Development of Nardostachys jatamansi and Withania somnifera Formulation for Treatment of Sleep Disorders

Subject Areas : Journal of Chemical Health Risks

Anjan Adhikari ¹ , Subhadas Chatterjee ^{2 *} , Moumita Ray ³ , Rania Indu ⁴ , Sangita Bhattacharya ⁵ , Sankhadip Bose ^{6 *}

1 - Medical College Kolkata, 88 College St, College Square, Kolkata-700073, West Bengal, India
2 - Sanaka Educational Trust’s Group of Institutions, Department of Pharmacy, Durgapur, West Bengal, India
3 - Department of Pharmaceutical Technology, JIS University, 81, Nilgunj Road, Agarpara, Kolkata-700109, West Bengal, India
4 - Department of Pharmaceutical Technology, JIS University, 81, Nilgunj Road, Agarpara, Kolkata-700109, West Bengal, India
5 - Department of Clinical research, Micro Labs Limited, 58/3, Kudlu, Bangalore-560068, India
6 - The Neotia University, School of Pharmacy, South 24 Parganas, West Bengal, India

Received: 2025-01-23 Accepted : 2025-11-22 Published : 2025-09-28

Keywords: Sleep disorders, Withaferin A, Anxiety, Insomnia, Depression, Catalepsy,

Abstract :

Sleep is a state of decreased awareness of environmental stimuli that is distinguished from states such as coma or hibernation by its relatively rapid reversibility. Insomnia and other sleeping disorders are quite common in worldwide population nowadays. Although treatment with sedatives and hypnotics are available, still the safety of those medicaments are a big question. In this present study, 2 different formulations developed by Nardostachys jatamansi and Withania somnifera at 2 different proportions are made and evaluated for treatment of several sleep disorders. Isolation of marker compounds, evaluation of Pharmacokinetic parameters and evaluation of the safety and efficacy of those 2 test formulations were performed. From the obtained results, it could be inferred that both the formulations have potential therapeutic properties for prophylaxis and treatment of sleep disorders, along with no signs of toxicity for oral administration and other adverse effects which are associated with the use of traditional sedatives-hypnotics.Sleep is a state of decreased awareness of environmental stimuli, distinguished from states such as coma or hibernation by its relatively rapid reversibility. Insomnia and other sleeping disorders are quite common in the worldwide population nowadays. Although treatment with sedatives and hypnotics is available, the safety of those medications is a big question. In this present study, 2 different formulations developed by Nardostachys jatamansi and Withania somnifera at 2 different proportions are made and evaluated for the treatment of several sleep disorders. Isolation of marker compounds, evaluation of Pharmacokinetic parameters, and evaluation of the safety and efficacy of those 2 test formulations were performed. The results inferred that both formulations have potential therapeutic properties for prophylaxis and treatment of sleep disorders, as both formulations produced more than 50% sleep. Anxiety and cortisone levels were inhibited for more than 30% along with no signs of toxicity for oral administration at a 2g kg^-1 dose, and other adverse effects associated with the use of traditional sedatives-hypnotics. Moreover, a reduction in total neurotransmitter levels in the brain, elevated due to sleep deprivation was also observed when maintaining levels below 50%. From these results, it was concluded that both formulations have a potential activity against sleep-related disorders without potential toxicological effects. 

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