Investigation on the effect of NaCl on the activity of peroxidase and peroxidation of lipids in roots of two sensitive and tolerant cultivars of wheat (Triticum aestivum L.)
Subject Areas : Geneticفائزه Ghanati, 1 , Elham Nayyeri Torshizi 2
1 - Dept. Plant Biol. Fac. Biol. Sci. Tarbiat Modares University, Tehran-Iran
2 - کارشناس ارشد فیزیولوژی گیاهی
Keywords: Salinity, Lipid peroxidation, Triticum aestivum, lignin, Wall bound proxidase, Soluble peroxidase,
Abstract :
In this paper, the effect of NaCl on the activity of soluble (SPO) and wall bound peroxidases (IPO, CPO) as well as the level of lipid peroxidation in roots of two cultivars of wheat (Triticum aestivum L.) were studied. Two wheat cultivars, Mahooti and Alamoot were selected as salt-tolerant and salt-sensitive cultivars, respectively. The plants were treated with 300mM NaCl for 24, 48 and 96 hours. The content of lignin and the ratio of CWP/FW were studied during 96 hours of treatment as well. Salt treatment increased the rate of lipid peroxidation and enhanced the activity of wall bound peroxidases, particularly in roots of Mahooti. These phenomena were also associated with an increase in the content of lignin in the walls. The activity of soluble peroxidase was also stimulated which helps to more scavenging of peroxide radicals produced by NaCl treatment. Meanwhile, the results suggest that in salt-tolerant wheat cultivar, NaCl treatment accelerated aging process.
_||_
Atak, C., Celik, O., Olgun, A., Alikamanoglu, S., Rzakoulieva, A., (2007). Effect of magnetic field on peroxidase activities of soybean tissue culture. Biotechnol. & Biotechnol. Eq. 21/2007/2.
Bradford, M.M., (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
DeVos, C.H.R., Schat, H., De Waal, M.A.D., (1991). Increased resistance to copper-induced damage of root plasma membranein copper tolerant cilene cucubalus. Physiol. Plant. 82: 523-528.
Elstner, E.F., (1987). Metabolism of activated oxygen species. In: Davies, D.D. (Ed.), The Biochemistry of Plants. vol. II, Biochemistry of Metabolism. Academic Press, San Diego, CA, pp. 252–315.
Fridovich, I., (1986). Biological effects of the superoxide radical. Arch. Biochem. Biophys. 247: 1–11.
Ghanati, F., Morita, A., Yokota, H., (2005). Effects of aluminum on the growth of tea plant and activation of antioxidant system. Plant and soil. 276: 133-141.
Heath, R. L., Packer, L., (1968). Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys.125: 189-198.
Iiyama, K., Wallis, A. F., (1990). Determination of lignin in herbaceous plants by an improved acetyle bromide procedure. J. Sci. Food Agric.51: 145-161.
Noctor, G., Foyer, C.H., (1998). Ascorbate and glutathione: keeping active oxygen under control. Annu. Rev. Plant Physiol. Plant Mol Biol. 49: 249–279.
Pandolfini, F., Gabbrielli, R., Comparini, C., (1992). Nickel toxicity and peroxidase activity in seedlings of Triticum aestivum L. Plant Cell Environ.15: 719-725.
Saroop, S., Chanda, S.V., Singh, Y.D., (2002). Changes in soluble and ionically bound peroxidase activities during Brassica juncea seed development.Bulg. J. Plant Physiol.28 (3-4): 26-34.
Schloss, P., Walter, C., Mader M., (1987). Basic peroxidases in isolated vacuoles of Nicotiana tabacum L. Planta 170: 225-229.
Valero, P., Nicolas, G., Labrador, E., (1991). Variations of cell wall peroxidases in epicotyls of Cicer arietinum during growth. Plant Sci., 74: 171-178.
Wakabayashi, K., Hoson, T., Kamisaka, S., (1997). Osmotic stress suppresses cell wall stiffening and the increase in cell wall-bound ferulic and diferulic acids in Wheat coleoptiles. Plant Physiol. 113: 967-973.