Effect of Aerobic Exercise and Cinnamon Extract on Leptin Gene Expression in Liver and Fat Tissues of Male Rats Fed by High Fat Diet
Subject Areas : Journal of Chemical Health Risks
Maria Dehvari
1
,
AbdolAli BanaeiFar
2
*
,
Sajad Arshadi
3
,
Ardeshir Zafari
4
1 - Department of Exercise Physiology, Faculty of Physical Education and Sports Sciences, South Tehran Branch, Islamic Azad University, Tehran, Iran
2 - Department of Exercise Physiology, Faculty of Physical Education and Sports Sciences, South Tehran Branch, Islamic Azad University, Tehran, Iran
3 - Department of Exercise Physiology, Faculty of Physical Education and Sports Sciences, South Tehran Branch, Islamic Azad University, Tehran, Iran
4 - Department of Physical Education and Sports Sciences, Zanjan Branch, Islamic Azad University, Zanjan, Iran
Keywords: Exercise, Obesity, Leptin, liver, Cinnamon, high-fat food,
Abstract :
Considering the desired effect of aerobic exercise, the effect of high-fat diets on obesity, and the beneficial effects of cinnamon; the present research seeks to answer the question of whether the implementation of aerobic exercise and cinnamon extract have different effects on the relative expression of leptin gene in the liver and fat tissues and also the mass of liver and fat tissues of male rats fed with a high-fat diet. The present experimental research was conducted using an animal model in the form of multi-group research (five groups: 1. control, 2. high-fat diet, 3. cinnamon extract and high-fat diet, 4. aerobic exercise and high-fat diet, 5. aerobic exercise and cinnamon extract and high-fat diet) Independent variables included aerobic exercise and cinnamon extract gavage of high-fat diet is also considered as a background variable. And Dependent variables are the level of leptin gene expression in the tissue of fat and liver, as well as body weight and the weight of liver and fat tissues. All groups received high_fat food in addition to normal food. The Training group participated in an aerobic exercise program. Cinnamon extract was given to cinnamon groups. All samples were anesthetized, killed, and operateon. The results show the effect of a high-fat diet on unfavorable changes in leptin gene expression, body weight, and the weight of fat tissue and liver. Performing aerobic exercise alone, consuming cinnamon extract alone, and the interaction of aerobic exercise and cinnamon extract cause desired changes in leptin gene expression in liver and fat tissue and the weight of liver and fat tissue compared to the high-fat diet group. Therefore; the Recommendation to take cinnamon extract along with exercise can cause desired and useful effects.
1. Bouassida A., Zalleg D., Bouassida S., 2006. Leptin, Its Implication in Physical Exercise and Training. Jssm. 5, 172-181.
2. Dishman R.K., Washburn R.A., 2004. Physical Activity Epidemiology. Champaign: Human Kinetics.
3. Le Mura L.M., Van Duvillard P., 2004. Clinical Exercise physiology. Philadelphia, LWW.
4. Patterson C.M., Dunn-Meynell A.A., Levin B.E., 2008. Three weeks of early-onset exercise prolongs obesity resistance in DIO rats after exercise cessation. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 294(2), 290-301.
5. Kang S., Woo J.H., Shin K.O., Kim D., Lee H.J., 2009. Circuit resistance exercise improves glycemic control and Adipokines in females with type II diabetes mellitus. Journal of Sports Sciences and Medicine. 8, 682-688.
6. Bergouignan A., Momken I., Schoeller D.A., Normand S., Zahariev A., Lescure B., Simon C., Blanc S., 2010. Regulation of energy balance during long-term physical inactivity induced by bed rest with and without exercise training. J Clin Endocrinol Metab. 95, 1045-1053.
7. Ossanloo P., Najar L., Zafari A., 2012. The Effects of Combined Training (Aerobic Dance, Step Exercise and Resistance Training) on Body Fat Percent and Lipid Profiles in Sedentary Females of AL_ZAHRA University. European Journal of Experimental Biology. 2(5), 1598-1602.
8. Eftekhari E., Zafari A., Gholami M., 2016. Physical activity, lipid profiles and leptin. The Journal of Sports Medicine and Physical Fitness. 56(4), 465-469.
9. Hagobian T.A., Sharoff C.G., Stephens B.R., Wade G.N., Silva J.E., Chipkin S.R., 2009. Effects of exercise on energy-regulating hormones and appetite in men and women. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 296(2), 233-42.
10. Messier C., Whately K., Liang J., Du L., Puissant D., 2007. The effects of a high-fat, high-fructose, and combination diet on learning, weight, and glucose regulation in C57BL/6 mice. Behavioral Brain Research. 178(1),139-145.
