Study of Differential Responses of Anti Oxidative Enzymes of Two Sensitive and Tolerant Cultivars of Wheat (Triticum aestivum L.) to Salt Stress
Subject Areas : Genetic
فائزه
Ghanati,
1
(Dep. Biology. Tarbiat Modares University, Tehran, Iran)
الهام
Nayyeri Torshizi,
2
(Dep. Biology. Tarbiat Modares University, Tehran, Iran)
Keywords: Wheat, ascorbate peroxidase, Catalase, superoxide dismutase, Salt stress,
Abstract :
In this article the effects of salt stress on the activity of antioxidative anzymes and lipid peroxidation were studied in shoots and roots of two cultivars of wheat. The cultivar Mahooti and Alamoot were selected as salt-tolerant and salt-sensitive, respectively. The plants were treated for 24, 48, 96 hours. Mostly salt stress increased the activity of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) in treated plants. Also, the increase in level of CAT activity in roots was more pronounced in the salt-tolerant than in the salt-stress that is indicated the role of CAT for tolerance in Mahooti. The increase in activity of these enzymes during 24 hours of treatment with NaCl in salt-tolerant plants were more than in salt-sensitive plants. Lipid peroxidation had a little increase in treated plants, compared to those of the control ones. Salt stress had no significant effect on accumulation of Na+ in two cultivars. The results indicated that the oxidative stress play an important role in the activity of different enzymes in salt-stress and salt-tolerant plants. Also, rate of enzyme activities showed some flactuations, which suggest the cooperation of antioxidant enzymes with each other corresponding to different times and different stages of the growth.
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