Protein Profile in Wheat as Affected by Drought Stress and Nano-Chelate Potassium
Subject Areas :
Journal of Crop Ecophysiology
sodabeh jahanbakhsh
1
,
Nafiseh Asghari Asghari
2
,
Ali Ebadi
3
,
Nasibeh Tavakoli
4
1 - mohaghegh university
2 - M.Sc. Graduated of Agricultural Biotechnology. Faculty of Agriculture and Natural Resource, University of Mohaghegh Ardabili, Ardabil, Iran.
3 - Prof. Department of Agronomy and Plant Breading, Faculty of Agriculture and Natural Resource, University of Mohaghegh ardabili, Ardabil, Iran.
4 - Ph.D. Student of Crop Physiology. Department of Agronomy and Plant Breading, Faculty of Agriculture and Natural Rresource, University of Mohaghegh Ardabili, Ardabil, Iran.
Received: 2016-10-25
Accepted : 2017-01-25
Published : 2017-07-23
Keywords:
Drought stress,
Wheat,
amino acid,
Nano-chelated potassium,
Proteomics,
Abstract :
Tolerant systems in plants including wheat are affected by several chemical factors, like nano-chelate potassium. Use of nano-chelated potassium under drought stress reduces its negative effects and increase yield. To study protein profiles of wheat under drought stress, a factorial experiment based on completely randomized design with three replications was performed. The first factore was three levels of irrigation (85%, 60% and 35% field capacity), the second factor consisted of three wheat cultivars (Zagros, Chamran and Kuhdasht) and the third factor consisted of four nano-chelated potassium concentrations (zero, 25, 45، 65 ppm). The results showed that concentrations of proline and carbohydrates which play a major role in plant defense mechanisms due to nano-chelated potassium treatment were increased. Catalase and butpolyphenol oxidase activity decreased with increasing concentrations of nano-chelate potassium, while peroxidase activity increased. The results of two dimentional electrophoretic studies showed changes of protein expression, due to the effect of nano-chelated potassium as a direct contact with the defensive system against drought stresses, such as 11, 6, 5, 19, 14 bands. Nano-chelated potassium is also associated with proteins involved in the metabolism of carbohydrates and protein and final energy production.
References:
Agarwal, S., and V. Pandey. 2004. Antioxidant enzyme resposes to NaCl stress in Cassia angustifolia. Plant Biology. 48: 555-560.
Ahmadi, A., P. Ehsanzadeh, and F. Jabbari. 2009. Introduction of plant physiology. Vol 1. Translated of Hopkin-s. University of Tehran Press. 653P. (In Persian).
Anderson, L. 2005. Candidate-based proteomics in the search for biomarkers of cardiovascular disease. Journal of Physiology. 15: 563: 23-60.
Apel, K., and H. Heribert. 2004. Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annual Review of Plant Biology. 55: 373–399.
Arzani, A. 2008. Breeding of crop plants, fifth edition. Translate, Esfahan: Publication Center of Industrial Esfahan University. (In Persian).
Ashraf, M. 2009. Biotechnological approach of improving plant salt tolerance using antioxidants as markers. Biotechnology Advances. 27: 84-93.
Bates, L.S., R.P. Waldren, and I.D. Teare. 1973. Rapid determination of free prolin for water stress studies. Plant and Soil. 39: 205-208.
Beranova-Giorgianni, S. 2003. Proteome analysis by two-dimensional gel electroph and mass spectrometry: strength and limitation. Trends in Analytical Chemistry. 22: 273-81.
Bolen, D.W., and I.V. Baskakov. 2001. The osmophobic effect: Natural selection of a thermodynamic force in protein folding. Journal of Molecular Biology. 310(5): 955-963.
Bradford, M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Annual Biochenistery. 72: 248-254.
Chen, Q., L.M. Lauzon, A.E. DeRocher, and E. Vierling. 1993. Accumulation, stability, and localization of a major chloroplast heat-shock protein. Journal of Cell Biology. 110: 1873-1883.
Damerval, C., D.de. Vienne, M. Zivy, and H. Thiellement. 1986. Technical improvements in two-dimensional electrophoresis increase the level of genetic variation detected in wheat-seedling proteins. Electrophoresis. 7: 52-54.
