nesfatin-1 on food intake in neonatal chicks: role of CRF1/CRF2 and H1/H3 receptors. Veterinary research communications. 2018;42(1):39-47.


10. Ghashghayi E, Zendehdel M, Khodadadi M, Rahmani B. Central dopaminergic, serotoninergic, as well as GABAergic systems mediate NMU-induced hypophagia in newborn chicken. International Journal of Neuroscience. 2022:1-11.


11. van Tienhoven At, Juhasz L. The chicken telencephalon, diencephalon and mesencephalon in stereotaxic coordinates. Journal of Comparative Neurology. 1962;118(2):185-97.


12. Saito E-S, Kaiya H, Tachibana T, Tomonaga S, Denbow DM, Kangawa K, et al. Inhibitory effect of ghrelin on food intake is mediated by the corticotropin-releasing factor system in neonatal chicks. Regulatory peptides. 2005;125(1-3):201-8.


13. Heidarzadeh H, Zendehdel M, Babapour V, Gilanpour H. The effect of Nesfatin-1 on food intake in neonatal chicks: role of CRF 1/CRF2 and H1/H3 receptors. Veterinary research communications. 2018;42(1):39-47.


14. van Koppen CJ, Kaiser B. Regulation of muscarinic acetylcholine receptor signaling. Pharmacology & therapeutics. 2003;98(2):197-220.


15. Finelli C, Martelli G, Rossano R, Padula MC, La Sala N, Sommella L, et al. Nesfatin-1: role as possible new anti-obesity treatment. EXCLI journal. 2014;13:586.


16. Abbasnejad M, Jonaidi H, Denbow D, Rahimi AP. Feeding and locomotion responses to centrally injected nociceptin/orphanin FQ in chicks. Physiology & behavior. 2005;85(4):383-6.


17. Xu L, Wang Q, Guo F, Pang M, Sun X, Gao S, et al. Nesfatin-1 signaling in the basom edial amygdala modulates the gastric distension-sensitive neurons discharge and decreases gastric motility via melanocortin 3/4 receptors and modified by the arcuate nucleus. European journal of pharmacology. 2015;764:164-72.


18. Stengel A, Taché Y. Role of brain NUCB2/nesfatin-1 in the regulation of food intake. Current pharmaceutical design. 2013;19(39):6955-9.