Enhancing bioavailability of lead for phytoremediation of contaminated soils

Subject Areas : Genetic

Zahra Arabi ¹ , مهدی Homaee ² , M.E Asadi ³

1 - Dep. Soil Science, Azad University, Science and Research Branch, Tehran, Iran.
2 - Dep. Soil Science, Tarbiat Modares University, Tehran, Iran.
3 - Soil and Water Research Scientist, Gorgan, Iran.

Received: 2008-11-22 Accepted : 2009-04-08 Published : 2009-09-16

Keywords: Soil Pollution, radish, Lead, phytoextraction, Synthetic chelators,

Abstract :

In this study the influence of enhancing synthetic chelate (HEDTA) were investigated on lead (Pb) solution in contaminated soils. The Pb phytoextraction capability of radish (Raphanus sativus L.) before and after chelating was also studied. The experiment was conducted in a randomized complete factorial design, with three replicated for each treatment. The Pb treatments (as PbCl2) were consisted of 0 (control), 50, 100, 600 and 1000 mg Pb kg-1 soil. When plants were fully grown, the chelating agent HEDTA was added to Pb treatments in concentration of 1.5, 5 and 6 mMkg-1 soil, respectively. The control treatments was received no chelate. Ten days later, some samples were taken from the plants and soils to measure lead concentrations after applying the chelate. The results indicated that in all treatments, the concentrations of soluble Pb in soil were more than the control treatment. In all treatments, Pb concentrations in plant shoots and roots were increased by increasing Pb concentrations in the soil solution. In current study, Pb concentration in radish shoots and roots, after enhancing HEDTA was larger than control. The collected data were also showed that Pb uptake by radish roots was larger than that of shoots.

References:

مؤسسه تحقیقات خاک و آب. (1375). روش‌های تجزیة گیاه، نشریه‌ی شماره 982

Alkorta, I., Herna´ndez-Allica, J., Becerril, J.M., Amezaga, I., Albizu, I.,Onaindia, M., Garbisu, C., (2004b). Chelate-enhanced phytoremediation of soils polluted with heavy metals. Rev. Environ. Sci. Biotechnol.3, 55–70.

Blaylock, M.J., Salt, D.E., Dushenkov, S., Zakharova, O., Gussman, C., Kapulnik, Y., Ensley, B.D., (1997). Enhanced accumulation of Pb in Indian mustard by soil-applied chelating agents. Environ. Sci. Technol. 31, 860–865.

Chaney, R.L., Brown, S.L., MLik, Y.M., Angle, J.S., Brewer, E.P., Baker, A.J. (1997). Phytoremediation of soil metals. Curr. Opin. Biotech. 8, 279-284.

Cunningham, S.D., Berti, W.R., Huang, J.W. (1995). Phytoremediation of contaminated soils. Trends Biotechnol. 13, 393–397.

Epelde, L., Hernandez, J., Becerril, J., Blanco, F., Garbisu, C. (2008). Effect of chelates on plants and EDDS for lead phytoextraction. J. Science of the total environment 401, 21-28.

Huang, J.W., Chen, J., Berti, W.B., Cunningham, S.D. (1997). Phytoremediation of lead-contaminated soils: role of synthetic chelates in lead phytoextraction. Environ. Sci. Technol. 31, 800–805.

Lasat, M.M. (2000). Phytoextraction of metals from contaminated soil: A review of plant/soil/metal interaction and assesment of pertient agronomic Issues. Journal of hazardous substance research, 2:5-21.

Neugschwandtner, R.W., Tlustoš, P., Komárek, M., Száková, J. (2008). Phytoextraction of Pb and Cd from a contaminated agricultural soil using different EDTA application regimes: Laboratory versus field scale measures of efficiency. J. Geoderma 144, 446–454.

Raskin, I., Smith, R.D., and Salt, D.E. (1997). Phytoremediation of metals: using plants to remove pollutants from the environment. Curr. Opin. Biotechnol. 8, 221–226.

 Santos, F.S., Javier, H.A.B., Nelson, A.S., Nelson, M., Carlos, G. (2006). Chelate-induced phytoextraction of metal polluted soils with Brachiaria decumbens. Chemosphere 65 43–50.

Van Engelen, D.L., Sharpe- Pelder, R.C., Moorhead, K.K. (2007). Effect of chelating agents and solubility of cadmium complexes on uptake from soil by Brassica juncea. Chemosphere 68, 401-408.