11. Halade G.V., Rahman M.M., 2010. High fat diet-induced animal model of age-association obesity and osteoporosis. The Journal of Nutritional Biochemistry. 21(12), 1162-1169.
12. Sampey B.P., Vanhoose A.M., Winfield H.M., Freeman A.J., Muehlbauer M.J., Fueger P.T., Newgard C.B., Makowski L., 2011. Cafeteria diet is a robust model of human metabolic syndrome with liver and adipose inflammation, Comparison to high fat diet. Obesity. 19(6), 1109-1117.
13. Bacci S., Menzaghi C., Ercolino T., Ma X., Rauseo A., Salvemini L., 2004. The +276 G/T single nucleotide polymorphism of the adiponectin gene is associated with coronary artery disease in type II diabetic patients. Diabetes Care. 27(8), 2015-2020.
14. Ouchi N., Parker J.L., Lugus J.J., Walsh K., 2011. Adipokines in inflammation and metabolic disease. Nature Reviews Immunology. 11(2), 85-97.
15. Nigro E., Scudiero O., Ludovica Monaco M., Palmieri A., Mazzarella G., Costagliola C., Bianco A., Daniele A.,2014. New Insight into Adiponectin Role in Obesity and Obesity-Related Diseases. 10, 1155.
16. Kyriazis G.A., 2005. The Effects of a Single Exercise Bout on Plasma Leptin Concentration in Obese Males. A thesis proposal submitted for the degree of Master of Arts in the College of Education at the University of Central Florida, Orlando.
17. Fernandes M.S., Silva L.d., Kubrusly M.S., Lima T., Muller C.R., Amé rico A., Fernandes .M.P, Cogliati B., Stefano J.T., Lagranha C.J., Evangelista F.S., Oliveira C.P., 2020. Aerobic Exercise Training Exerts Beneficial Effects Upon Oxidative Metabolism and Non-Enzymatic Antioxidant Defense in the Liver of Leptin Deficiency Mice. Front Endocrinol. 11, 502-588.
18. Takahashi H., Kotani K., Tanaka K., Egucih Y., Anzai K., 2018. Therapeutic Approaches to Nonalcoholic Fatty Liver Disease: Exercise Intervention and Related Mechanisms. Front Endocrinol. 9, 588. doi: 10.3389/fendo.2018.00588.
19. Mollazadeh H., Hosseinzadeh H., 2016. Cinnamon effects on metabolic syndrome: a review based on its mechanisms. Iran J Basic Med Sci. 19,1258-1270. http://dx.doi.org/10.22038/ijbms.2016.7906.
20. Khan A., Safdar M., Ali Khan M.M., Khattak K.N., Anderson R.A., 2003. Cinnamon improves glucose and lipids of people with type II diabetes. Diabetes Care. 26(12), 3215-3218.
21. Hasani-Ranjbar S., Nayebi N., Larijani B., Abdollahi M., 2009. A systematic review of the efficacy and safety herbal medicine used in the treatment of obesity. World Journal of Gastroenterology. 15(25), 3073.
22. Roussel A.M., Hininger I., Benaraba R., Ziegenfuss T.N., Anderson R.A., 2009. Antioxidant effects of a cinnamon extract in people with impaired fasting glucose that are overweight or obese. Journal of the American College of Nutrition. 28(1), 16-21.
23. Dylan W.P., Roshni C.G., Francesca S., Nichole E.L., 2009. Cinnamon Extract Inhibits Tau Aggregation Associated with Alzheimer’s disease. Journal of Alzheimer’s disease. 17, 585-597.
24. Chua M.T., Tung Y.T., Chang S.T., 2009. Antioxidant activities of extracts from the twigs Cinnamomum osmophloeum. Bioresource Technology. 99, 1918-1925.
25. Moslehy S.S., Husein K., 2009. Hepatoprotective effect of cinnamon extracts against carbon tetrachloride induced oxidative stress and liver injury in rats. Biol Res. 42, 93-98.
26. Badalzadeh R., Shaghaghi M., Mohammadi M., Dehghan G., Mohammadi Z., 2014. The effect of cinnamon extract and long-term aerobic training on heart function, biochemical alterations and lipid profile following exhaustive exercise in male rats. Coronary Artery Disease. 2, 8.
27. Shalaby M.A., Saifan H.A., 2014. some pharmacological effects of cinnamon and ginger herbs in obese diabetic rate. Journal of Intercultural Ethno pharmacology. 3(4), 144.
