Investigating in vitro culture of Abu Jahl watermelon plant Citrullus colocynthis (L.) Schrad

Subject Areas : Genetic

سورمه Qara Matrosyan ¹ , مه‌لقا Ghorbanli ²

1 - Department of Biology, Islamic Azad University, Gorgan Branch
2 - Department of Biology, Islamic Azad University, Gorgan Branch

Received: 2006-07-14 Accepted : 2007-05-13 Published : 2007-05-22

Keywords: Naphthalene acetic acid, organogenesis, IAA, benzyl aminopurine, quintine, Abu Jahl watermelon,

Abstract :

In this research, the determination of the best cultivation environment for stem organogenesis and the selection of the most suitable cultivar from Abu Jahl watermelon seeds have been studied. In order to obtain sterile roasted seeds, the inhibition of the seed shell was removed in concentrated sulfuric acid for 20 minutes. After washing with sterile distilled water, the seeds were transferred to a sterile culture medium. 20 days later, small pieces (cotyledon, terminal bud, cotyledon axis and root) were prepared from the roasted seeds and transferred to MS medium with different hormone treatments. All isolated cultures produced callus, and only the terminal bud treated with IAA and quintine equal to 1 mg in relitertolide produced callus, then it was differentiated into bud and branch. To determine the most suitable cultivation environment; Two cultivation environments were considered. MS basic culture medium with IAA and quintine treatment equal to 1 mg and MS basic culture medium, vitamin 2 equal to MS basic medium and benzyl aminopurine treatment 2 mg/liter along with naphthalene acetic acid 0.1 mg/liter. The terminal bud was separated from the sterile culture medium and placed in these two culture mediums. The design was based on a completely random block. Data collection was done in four stages. The ability to produce callus in the culture medium treated with benzyl aminopurine along with naphthalene acetic acid was higher than in the culture medium of IAA and quintine. In the cultivation environment with IAA and quintine treatment with equal amounts, branching and increase in the length of the branches were significant. In the culture medium, treatment with benzyl aminopurine along with naphthalene acetic acid caused the differentiation of callus into branches, but branching and increasing the length of branches were not significant.

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