Identification of Bacterial Isolates of Pseudomonas fluorescens siderophore from Rhizosphere of Corn Fields and Evaluation of Iron Absorption by Corn

Subject Areas : Journal of Chemical Health Risks

Sayed Amin Fani Yazdi ¹ ( Department of Soil Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran )
Amir Fotovat ² ( Department of Soil Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran )
Amir Lakzian ³ ( Department of Soil Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran )
Ali Akbar Haddad Mashhadrizeh ⁴ ( Industrial biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran )

Received: 2018-09-16 Accepted : 2018-10-29 Published : 2018-10-29

Keywords: Single cross 704, Iron, Specific primer, PFG5 isolate,

Abstract :

Iron is one of the most important elements for plant growth and metabolism. Plant growth promoting bacteria such as Pseudomonas fluorescens isolates play an important role in the absorption of iron by plants under iron deficiency by production of microbial siderophore. In this study, 25 bacterial isolates were isolated from corn plants rhizosphere in Khorasan Razavi Province, Iran in 2017. Based on physiological-biochemical and molecular diagnostic methods, by using 16SPSEfluF and 16SPSER specific primers, bacterial isolates were identified as P. fluorescens. Among these isolates, the highest amount of siderophore production was related to the isolate PFD1 and the lowest to the isolate PFD3. Moreover, in the study of iron absorption by corn (Single Cress 704), the highest iron absorption was obtained from PFG5 treatment (283 ± 2/3 ppm) and the lowest from control treatment (7/1 ± 6/107 ppm). Isolate PFG5 showed the highest yield increase on fresh and dry weight of root and aerial parts among treatments. PFG5 isolates could be introduced as an effective isolate in iron deficiency conditions.

References:

1. Aguado-Santacruz G.A.A., Moreno-Gómez B.A., Jiménez-Francisco B.B., García-Moya E.B., and Preciado-Ortiz R.E., 2012. Impact of the microbial siderophores and phytosiderophores on the iron assimilation by plants: a synthesis. Journal of Microbiology. 35, 9ââ‚‌“21.


2. Ahmed E., Holmström S.J., 2014. Siderophores in environmental research: roles and applications. Microb Biotechnol. 7, 196-208.


3. Sah S., Singh N., Singh R., 2017. Iron acquisition in maize (Zea mays L.) using Pseudomonas siderophore. Journal Biotech. 7, 121-127.


4. Kalam S., Das S.N., Basu A., Podile A.R., 2017. Population densities of indigenous Acidobacteria change in the presence of plant growth promoting rhizobacteria (PGPR) in rhizosphere. Journal Basic Microbiol. 57, 376-385.


5. Becker J.O., Hedges R.W., Messens E., 2008. Inhibitory effect of pseudobactin on the uptake of Fe by higher plants. Canadian Journal of Microbiology. 49, 1090-1093.


6. Crowley D.E., Wang Y.C., Reid C.P.P., Szaniszlo P.J. 2009. Mechanism of iron acquisition from siderophores by microorganisms and plants. In Y Chen., Y Hadar., eds, Iron Nutrition artd Interaction in Plants. Kluwer Academic Publishers, Dordrecht, The Netherlands. pp. 213-232.


7. Jurkevitch E., Hadar Y., Chen Y., 2009. The remedy of limeinducedchlorosis in peanuts by Pseudomonas sp. siderophores . Journal of Plant Nutrition. 9, 535-545.


8. Maunsell B., Adams C., O'Gara F., 2006. Complex regulation of AprAmetalloprotease in Pseudomonas fluorescens M114: evidence for the involvement of iron, the ECF sigma factor, PbrA and pseudobactin M114 siderophore. Microbiology. 152, 29-42.


9. Bar-Ness E., Hadar Y., Chen Y., Shanzer A., Libman J., 2015. Iron Uptake by Plants from Microbial Siderophores. Plant Physiol. 99, 1329-1335.


