The effect of folic acid on sperm parameters on testicular ischemia-reperfusion injury in rat
Subject Areas : Journal of Comparative Pathobiology
Fakouri, A., Asghari, A., Akbari, Gh.
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Keywords: Testicle, Ischemia/ reperfusion, Folic Acid, Rat,
Abstract :
Ischemia reperfusion definition is the return of blood to ischemia tissue. In this study, the effects of folic acid as a drug, on testicular function in adult rats were studied after induction ischemia/reperfusion.In this study, 36 male Wistar rats, the weight range between 250-300gr, were selected and divided into 6 equal groups randomly.The Control group: The Group has not received any medication and every day the same volume of distilled water were administered like other groups andafter a week the abdominal cavity was opened and the left testicle was removed.The Sham group: The group has not received any medication andevery day the same volume of distilled water were administered like other groups and after a week the abdominal cavity was openedand after removal of testicle from the scrotum and replacing, the abdominal cavity reclosed .I/Rgroup:The group has not received any medication until indication of ischemia/reperfusion and every day the same volume of distilled water were administered like other groups and after 1 week the abdominal cavity was opened, and left testicular vein and artery was constricted for 1 hour thus the experimental ischemia reperfusion was induced and after 24 hour, the abdomen was opened and the left testis along with the left epididymis was extracted. 4th,5th,6th group: these groups were administered orally with (2, 5 & 10 mg/kg) of folic acid for 1 week, the abdominal cavity was opened, and left testicular vein and artery was constricted for 1 hour thus the experimental ischemia reperfusion was induced and after 24 hours the abdomen was opened and the left testis along with the left epididymis was extracted. After the rats were euthanized the epididymis were removed and then by scissors were cut into small pieces to allow sperm comfortable out there and immediately placed in 5 ml HBSS environment and for 20 minutes on a hot plate was incubated at 37°C and then evaluate sperm parameters (sperm count and sperm vitality and motility) were performed.The data were statistically analyzed and statistics on the percentage of progressive motility, sperm viability and sperm concentration per unit volume of folic acid showed significant difference (p<0.05) compared to I/R group.Based on the results obtained, it seemsFolic acid can behave appropriate and acceptable effects to Prevention of Ischemia-reperfusion injury in rat testis.