Effect of hydro-alcoholic extract of peel of Punica granatum L. on growth of Candida albicans in alloxan-induced diabetic rats

Subject Areas : Journal of Comparative Pathobiology

M. Sadeghpour, ¹ , M. Eidi, ² , F. Noorbakhsh, ³

1 - کارشناس ارشد میکروبیولوژی، گروه میکروبیولوژی، دانشکده علوم زیستی، واحد ورامین پیشوا، دانشگاه آزاد اسلامی، ورامین پیشوا، ایران
2 - دانشیار فیزیولوژی جانوری، گروه زیست شناسی، دانشکده علوم زیستی، واحد ورامین پیشوا،دانشگاه آزاد * اسلامی، ورامین پیشوا، ایران
3 - استادیار میکروبیولوژی، گروه میکروبیولوژی، دانشکده علوم زیستی، واحد ورامین پیشوا، دانشگاه آزاد اسلامی، ورامین پیشوا، ایران

Received: 2014-09-11 Accepted : 2015-01-05 Published : 2015-07-23

Keywords: Pomegranate (Punica granatumL., Candida albicans, Diabetes, Rat, Alloxan,

Abstract :

Nowadays, especially opportunistic fungi such as Candida albicans are the most common riskfactors in patients who are immunocompromised. Fungal infections caused by Candida speciesand increasing strains resistant to azole drugs in Immunosuppression patients are important toenjoy. The toxicity of drugs used, the resistance of the fungus and problems from druginteractions, necessitates the use of more effective and less toxic drugs cause the screw.Pomegranate consumption except for nutrition is impressive for medicinal and therapeuticeffects. The aim of this study was to investigate the effect of pomegranate peel extract at dosesconcentrations of 25, 50, 100 and 200 mg/kg on growth of C. albicans (ATCC 10231) inalloxan-induced diabetic rat. The animals were made diabetic by intraperitoneal injection ofalloxan (150 mg/kg). After diabetes induction, they were infected by Candida albicans, orally.Then, treatments of extract and Itraconazole were done orally. Sampling of fungus from month,culturing and colony counting were done daily for evaluating effects of extract and Itraconazoleon fungal growth. The results showed that oral treatment of hydro-alcoholic peel extractimproved Candida albicans infections in diabetic rats. Its potency is similar to Itraconazole, assynthetic drug. On the second day of treatment, significant effect of extract on growth C.albicans was shown (P<0.001). Comparison between effects of pomegranate extract andItraconazole on C. albicans was significant. The results suggest antifungal effect extract ofpomegranate compared with the antifungal medication. As a result, consumption ofpomegranate extract inhibited the growth of Candida albicans in the diabetic rats.