In vitro culture of carnation and acclimatization of the plantlet to ex vitro condition
Subject Areas : Geneticمه لقا ghorbanli 1 , ف nouri-cootanai 2 , ب Allahverdi Mamaghani 3
1 - Department of Biology, Islamic Azad University, North Tehran Branch, Iran
2 - Department of Biology, Islamic Azad University, North Tehran Branch, Iran
3 - Research Institute of Forest and Rangland, Tehran, Iran
Keywords: nutrient solution, Plant growth regulator, Dianthus caryophyllus var cerise royallette, Micropropoagation,
Abstract :
Dianthus caryophyllus is one of the most important species in Dianthus genus. In order to propagation virus-free plant material, in vitro culture of this species var cerise royallette were studied. Explants were cultured in Anderson medium containing 1 mgl-1 6-benzylaminopurine. 6-benzylaminopurine alone induced callus in nodes and internodes explants. To achieve organogenesis, calli subcultured onto Anderson medium with or without plant growth regulator. The highest shoot length was obtained in medium without any hormone. The most suitable adventitious shoot regeneration medium was Anderson medium supplement with 4 mgl-1 Kin and 0.1 mgl-1 NAA. Root regeneration was observ ed in medium with 0.5 and 1 mgl-1 naphtalenacetic acid. Rooted plantlet transferred into pot with perlite and irrigated by different nutrient solution. The effect of different nutrient solution (An, OM, MS, MC) on growth and development of plantlet were compared. The results showed Anderson medium was more effective than the others. Acclimatized plants were transferred to soil. All plants were survived in field condition and produced flower after 4 months.
Anderson W C, (1975) Tissue culture propagation of Rhododendrons in vitro. 14: 334 (abs).
Casanova E, valdes AE, Fernandez B, Moysset L, Trillas, M I (2004) Levels and immunolocalization of endogenous cytokinins in thidiazuron–induced shoot organogenesis in carnation, Journal of Plant Physiology, 161 (1): 95-104.
Genkov T, Tsoneva P, Ivanova I, (1997) Effect of cytokinins on photosynthetic pigments and chlorophyllase activity in in vitro cultures of auxillary buds of Dianthus caryophyllus L. Journal of Plant growth regulation, 16 (3): 169-172.
Hammatt N, Grant NJ, (1997) Micropropagation of mature british wild cherry, Plant cell, Tissue and Organ Culture, 47: 103-110.
Fal MA, Majada JP, Gonzalez A, Sanches Tames R, (1999). Difference between Dianthus caryophyllus L. cultivar in in vitro growth and morphogenesis are related to their ethylene production. Plant growth regulation, 27(2): 131-136.
Jethwani V, Kothari S L, (1996) phenylacetic acid induced organogenesis in cultured leaf segments of Dianthus chinensis. Plant Cell Report, 15 (11): 869-872.
Leshem B, (1986) Carnation Plantlet from vitrified plants as a source of somaclonal variation. Hort Science, 21: 320-321.
Nontaswatsri C, Fukai S, Touma T, Goi M, (2002). Compartion adventitious shoot formation from node and leaf explants of various carnation Dianthus caryophyllus L. cultivars, The journal of horticultural science and biotechnology. 77(5): 520-525.
Nugent, G., Wardley-Richardson, T. and Lu, C. (1991) Plant regeneration from stem and petal of carnation (Dianthus caryophyllus L.), Plant Cell Report, 10(9):477-480.
Majada JP, Tadeo F, Fal M.A, Sanchez-Tames R, (2000) Impact of culture vessel ventilation on the anatomy and morphology of micropropagated carnation. Plant cell, Tissue and Organ Culture, 63 (3), 207-214.
Miller RM, Kaul V, Hutchinson JF, Richards D, (1991a) Adventitious shoot regeneration in carnation (Dianthus caryophyllus L.) from auxillary bud explants. Annals of Botany, 67(1): 33-42.
Miller RM, Kaul V, Hutchinson JF, Mahesvaran G, Richards D, (1991b) Shoot regeneration from fragmented flower buds of carnation (Dianthus caryophyllus). Annals of Botany, 68(6): 563-568.
Misra P, and Chaturvedi H C, (1984) Micropropagation of Rosmarinus officinalis L. Plant cell, Tissue and Organ Culture, 3: 163-168
Murashige T, and Skoog F, (1962) Revised medium for rapid growth and bioassay with tobacco tissue culture, Physiologia Plantarum,15: 473-479
Podwyszynska M, (2003) Cell Tissue and Organ culture (Rooting of micropropagated shoot). In: Roberts AV, Debener T, and Gudin S, Encyclopedia of Rose science. Elsevier Press.
