The effect of in ovo injection of Gherlin on Sucrase enzyme activity of small intestine mucosa in broiler chicken

Subject Areas : Journal of Comparative Pathobiology

J.  Ghiasi Ghaleh-kandi, ¹ (دانشکده دامپزشکی، دانشگاه آزاد اسلامی واحد شبستر، شبستر، ایران)

Received: 2014-12-02 Accepted : 2015-03-09 Published : 2015-07-23

Keywords: In ovo injection, Ghrelin, Sucrase enzyme, Small intestine, Broiler chicken,

Abstract :

The aim of this study was to investigate the effect of in ovo administration of ghrelin onsubsequent small intestine Sucrase activity in broilers. In this experiment 900 fertilized eggswere collected from commercial breeder flock. The eggs were divided into five experimentalgroups; control (without injection), group 2 (in ovo injected with basal solution), group 3 (in ovoinjected with 50 μg/egg ghrelin), group 4 (in ovo injected with 100 μg /egg ghrelin) and group 5(in ovo injected with 150 μg /egg ghrelin). In ovo injection was done at day 7 of incubation.Two broilers from each group have been choose and slaughtered on days 21 and 42 of rearingperiod and 1, 10, 30, 50, 70 and 90 percent of small intestine sampled for evaluate Sucraseenzyme activity. According to the results, in ovo injection of 100 μg /egg ghrelin increasedintestine Sucrase enzyme activity in different parts of small intestine on day 21 post hatch(P<0.05) but had no effect on day 42 (P>0.05). These results suggest that in ovo injection of 100μg ghrelin had beneficial effect.