28. Sartorius T., Peter A., Schulz N., Drescher A., Bergheim I., Machann J., 2014. Cinnamon extract improves insulin sensitivity in the brain and lowers liver fat in mouse models of obesity. PloS One. 9(3),e92358.
29. Hamidpour R., Hamidpour M., Hamidpour S., Shahlari M., 2015. Cinnamon from the selection of traditional applications to its novel effects on the inhibition of angiogenesis in cancer cells and prevention of Alzheimer’s disease, and a series of function such as antioxidant, anti-cholesterol, antidiabetic, antibacterial, antifungal, nematicidal, acaracidal, and repellent activity. Journal of Traditional and Complementary Medicine. 5(2), 66-70.
30. Rashid Lamir A., Alizadeh A., Ebrahimi A.A., Dastani M., 2011. Effects of 4 Weeks of aerobic exercise with the consumption of cinnamon on lipoprotein parameters and blood glucose in type II diabetic women. Shahid Sadoughi University of Medical Sciences and Health Service. 20(5), 605-614.
31. Zou Y., Li J., Lu C., Wang J., Ge J., Huang Y., 2006 High-fat emulsion-induced rat model of nonalcoholic steatohepatitis. Life Sci. 79(11), 1100-7.
32. Machado M.V., Michelotti G.A., Xie G., Almeida P.T., Boursier J., Bohnic B., 2015. Mouse models of diet-induced nonalcoholic steatohepatitis reproduce the heterogeneity of the human disease. PloS One. 10(5), e0127991.
33. Ashrafy E., Hosseini S.E., 2018. Protective effects of Cinnamon hydro-alcoholic extract on liver lesions induced non-alcoholic fatty liver disease and sodium nitrite poisoning in adult male rats. J Shahrekord Univ Med Sci. 19(6), 13-23.
34. Sokhanvardastjerdi S., Banaeifar A., Arshadi S., Zafari A.,2020. The Effect of 12 Weeks Aerobic Training on PDX-1 and GLUT2 Gene Expression in the Pancreatic Tissue of Type 2 Diabetic Rats. Iranian Journal of Diabetes and Obesity. 12(2), 98-103.
35. Noura M., Arshadi S., Zafari A., Banaeifar A., 2020. The effect of running on positive and negative slopes on TNF-α and INF- γ gene expression in the muscle tissue of rats with Alzheimer’s disease. Journal of Basic Research in Medical Science. 7(1), 35-42.
36. Shang C., Lin H., Fang X., Wang Y., Jiang Z., Qu Y., Xiang M., Shen Z., Xin L., Lu Y., Gao J., Cui X., 2021. Beneficial effects of cinnamon and its extracts in the management of cardiovascular diseases and diabetes. Food Funct. 12, 12194–12220. DOI: 10.1039/d1fo01935j.
37. Rostami H., Tavakoli H.R., Yuzbashian E., Zarkesh M., Aghayan M., Hedayati M., Forouzanfar R., Mirmiran P., Khalaj A., 2018. The Association of Dietary Fat Sources with Leptin Gene Expression from Visceral and Subcutaneous Adipose Tissues among Tehranian Adults. Iranian Journal of Endocrinology and Metabolism. 20(4), 160-168.
38. Teng K.T., Nagapan G., Cheng H.M., Nesaretnam K., 2011. Palm olein and olive oil cause a higher increase in postprandial lipemia compared with lard but had no effect on plasma glucose, insulin and adipocytokines. Lipids. 46, 381-8.
39. Murakami K., Sasaki S., Takahashi Y., Uenishi K., Yamasaki M., Hayabuchi H., 2007. Nutrient and food intake in relation to serum leptin concentration among young Japanese women. Nutrition. 23, 461-468.
40. Yannakoulia M., Yiannakouris N., Blu-her S., Matalas A.L., Klimis Z.D., Mantzoros C.S., 2003. Body fat mass and macronutrient intake in relation to circulating soluble leptin receptor, free leptin index, adiponectin, and resistin concentrations in healthy humans. J Clin Endocrinol Metab. 88, 1730-1736.
41. Shen W., Wang C., Xia L., Fan C., Dong H., Deckelbaum R.J., 2014. Epigenetic modification of the leptin promoter in diet-induced obese mice and the effects of N-3 polyunsaturated fatty acids. Sci Rep. 4, 5282.
42. Romacho T., Glosse P., Richter I., Elsen M., Schoemaker M.H., Van T.E.A., 2015. Nutritional ingredients modulate adipokine secretion and inflammation in human primary adipocytes. Nutrients. 7, 865-886.