Pospislova J, Ticha I, Kadlecek P, Haisel D, (1999) Acclimatization of micropropagated plants to ex vitro condition. Biologia Plantarum, 42(4): 481-497.
Saher S, Piqeras A, Hellin E, Olmos E, (2004) Hyperhhydricity in micropropagated carnation shoots: the role of oxidative stress. Physiologia Plantarum, 120 (1); 152-161.
Saher S, Piqeras A, Hellin E, Olmos E, (2005) Prevention of hyperhydricity in micropropagated carnation shoots by bottom cooling : implications of oxidative stress, Plant cell, Tissue and Organ Culture, 81(2): 149-158.
Yadav MK, Gaur AK, Garg GK, (2003) Development of suitable protocol to overcome hyperhydricityin carnation during micropropagation, Plant cell, Tissue and Organ Culture, 72(2): 153-156.
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Anderson W C, (1975) Tissue culture propagation of Rhododendrons in vitro. 14: 334 (abs).
Casanova E, valdes AE, Fernandez B, Moysset L, Trillas, M I (2004) Levels and immunolocalization of endogenous cytokinins in thidiazuron–induced shoot organogenesis in carnation, Journal of Plant Physiology, 161 (1): 95-104.
Genkov T, Tsoneva P, Ivanova I, (1997) Effect of cytokinins on photosynthetic pigments and chlorophyllase activity in in vitro cultures of auxillary buds of Dianthus caryophyllus L. Journal of Plant growth regulation, 16 (3): 169-172.
Hammatt N, Grant NJ, (1997) Micropropagation of mature british wild cherry, Plant cell, Tissue and Organ Culture, 47: 103-110.
Fal MA, Majada JP, Gonzalez A, Sanches Tames R, (1999). Difference between Dianthus caryophyllus L. cultivar in in vitro growth and morphogenesis are related to their ethylene production. Plant growth regulation, 27(2): 131-136.
Jethwani V, Kothari S L, (1996) phenylacetic acid induced organogenesis in cultured leaf segments of Dianthus chinensis. Plant Cell Report, 15 (11): 869-872.
Leshem B, (1986) Carnation Plantlet from vitrified plants as a source of somaclonal variation. Hort Science, 21: 320-321.
Nontaswatsri C, Fukai S, Touma T, Goi M, (2002). Compartion adventitious shoot formation from node and leaf explants of various carnation Dianthus caryophyllus L. cultivars, The journal of horticultural science and biotechnology. 77(5): 520-525.
Nugent, G., Wardley-Richardson, T. and Lu, C. (1991) Plant regeneration from stem and petal of carnation (Dianthus caryophyllus L.), Plant Cell Report, 10(9):477-480.
Majada JP, Tadeo F, Fal M.A, Sanchez-Tames R, (2000) Impact of culture vessel ventilation on the anatomy and morphology of micropropagated carnation. Plant cell, Tissue and Organ Culture, 63 (3), 207-214.
Miller RM, Kaul V, Hutchinson JF, Richards D, (1991a) Adventitious shoot regeneration in carnation (Dianthus caryophyllus L.) from auxillary bud explants. Annals of Botany, 67(1): 33-42.
Miller RM, Kaul V, Hutchinson JF, Mahesvaran G, Richards D, (1991b) Shoot regeneration from fragmented flower buds of carnation (Dianthus caryophyllus). Annals of Botany, 68(6): 563-568.
Misra P, and Chaturvedi H C, (1984) Micropropagation of Rosmarinus officinalis L. Plant cell, Tissue and Organ Culture, 3: 163-168
Murashige T, and Skoog F, (1962) Revised medium for rapid growth and bioassay with tobacco tissue culture, Physiologia Plantarum,15: 473-479
Podwyszynska M, (2003) Cell Tissue and Organ culture (Rooting of micropropagated shoot). In: Roberts AV, Debener T, and Gudin S, Encyclopedia of Rose science. Elsevier Press.
Pospislova J, Ticha I, Kadlecek P, Haisel D, (1999) Acclimatization of micropropagated plants to ex vitro condition. Biologia Plantarum, 42(4): 481-497.
Saher S, Piqeras A, Hellin E, Olmos E, (2004) Hyperhhydricity in micropropagated carnation shoots: the role of oxidative stress. Physiologia Plantarum, 120 (1); 152-161.
Saher S, Piqeras A, Hellin E, Olmos E, (2005) Prevention of hyperhydricity in micropropagated carnation shoots by bottom cooling : implications of oxidative stress, Plant cell, Tissue and Organ Culture, 81(2): 149-158.
Yadav MK, Gaur AK, Garg GK, (2003) Development of suitable protocol to overcome hyperhydricityin carnation during micropropagation, Plant cell, Tissue and Organ Culture, 72(2): 153-156.