Extraction and determination of flavonoid compounds kaempferol and quercetin in petals of Rosa damascene Mill. from North and North eastern Regions of Iran

Subject Areas : Genetic

Jaimand, جایمند ¹ , Rezaee, M.B ² , M.H Asareh ³ , S.R Tabaei Aghdaei ⁴ , سعیده Meshkyzadeh, ⁵

1 - . Department of Medicinal Plants, Research Institute of Forests and Rangelands, Tehran, Iran
2 - . Department of Medicinal Plants, Research Institute of Forests and Rangelands, Tehran, Iran
3 - Department of Biotechnology, Research Institute of Forests and Rangelands, Tehran, Iran
4 - Department of Biotechnology, Research Institute of Forests and Rangelands, Tehran, Iran
5 - Department of Medicinal Plants, Research Institute of Forests and Rangelands, Tehran, Iran

Received: 2008-03-07 Accepted : 2008-06-09 Published : 2008-12-21

Keywords: Flavonoids, Rosa damascena Mill, High Performance Liquid Chromatography (HPLC), Kaempferol, Quercetin glucoside,

Abstract :

Flavonoid components are the largest phenolic groups in nature. Kaempferol, quercetin has medicinal effects againts viruses and cancer cells. In f this research for extraction and determination of flavonoid compounds kaempferol and Quercetin in petals of Rosa damascena from north and north eastern of Iran, samples were collected in research Institute of Forest and Rangelands. Fresh flower petals were extracting and were analyzed by high performance liquid chromatography (HPLC). Two flavonoids kaempferol and quercetin glycoside compounds were extracted from 7 samples. The highest kaempferol content were obtained from Mazanderan 758ppm, Golestan 354ppm, and Cemnan 1, 349ppm, and the lowest from Gilan, 243ppm, Khorasan 2, 245ppm, and Khorasan 1, 283ppm. The highest quercetin glucoside contents were obtained from Khorasan 2, 2763ppm, Golestan, 617ppm, and Khorasan 1, 266ppm, and lowest were from Cemnan 1, 100 ppm, Gilan 174 ppm, and Mazanderan 204 ppm. According to the results best samples were from Khorasan 2 (245ppm kaempferol, 2763ppm quercetin), Golestan (354ppm kaempferol, 617ppm quercetin) and Khorasan 1 (283ppm kaempferol, 266 ppm quercetin).

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