Daneshian, J., Harvan Eslam, M. and Jonoubi, P. 2002. Study of quantitative and quality characteristics of soybean genotypes in deficit irrigation conditions. Iranian Journal of Crop Sciences. 11(3):393-409.
Edwards, R., D.P. Dixon, and V. Walbot. 2000. Plant glutathione S-transferases: Enzymes with multiple functions in sickness and in health. Trend Plant Science. 5: 193-198.
Gressel, J., and E. Galun. 1994. Genetic controls of photo- oxidant tolerance, pp. 237-274. In: Causes of photo-oxidative stress and amelioration of defense systems in plants. Foyer CH, Mullineaux PM, eds. CRC Press.
Hajduch, M., R. Rakwal, GK. Agrawal, and M. Yonekura Pretova. 2001. High-resolution twodimensional electrophoresis separation of proteins from metal-stressed rice (Oryza sativa L.) leaves: Drastic reductions/ fragmentation of ribulose-1,5-bisphosphate carboxylase/ oxygenase and induction of stress-related proteins. Electrophoresis. 22: 2824–2831
Hajiboland, R., and A. Joudmand. 2009. The K/Na replacement and function of antioxidant defense system. Acta Agriculturae Scandinavica Section B- Soil and Plant Science. 59(3): 246-259.
Hayat, S., and A. Ahmad. 2007. Salicylic acid: A plant hormone. Springer. 97-99.
Hirsch, J., K.C. Hansen, A.L. Burlingame, and M.A. Matthay. 2004. Proteomics: current techniques and potential applications to lung disease. American Journal of Physiology Lung Cell and Molecular Physiology. 287: 1-23.
Hong, Z., K. Lakkineni, Z. Zhang, D.P. Verma. 2000. Removal of feedback inhibition of delta (1)-pyrroline-5-carboxylate synthetase results in increased proline accumulation and protection of plants from osmotic stress. Plant Physiology. 122(4): 1129-1136.
Irigoyen, J.J., D.W. Emerich, and M. Sanchez-Diaz. 1992. Water stress inducedchanges in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa L.) plants. Plant Physiology. 84: 55-60.
Kar, M., and D. Mishra. 1976. Catalase, peroxidase, and poly phenol oxidase activities during rice leaf senescence. Plant Physiology. 57: 315-319.
KaviKishor, P.B., S. Sangam, R.N. Amrutha, P. Sri Laxmi, K.R. Naidu, and K. Rao. 2005. Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: Its implications in plant growth and abiotic stress tolerance. Current Science. 88(3): 424-438.
Kilili, K.G., N. Atanassova, A. Vardanyan, N. Clatot, K. Al-Sabarna, P.N. Kanellopoulos, A.M. Makris, and S.C. Kampranis. 2004. Differential roles of tau class glutathione S-transferases in oxidative stress. Journal of Biological Chemistery. 279: 24540–24551.
Kim, H., T.H. Lee, E.S. Park, J.M. Suh, S.J. Park, H.K. Chung, O.Y. Kwon, Y.K. Kim, H.K. Ro, and M. Shong. 2000. Role of peroxiredoxins in regulating intracellular hydrogen peroxide and hydrogen peroxide induced apoptosis in thyroid cells. Journal of Biological Chemistery. 275: 18266–18270.
Kim, S.Y., H.H. Jang, and J.R. Lee. 2009. Oligomerization and chaperone activity of a plant 2 Cys peroxiredoxin in response to oxidative stress. Plant Science. 177: 227–232.
Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227: 680-685.
Lovegrove, A., and R. Hooley. 2000. Gibberellin and abscisic acid signalling in aleurone. Science Ltd. 5: 1360-1385.
Magbanua, Z.V., C.M.D. Moraes, T.D. Brooks, W.P. Williams, and D.S. Luthe. 2007. Is catalase activity one of the factors associated with maize resistance to Aspergillus flavus? Molecular Plant Microbe Interact. 20(6): 697–706.
Manning, V.A., L.K. Hardison, and L. Ciuffetti. 2007. Ptr ToxA interacts with a chloroplast- localized protein. Molecular Plant Microbe Interactions. 20: 168- 177.
