Interaction of Copper, Ascorbate and Gibberllin on germination, ion content, proline, soluble sugar and starch content two cultivars of rape seed (Brassica napus L.)
Subject Areas : Geneticحسین LariYazdi 1 , مه لقا Ghorbanli 2 , میترا Roshani 3
1 - Assinstant professor, department of biology, Isalmic Azad University, Boroujerd
2 - Assinstant professor, department of biology, Isalmic Azad University, Gorgan
3 - - Assinstant professor, department of biology, Isalmic Azad University, Boroujerd,
Keywords: Stress, Copper, Brassica napus L, Ascorbate, Gibberllin,
Abstract :
Copper is one of the heavy toxic metals that its toxicity is currently under investigation, in different plant types considering physiologic, biological, molecular, cellular and genetic aspects. In this research the copper toxicity effect on some of physiological activity of "Brassica napus L." Hylo 401 and RGS has been student. Seed of Brassica were cultured in hydroponic environmental. Roots and shoots used for evaluation of different treatments: Cu (5, 15, 25μm/l), Cu & AsA (0.5μm/l), Cu & GA3 (0.05μm/l) and Cu, AsA & Ga3 (as above), experiments were done in three replications in each treat group. In both Hyola 401 and RGS following factors were decreased significantly (p<0.05); germination percent, unsoluble sugar, K+ ions (in shoot and root). In both Hyola and RGS following factors were increased significantly (P<0.05) soluble sugar, proline. The finding of the study suggest that upon copper stress, in majority of studied parameters, Hyola 401 was more resistant than RGS. AsA treatment accompanying Cu, attenuated Cu effects on Brassica seedling, however GA3 exaggerated the Cu effects. Collected data were exracted and statistical analysis with SPSS statistical software 14th edition, performed. The results suggest that Cu induces inhibitory effects on growth and some of physiologic activities of plnant.
قربانلی، م. (1383) فیزیولوژی گیاهی3، انتشارات دانشگاه پیام نور
Brigezu, K., Lichtenberger, O., Leopol, I., Neumann, D., 1999, Heavy metal tolerance of Silene vulgaris, J plant physiol, 158:536-546.
Badr-Alaoui, Patricia Genet, Florence Vinit Dunand, Marie-Laure Toussaint, Daniel Epron and Pierre-Marie Badot, 2003, Effect of copper on growth in cucumber plants (Cucumis sativus) and its relationships with carbohydrate accumulation and change in ion contents, Plant Science Vol., 166:1213-1218
Barcelo, Je., Poschenrieder, C., 1990, Plant water relations as affected by heavy metals: a review. J plant Nutr., 1:1-37.
Bates, L. S., Waldren, R. P., Treare, I. D., 1973, Rapid determination of free proline for water-stress studies, plant soil., 39-205-207.
Bewley, J. D., Black, M., 1994, Seeds: physiology of development and germination. Second edition. Plenum press, New York.
Clarkson, D. T. and Luttge, U., 1989, Divalent Cations, Transport and compartmentation, Prog. Bot., 51:93-112.
Defilippis, L. F., 1979, The effect of heavy metal compounds on the permeability of chlorella cells. Z. Pflanzenphysiol, 92-39-49.
Delong, J. M., Steffen, K. L., 1997, Photosynthetic function, lipid peroxiation and alpha tocopherol content in spinach leaves during exposure to UVB radiation, Canadian journal of plant science., 77:453-459.
Greger, M., Bertell, G., 1992, Effects of Ni2+ and Cd2+ on the carbohydrate metabolism in sugar beet (Beta vulgaris)., J Exp Bot., 43:467-173.
Guo, Z., Tan, H., Zhu, Z., Lu, S., 2005 Effect of intermediates on ascorbic acid and oxalate biosynthesis of rice and in relation to its stress resistance, plant physiol & Bioch. 43: 955-962.
Harberd, N. P., Peng, J., 2002, The role of GA-mediated signaling in the control of germination. Science. 5:376-381.
Hendry GAF, Baker AJM, Ewart CF, 1992, Heavy metal tolerance and toxicity, oxygen radical processes and molecular damage in Cadmium tolerant clones of Holcus lanatus L. Acta Bot Neerl., 41:271-281.
Hippeli, S., Elstner, E. F., 1996, Mechanism of oxygen activation during plant stress: Bio Chemical effect of air pollutants. Journal of Plant physiology., 148:249-257.
Ibarra-Caballero J, Villanueva- Verdu Zco J, Molina-Galam J, J imerez ES-D, 1988, Proline accumulation as a symptom of drought stress in maize: a tissue differentiation requirement. Journal of Experimental Btany., 89:889-897.
Kochert, G., 1978, Carbohydrate determination by the phenol sulfuric acid method, In Helebust, J. A., Craig, J. S(ed): Hand book of phycological Methods., pp: 96-97 carbridge unir. Press, Cambridge.
Fanimokan, V. O., Adedeji, F. O., 1984, Copper deficiency and toxicity in two tropical leaf vegetables (Celosia argentea L. and Amaranthus dubius mart. ex thell), Envir & Exper. Botany., 24:105-110.
