Antispasmodic effect of Ephedra major extract on adrenergic and calcium channels receptors in the rat jejunum
Subject Areas : Journal of Comparative Pathobiology
F. Mehrzad-Gilmalec,
1
(
دانشجوی دامپزشکی، دانشکده دامپزشکی، واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران
)
N. Panahi,
2
(
گروه فاماکولوژی، دانشکده دامپزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
)
G. Sadeghi- Hashjin,
3
(
گروه فارماکولوژی، دانشکده دامپزشکی، دانشگاه تهران، ایران
)
Keywords: Ephedra major Herb, Jejunum, Organ bath, Rat,
Abstract :
Tendency to utilize Ephedra containing products is increasing due to their favorable propertiessuch as weight loos, antibacterial, antisensetive etc. Due to the presence of gastro-intestinalsystem agonism α and β adrenergic compound and lack of systematic research on this issue, weconducted to determine effects of the hydroethanolic extract of Ephedra major (E. Major) hoston jejunum. Isolated jejunum from male Wistar rat were tested in the organ bath. Rats wereanesthetized and the jejunum was isolated and placed in Krebs solution. Then, it was cut intosegment of 1.5-2 cm and mounted in 25 ml chambers of organ bath. E. Major extract had arelaxation effect on rat jejunum. According to the results, the cumulative concentration of E.Major (0.00625 - 3 mg/ml) relaxed the KCl (80 Mm) - and acetylcholine (0.1 Mm) - inducedcontraction dose-dependently (P≤0.05). The inhibitory effect of extract on contraction inducedby KCl was unaffected by phenoxybenzamine (0.001 Mm). But verapamil (0.001 Mm) andpropranolol (0.001 Mm) inhibited the relaxant effect (P≤0.05). The inhibitory effect of extracton contraction induced by acetylcholine had synergism effect by verapamil, phenoxybenzamineand propranolol (P≤0.05). These findings revealed that β-adrenergic receptors and presumablyother channels such as Ca2+ channels involves in inhibitory effect of E. Major extract. Theresults support the possible beneficiary effects of this plant in traditional medicine.