10. Sharma A., Johri B., 2003. Growth promoting influence of siderophore-producing Pseudomonas strains GRP3A and PRS9 in maize (Zea mays L.) under iron limiting conditions. Microbiol Res. 158, 243-248.


11. Radzki W., Mañero F.G., Algar E., García J.L., García-Villaraco A., Solano B.R., 2013. Bacterial siderophores efficiently provide iron to iron-starved tomato plants in hydroponics culture. Cogent Food & Agriculture.104, 321-330.


12. Gomes L.H., Duarte K.M.R., Andrino F.G., Tavares F.C.A., 2014. A simple method for DNA isolation from Xanthomonas spp. African Journal of Microbiology Research. 57, 553-555.


13. Scarpellini M., Franzetti L., Galli A., 2004. Development of PCR assay to identify Pseudomonas fluorescens and its biotype. FEMS Microbiol Lett. 236, 257-260.


14. Silva-Stenico M.E., Pacheco F.T.H., Rodrigues J.L.M., Carrilho E., Tsai, S.M., 2005. Growth and siderophore production of Xylellafastidiosa under iron-limited conditions. Microbiology Research. 160, 429-436.


15. Carrillo-Castañeda G., Muñoz J.J., Peralta-Videa J. R., 2005. A spectrophotometric method to determine the siderophore production by strains of fluorescent Pseudomonas in the presence of copper and iron. Journal of Applied Microbiology. 81, 35-40.


16. Tahmasbi F., Lakzian A., Khavazi K., PakdinParizi A., 2014. Isolation, identification and evaluation of sidrophore production in Pseudomonas bacteria and its effect on hydroponically grown corn. Journal of Molecular and Cellular Research. 27(1), 78-87. (In Persian)


17. Crowley D.E., Reid C.P., Szaniszlo P.J., 2016. Utilization of microbial siderophores in iron acquisition by oat. Plant Physiology. 87, 680-685.


18. Mirmajlessi S.M., Destefanis M., Gottsberger R.A., Mänd M., Loit E., 2015. PCR-based specific techniques used for detecting the most important pathogens on strawberry: a systematic review. Syst Rev. doi: 10.1186/2046-4053-4-9.


19. Palleroni N.J., 2010. Present situation of the taxonomy of aerobic Pseudomonas. In: Pseudomonas Molecular Biology and Biotechnology (Galli, E., Silver, S. and Witholt, B., Eds.), American Society for Microbiology, Washington. Front Microbiol. 105ââ‚‌“115.


20. Mahmoud A., Abd-Alla M., 2001. Siderophore production by some microorganisms and their effect on Bradyrhizobium-Mung Bean symbiosis. International Journal of Agriculture and Biology. 3, 157-162.


21. Gull M., Hafeez F.Y., 2012. Characterization of siderophore producing bacterial strain Pseudomonas fluorescens Mst 8.2 as plant growth promoting and biocontrol agent in wheat. African Journal of Microbiology Research. 6, 6308-6318.


22. Leclère V., Beaufort S., Dessoy S., Dehottay P., Jacques P., 2009. Development of a biological test to evaluate the bioavailability of iron in culture media. Journal of Applied Microbiology. 107, 1598-1605.


23. Vansuyt G., Robin A., Briat J.F., Curie C., Lemanceau P., 2007. Iron acquisition from Fe-pyoverdine by Arabidopsis thaliana. Molecular Plant-Microbe Interactions. 20, 441-447.


24. Ahemad M., Kibret M. 2014. Mechanisms and applications of plant growth promoting rhizobacteria: current perspective. Journal of King Saud University - Science. 26, 1-20.


25. Radzki W., Mañero F.G., Algar E., García J.L., García-Villaraco A., Solano B.R., 2013. Bacterial siderophores efficiently provide iron to iron-starved tomato plants in hydroponics culture. International Journal of Agricultural Research. 104, 321-330.