Mehta, Sk., Cour, J. P., 1999, Heavy metal-induced proline accumulation and its role in ameliorating metal toxicity in Chlorella vulgaris., New phytol., 143:253-259.
Mishra, A., Choudhuri, MA., 1997, Differential effects of pb2+ and Hg2+ on inhibition of germination of seed of two cultivars. Indian Journal of plant physiology 2(1)
Moya, J. L., Ros, R., Picazo, I., 1993, Influence of Cademium and Nickel on growth, net photosynthesis and carbohydrate distribution in rice plants, Photosynthesis research., 36:75-80.
Naidu, C. V., Rajendrudu, G., Swamy, P. M., 2000, Effect of plant growth regulators on seed germination of Sapindus trifoliatus. Seed Science and Technology., 28:249-252.
Palanca, J., et al, 2000, The control of As synthesis and turn over in pea seedling. J. Exp. Botany., 345-674.
Pandey, N., Sharma, C. P., 2002, Effect of heavy metal Co2+ Ni2+ and Cd2+ on growth and metabolism of cabbage, Plant Sci, 163: 753-758.
Pandolfini, T., Gabbrielli R, Commparini, C., 1992, Nickel toxicity and peroxidase activity In seedings of Triticum aestivum L., Plant Cell Environ., 15:719-725
Poschenrieder, C. B., Gunse, Barcelo. J., 1989, Influence of heavy metals on water relations, stomatal resistance, and abscisic asid content in expanding bean leaves, plant physiol., 90:1365-1371.
Stearns, J. C., Shah, S., Greenberg, B. M., Oixon, D. G., Glick, B. R., 2005, Tolerance of transgenic canola expressing 1- amino cyclopropane- 1- Carboxylic acid deaminase to growth inhibition by nickel, Plant Physiology, Bichem, 41: 701-708.
Vincent, W. F., Roy, S., 1993, Solar ultraviolet-B radioation and aquatic primary production: damage, protection and recovery Environmental reviews 1:1-12.
|
Wain wright, S. J., Woolhouse, H. W., 1977, Some physiological Aspects of Nickel and Zinc tolerance in Agrostis tenuis: Cell Elongation and membrane walker, C. D. R. D, Graham, J. T. Madison, E. E. Cary, and R. M. Welch, 1989, Effects of N: deficiency on some nitrogen metabolites in cowpeas (Vigan unguiculata L. Walp). Plant physiol. 79:474-479.
Woolhouse, H. W., 1989, Toxicity and tolerance in the responses of plants to metals, pp: 245-300. In: O. L. lange, P. S. Nobel, C. B.
Yang, X., Baligor, V. C., Mantens, D. C., Clark, R. B., 1996, plant tolerance to Nickel toxicity:I. Influx, transport, and accumulation of Nickel in four species, Journal of plant nutrition. 19(1), 73-85.
Chao, L., Xiong, Z-T., Geng, B., 2006, Phyrotoxic effects of copper on nitrogen metabolism and plant growth in Brassica pekinensis Rupr, Ecotoxicology and Environmental Safety. V:64. 273-280.
Craker, L.E., Xin Craker, L.E., Xing, B., Zheljaz kov, V.D., 2006, Effects of Cd, Pb, and Cu on growth and essential oil contents in dill, peppermint, and basil, Envir. & Exper. Botany., 58:9-16.
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قربانلی، م. (1383) فیزیولوژی گیاهی3، انتشارات دانشگاه پیام نور
Brigezu, K., Lichtenberger, O., Leopol, I., Neumann, D., 1999, Heavy metal tolerance of Silene vulgaris, J plant physiol, 158:536-546.
Badr-Alaoui, Patricia Genet, Florence Vinit Dunand, Marie-Laure Toussaint, Daniel Epron and Pierre-Marie Badot, 2003, Effect of copper on growth in cucumber plants (Cucumis sativus) and its relationships with carbohydrate accumulation and change in ion contents, Plant Science Vol., 166:1213-1218
Barcelo, Je., Poschenrieder, C., 1990, Plant water relations as affected by heavy metals: a review. J plant Nutr., 1:1-37.
Bates, L. S., Waldren, R. P., Treare, I. D., 1973, Rapid determination of free proline for water-stress studies, plant soil., 39-205-207.
Bewley, J. D., Black, M., 1994, Seeds: physiology of development and germination. Second edition. Plenum press, New York.
Clarkson, D. T. and Luttge, U., 1989, Divalent Cations, Transport and compartmentation, Prog. Bot., 51:93-112.
Defilippis, L. F., 1979, The effect of heavy metal compounds on the permeability of chlorella cells. Z. Pflanzenphysiol, 92-39-49.
Delong, J. M., Steffen, K. L., 1997, Photosynthetic function, lipid peroxiation and alpha tocopherol content in spinach leaves during exposure to UVB radiation, Canadian journal of plant science., 77:453-459.
