Vanadium cross-linked CMC-PEG-PLGA based on nanocomposite hydrogel with sodium alginate coating as a pH-sensitive insulin delivery system

Subject Areas :

Seyyed Nabiollah Hosseini ¹ , Asieh Abbassi Dolavi ² , Seyed Javad Zia-e-Haq ³ , Ayyub Saeedi ⁴

1 - Ph.D student in Department of Sport Physiology, Ayatollah Amoli Unit, Islamic Azad University, Amol, Iran
2 - Assistant Prof. of Sport Physiology, Department of Sport Physiology Unit Ayatollah Amoli, Islamic Azad University, Amol, Iran
3 - Assistant Prof. of Sport Physiology, Department of Sport Physiology, Shahroud Branch, Islamic Azad University, Shahrood, Iran.
4 - . Assistant Prof. of Life Sciences in Sport and Health, Department of Life Sciences in Sport and Health, Shahid Beheshti University, Tehran, Iran

Received: 2020-12-17 Accepted : 2020-12-17 Published : 2020-09-22

Keywords: CMC, Alginate, PEG, PLGA, Nanocomposite hydrogel, Vanadium nanoparticles, Insulin delivery,

Abstract :

: In recent years, significant advances have been made in the use of biomedical polymers as targeted carriers for the release of drugs, proteins, and growth factors. Vanadium (V) nanocomposite hydrogels were prepared in this study due to the formation of vanadium nanoparticles inside the swollen CMC hydrogels. The formation of vanadium (V) nanoparticles in hydrogels has been studied using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction patterns (XRD), scanning electron microscopy (SEM) experimental techniques. The XRD patterns analysis confirmed the formation of vanadium nanoparticles in a hydrogel matrix, and scanning electron microscopy micrographs also showed that the size of the nanoparticles was from 22 to 74 nm in the hydrogel matrix. The swelling behavior of nanocomposite hydrogels was studied at pH of 2.1 and 7.4. Hydrogels have shown better water absorption in less pH. Fourier transform infrared spectroscopy indicates the interaction between polysaccharides and the samples taken from the drug indicate that the drug-loaded peaks were loaded appropriately. With the release of drug from nanocomposite and pure hydrogel hydrogels, it was observed that its release rate in nanocomposite hydrogels was lower and the amount of its release decreased with increasing percentage of nanoparticles. In the cytotoxicity test after 24 h, the cell viability was in the range of 74.35% -96.05% (compared with the control sample, which was 100%). In the hydrogel nanocomposite sample containing insulin had the lowest cell viability of insulin 31.25 μg/ml after 24 h and it had the highest cell viability at 1000 (μg/ml) concentration with 25.7% cell cytotoxicity compared to the control group.

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