Mauch, F., B. Mauch-Mani, C. Gaille, B. Kull, D. Haas, and C. Reimmann. 2001. Manipulation of salicylate content in Arabidopsis thaliana by the expression of an engineered bacterial salicylate synthase. Plant Journal. 25: 67–77.
Mc Lean, M.D., D.P. Yevtushenko, A. Deschene, O.R.V. Cauwenberghe, A. Makhmoudova, J.W. Potter, A.W. Bown, and B.J. Shelp. 2003. Overexpression of glutamate decarboxylase in transgenic tobacco plants confers resistance to the northern root- knot nematode. Molecular Breeding. 11: 277-285.
Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science. 7: 405-410.
Mittler, R., S. Vanderauwera, M. Gollery, and F. Van Breusegem. 2004. Reactive oxygen gene network of plants. Trends Plant Science. 9(10): 490-498.
Moons, A. 2005. Regulatory and functional interactions of plant growth regulators and plant glutathione S-transferases (GSTs). Vitamins and Hormones Series. 72: 155–202.
Ohtsuka, K., D. Kawashima, Y. Gu, and K. Saito. 2005. Inducers and co-inducers of molecular chaperones. International Journal of Hyperthermia. 21(8): 703-11.
Shigeoka, S., T. Ishikawa, M. Tamoi, Y. Miyagawa, T. Takeda, Y. Yabuta, and K. Yoshimura. 2002. Regulation and function of ascorbate peroxidase isoenzymes. Journal of Experimental Botany. 53: 1305- 1319.
Singh Gill, S., and N. Tuteja. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants: A Review. Plant Physiology and Biochemistry. 48: 909-930.
Springer, N.M., C.A. Napoli, D.A. Selinger, R. Pandry, K.C. Cone, V.L. Chandler, and S. Kaeppler. 2003 Comparative analysis of SET domain proteins in maize and Arabidopsis reveals multiple duplications preceding the ivergence monocots and dicots. Plant Physiology. 132: 907-925.
Tasgın, E., O. Atici, and B. Nalbantoglu. 2003. Effects of salicylic acid and cold on freezing tolerance in winter wheat leaves. Plant Growth Regulation. 41: 231–236.
Tavakoli Hasanaklou1, H., A. Ebadi, and S. Jahanbakhsh. 2014. Study of some tolerance mechanisms to water dificit stress in bread wheat genotypes (Triticum aestivum L.). Cereal Research. 4(1): 13-25. (In Persian).
Thipyapong, P., J. Melkonian, D.W. Wolfe, and J.C. Steffens. 2004. Suppression of polyphenol oxidasesincreases stress tolerance in tomato. Plant Science. 167: 693–703.
Turkan, I., M. Bor, F. Ozdemir, and H. Koca. 2005. Differential responses of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolius Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Science. 168: 223-231.
Wang, C., Q.H. Ma, Z.B. Lin, P. He, and J.Y. Liu. 2008. Cloning and characterization of a cDNA encoding 14-3-3 protein with leaf and stem-specific expression from wheat DNA sequence. Plant Cell. 19: 130-136.
Wittmann-Liebold, B., H.R. Graack, and T. Pohl. 2006. Two-dimensional gel electrophoresis as tool for proteomics studies in combination with protein identification by mass spectrometry. Proteomics. 6: 4688-4803.
Zand, B., A. Soroushzadeh, F. Ghanati, and F. Moradi. 2009. Effect of Zn and IBA application on the some antioxodant enzymes activity in maize. Journal of Iranian Plant Biology. 2: 35-48.
Zhang, H., Sh. Zhang, Q. Meng, J. Zou, W. Jiang, and D. Liu. 2009. Effects of aluminum on nucleoli in root tip cells, root growth and the antioxidant defense systemin Vicia faba L. Acta Biologica Cracoviensia Series Botanica. 51: 99–106.
Zhou, Y., K.H. Kok, A.C. Chun, C.M. Wong, H. Wu, M.C. Lin, P.C. Fung, H. Kung, and D.Y. Jin. 2000. Mouse peroxiredoxin V is a thioredoxin peroxidase that inhibits p53-induced apoptosis. Biochemical and Biophysical Research Communications. 268: 921–927.
· Zhu, J.K. 2000. Genetic analysis of plant salt tolerance using Arabidopsis. Plant Physiology. 124: 941-948.
_||_