Evaluation of Photosynthetic Activity, Carbohydrates, Proline and Essential Oils (Ocimum basilicum L.) under the Elicitor of β-Cyclodextrin Nanoparticles

Subject Areas : Journal of Crop Ecophysiology

Azadeh Loni ¹ , Sara Saadatmand ² , Hossien Lari Yazdi ³ , Alireza Iranbakhsh ⁴

1 - Department of Biology, Payame Noor University, Tehran, Iran
2 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - Department of Biology, Borujerd Branch, Islamic Azad University, Borujerd, Iran
4 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Received: 2020-12-12 Accepted : 2021-08-23 Published : 2023-01-21

Keywords: nanoparticles, Carbohydrate, Fluorescence, β-cyclodextrin, Photosynthetic efficiency,

Abstract :

Induction of engineered nanoparticles into medicinal plants causes resistance, enhances quantitative and qualitative performance and effective substances in them. In this study, β-cyclodextrin nanoparticles (β-CDNPS) were synthesized. The nanoparticle profile was determined by UV-VIS spectrum, transmission electron microscopy (TEM) and dynamic light scattering (DLS). Then different concentrations of β-CDNPs including 0, 10, 50, 100 mg / l were used for foliar spray of basil (Ocimum basilicumc.v. Keshkeni luvelou). The amount of chlorophyll pigments and basil essential oil was significantly different (P≤ 5%) compared to the control.The maximum amount of chlorophyll b was observed in 100ppm treatment. The results showed a significant reduction (P≤5%) of the minimum fluorescence value (Fo) in the treatment of 50 ppm compared to the control and two levels of 10 and 100 ppm of β-CDNPS. The level of 50 ppm significantly increased (P≤ 5%) the maximum fluorescence, variable fluorescence (Fv), photochemical efficiency of the photosystem compared to the control. Foliar spray of β-CDNPS caused changes in basil gas exchange. Soluble sugars and proline of basil leave and root in 50ppm treatment had a significant increase (P≤%5) compared to the control, so that insoluble sugars in leave and root showed the opposite trend. In the presence of β-CDNPS, phenylpropanoids and terpenoids concentration, in the basil showed a significant difference (P≤5%) compared to the control group. Induction of β-CDNPS nanoparticles in the biosynthesis pathway of secondary metabolites results in plant productivity Basil plant under the treatment of nano β-cyclodextrin, with concentration-dependent behavior increased yield per unit area. Therefore, foliar spray at the right concentration can act as an inducer while stimulating, preserving the active ingredients in basil and increase the content of essential oil for food and drug consumption.

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