43. Miller G.D., Frost R., Olive J., 2001. Relation of plasma leptin concentrations to sex, body fat, dietary intake, and peak oxygen uptake in young adult women and men. Nutrition. 17, 105-11.
44. Kratz M., von Eckardstein A., Fobker M., Buyken A., Posny N., Schulte H., 2002. The impact of dietary fat composition on serum leptin concentrations in healthy nonobese men and women. J Clin Endocrinol Metab. 87, 5008-5014.
45. El Midaoui A., Haddad Y., Filali-Zegzouti Y., Couture R., 2017. Argan Oil as an Effective Nutri-Therapeutic Agent in Metabolic Syndrome:A Preclinical Study. Int J Mol Sci. 18, pii: E2492.
46. Jurado-Ruiz E., Varela L.M., Luque A., Berna G., Cahuana G., Martinez-Force E., 2017. An extra virgin olive oil rich diet intervention ameliorates the nonalcoholic steatohepatitis induced by a high-fat "Western-type" diet in mice. Mol Nutr Food Res. 61.
47. Chu N., Stampfer M., Spiegelman D., Rifai N., Hotamisligil G., Rimm E., 2001. Dietary and lifestyle factors in relation to plasma leptin concentrations among normal weight and overweight men. Int J Obes Relat Metab Disord. 25, 106-14.
48. Duque-Guimarães D.E., de Castro J., Martinez-Botas J., Sardinha F.L., Ramos M.P., Herrera E., 2009. Early and prolonged intake of partially hydrogenated fat alters the expression of genes in rat adipose tissue. Nutrition. 25, 782-789.
49. Pisani L.P., Oyama L.M., Bueno A.A., Biz C., Albuquerque K.T., Ribeiro E.B., 2008. Hydrogenated fat intake during pregnancy and lactation modifies serum lipid profile and adipokine mRNA in 21-day-old rats. Nutrition. 24, 255-261.
50. Anderson R.A., Qin B., Canini F., Poulet L., Rousse, A.M., 2013. Cinnamon Counteracts the Negative Effects of a High Fat/ High Fructose Diet on Behavior, Brain Insulin Signaling and Alzheimer-Associated Changes. PLoS One. 8(12), e 83243.
51. Kim S. M., Cho G.J., Yannakoulia M., Hwang T. G., Kim I. H., Park E.K., 2011. Lifestyle modification increases circulating adiponectin concentrations but does not change vaspin concentrations. Metabolism. 60(9). DOI: 10.1016/j.metabol.2011.01.013
52. Haidari F., Mohammadshahi M., Abiri B., Zarei M., Fathi M., 2020. Cinnamon extract supplementation improves inflammation and oxidative stress induced by acrylamide: An experimental animal study. Avicenna J Phytomed (AJP). 10(3), 243-252.
53. Ahmadi R., Omidali F., Pishghadam S., 2017. Investigating the effect of hydroalcoholic extract of cinnamon bark on blood leptin hormone levels in Wistar rats under air pollution. Animal Research Journal Iranian Biology Journal. 30(1), 13-21
54. Dyck D.J., 2005. Leptin Sensitivity in Skeletal muscle is modulated by Diet and Exercise. Exercise and Sport. DOI: 10.1097/00003677-200510000-00007.
55. Meier U., Gressner A.M., 2004. Endocrine Regulation of Energy Metabolism: Review of pathobiochemical and clinical chemical aspects of Leptin, Ghrelin, Adiponectin, and Resistin. Clinical Chemistry. 50, 1511-1525.
56. Alavizade N.S., Naghibzade A., Drodi S., Hoseni M., Sakhi A., Rashid Lamir A., 2018. Effect of Eight-week aerobic training combined with Green Coffee consumption on ABCG8 gene expression, serum Leptin and HDL levels of overweight women. J Neyshabur Univ Med Sci. 6(2), 72-81.
57. Rohner-Jeanrenaud F., 2002. Aspects of the Neuroendocrine Regulation of Body weight Homeostasis. American Endocrinology. 63, 125-128.
58. Gökbel H., Baltaci A.K., Üçok K., Okudan N., Moĝulkoç R., 2005. Changes in serum leptin levels in strenuous exercise and its relation to zinc deficiency in rats. Biological Trace Element Research. 106(3), 247-252.
59. Okazaki T., Himeno H., Nanri H., Ogata M., Ikeda M., 1999. Effects of mild aerobic exercise and a mild hypocaloric diet on plasma leptin in sedentary women. Clin Exp Pharmacol Physiol. 26(5-6), 415-20.