Greger, M., Bertell, G., 1992, Effects of Ni2+ and Cd2+ on the carbohydrate metabolism in sugar beet (Beta vulgaris)., J Exp Bot., 43:467-173.
Guo, Z., Tan, H., Zhu, Z., Lu, S., 2005 Effect of intermediates on ascorbic acid and oxalate biosynthesis of rice and in relation to its stress resistance, plant physiol & Bioch. 43: 955-962.
Harberd, N. P., Peng, J., 2002, The role of GA-mediated signaling in the control of germination. Science. 5:376-381.
Hendry GAF, Baker AJM, Ewart CF, 1992, Heavy metal tolerance and toxicity, oxygen radical processes and molecular damage in Cadmium tolerant clones of Holcus lanatus L. Acta Bot Neerl., 41:271-281.
Hippeli, S., Elstner, E. F., 1996, Mechanism of oxygen activation during plant stress: Bio Chemical effect of air pollutants. Journal of Plant physiology., 148:249-257.
Ibarra-Caballero J, Villanueva- Verdu Zco J, Molina-Galam J, J imerez ES-D, 1988, Proline accumulation as a symptom of drought stress in maize: a tissue differentiation requirement. Journal of Experimental Btany., 89:889-897.
Kochert, G., 1978, Carbohydrate determination by the phenol sulfuric acid method, In Helebust, J. A., Craig, J. S(ed): Hand book of phycological Methods., pp: 96-97 carbridge unir. Press, Cambridge.
Fanimokan, V. O., Adedeji, F. O., 1984, Copper deficiency and toxicity in two tropical leaf vegetables (Celosia argentea L. and Amaranthus dubius mart. ex thell), Envir & Exper. Botany., 24:105-110.
Mehta, Sk., Cour, J. P., 1999, Heavy metal-induced proline accumulation and its role in ameliorating metal toxicity in Chlorella vulgaris., New phytol., 143:253-259.
Mishra, A., Choudhuri, MA., 1997, Differential effects of pb2+ and Hg2+ on inhibition of germination of seed of two cultivars. Indian Journal of plant physiology 2(1)
Moya, J. L., Ros, R., Picazo, I., 1993, Influence of Cademium and Nickel on growth, net photosynthesis and carbohydrate distribution in rice plants, Photosynthesis research., 36:75-80.
Naidu, C. V., Rajendrudu, G., Swamy, P. M., 2000, Effect of plant growth regulators on seed germination of Sapindus trifoliatus. Seed Science and Technology., 28:249-252.
Palanca, J., et al, 2000, The control of As synthesis and turn over in pea seedling. J. Exp. Botany., 345-674.
Pandey, N., Sharma, C. P., 2002, Effect of heavy metal Co2+ Ni2+ and Cd2+ on growth and metabolism of cabbage, Plant Sci, 163: 753-758.
Pandolfini, T., Gabbrielli R, Commparini, C., 1992, Nickel toxicity and peroxidase activity In seedings of Triticum aestivum L., Plant Cell Environ., 15:719-725
Poschenrieder, C. B., Gunse, Barcelo. J., 1989, Influence of heavy metals on water relations, stomatal resistance, and abscisic asid content in expanding bean leaves, plant physiol., 90:1365-1371.
Stearns, J. C., Shah, S., Greenberg, B. M., Oixon, D. G., Glick, B. R., 2005, Tolerance of transgenic canola expressing 1- amino cyclopropane- 1- Carboxylic acid deaminase to growth inhibition by nickel, Plant Physiology, Bichem, 41: 701-708.
Vincent, W. F., Roy, S., 1993, Solar ultraviolet-B radioation and aquatic primary production: damage, protection and recovery Environmental reviews 1:1-12.
|
Wain wright, S. J., Woolhouse, H. W., 1977, Some physiological Aspects of Nickel and Zinc tolerance in Agrostis tenuis: Cell Elongation and membrane walker, C. D. R. D, Graham, J. T. Madison, E. E. Cary, and R. M. Welch, 1989, Effects of N: deficiency on some nitrogen metabolites in cowpeas (Vigan unguiculata L. Walp). Plant physiol. 79:474-479.
Woolhouse, H. W., 1989, Toxicity and tolerance in the responses of plants to metals, pp: 245-300. In: O. L. lange, P. S. Nobel, C. B.
Yang, X., Baligor, V. C., Mantens, D. C., Clark, R. B., 1996, plant tolerance to Nickel toxicity:I. Influx, transport, and accumulation of Nickel in four species, Journal of plant nutrition. 19(1), 73-85.
Chao, L., Xiong, Z-T., Geng, B., 2006, Phyrotoxic effects of copper on nitrogen metabolism and plant growth in Brassica pekinensis Rupr, Ecotoxicology and Environmental Safety. V:64. 273-280.
Craker, L.E., Xin Craker, L.E., Xing, B., Zheljaz kov, V.D., 2006, Effects of Cd, Pb, and Cu on growth and essential oil contents in dill, peppermint, and basil, Envir. & Exper. Botany., 58:9-16.