Screening different cold-resistant sugarcane (Saccharum ssp. L) genotypes using morphological and biochemical indices
Subject Areas : Genetic
mahmoud
fouladvand
1
(Department of Biotechnology and Plant Breeding, Islamic Azad University, Science and Research Branch, Tehran, Iran)
asa
ebrahimi
2
(Department of Biotechnology and Plant Breeding, Islamic Azad University, Science and Research Branch, Tehran, Iran)
mehdi
rahaei
3
(Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Postal Code: 1439957131, Iran)
vahid
Shariati joni
4
(Department of Plant Molecular Biotechnology, National Research Institute of Genetic Engineering and Biotechnology, Tehran, Iran)
Keywords: Sugarcane, Cold stress, Morphological screening, Cold resistance, Biochemical index,
Abstract :
Sugarcane is cultivated in different parts of the world up to a latitude of about 32 degrees north and south. The resistance of this plant to cold is low. Considering the sensitivity of sugarcane to cold, in order to identify the pathways and genes by differential expression in sugarcane cultivars during cold stress and employ them in sugarcane plant breeding programs, 454 sugarcanes were investigated using morphological and biochemical indices, in the Research Farm of Khuzestan Sugarcane Research Institute following the incidence of -1.2 ℃ temperature in the region in December 2015. In the first stage, the cold-tolerant or sensitive cultivars were selected using morphological indices. In the second stage, biochemical indices such as proline and malondialdehyde were measured in the tolerant cultivars (selected at the first stage) after a cold stress period in 2016, which showed increases in comparison with susceptible cultivars. According to the data obtained at the two stages, two cultivars, namely BR00-01 and TUC66-107, were identified as the most tolerant and sensitive to cold, respectively. Based on the results of morphological studies, the cultivars with a higher tolerance to cold stress were also hose with higher proline and MDA levels in the biochemical study stage compared with the sensitive cultivars. There was also a high correlation between morphological traits and biochemical indicators in terms of cold resistance. Therefore, by measuring morphological and biochemical indices, it is possible to determine to a large extent the type of reaction of a sugarcane cultivar to cold stress before doing molecular analysis and spending huge sums of money, and to select superior clones when sifting sugarcane clones at different breeding stages.
A group of researchers (2010). Final report of the cold project in sugarcane, Sugarcane Research and Training Institute of Khuzestan.
Bates, L.S., Waldern, R.P and Tear, ID. (1973). Rapid determination of free proline for water stress studies. Plant Soil, 39: 205-207
Gururaj, Hansigi. (2001). "Sugarcane Agriculture", Translated by Bahram Mirashkari, Tabriz, Islamic Azad University, Tabriz Branch.
Mantri, N.L., Ford, R., Coram, T.E and Pang, E.C.K. (2007). Transcriptional profiling of chickpea genes, BMC Genomics Journal, 8:303-307
Patrick, F., Murilo, P., Florian, B., Daniel, Johnson and Rowan, S. (2014). Chilling and frost tolerance in Miscanthus and Saccharum genotypes bred for cool temperate climates. Journal of Experimental Botany, 13: 3749–3758
Park, J-W., Benatti, T.R., Marconi, T., Yu, Q., Solis-Gracia, N. and Mora, V. (2015). Cold Responsive Gene Expression Profiling of Sugarcane and Saccharum spontaneum with Functional Analysis of a Cold Inducible Saccharum Homolog of NOD26- Like Intrinsic Protein to Salt and Water Stress. PloS ONE 10(5): 125- 132
Kim, J.C., Lee, S.H., Cheong, Y.H., Yoo, C-M., Lee, S.I., Chun, H.J., Yun, D-J., Hong, J.C., Lee, S.Y., Lim, CO. (2001). A novel cold-inducible zinc finger protein from soybean, SCOF-1, enhances cold tolerance in transgenic plants. Plant Journal 25 : 247–259.
Rafaela, Queiroz., Maria, Durvalina., Sergio, Antonio., Domingues, Samira., Marcelo, Carlin. (2011). Biochemical and physiological responses of sugarcane cultivars to soil water deficiencies, Sci. Agric Journal. (Piracicaba, Braz.), 4: 469-476.
Robert, RC. and Bewlery, JD. (1980). Lipid peroxidation associated with accelerated aging of soybean axes. Plant Physiology Journal, 65: 245–248.
Teulate, B., Monneveux, P.J., Borrieres, C., Souyrus, I., Charri, A. and This, D. (1997). Relationshipes between relative water content and growth parameters and water stress in barley, a QTL study. New Physiology Journal, 137: 99-107.
Valentovic, P., M, Luxova., L, Kolarovi and O, Gasparikora. (2006). Effect of osmotic stress on compatible solutes content, memberane stability and water relation in two maizes. Plant Soil Environment Journal. 52 (4):186-191.
Heinz, Don J. (1987). Sugarcane Improvement through Breeding, Hawaiian sugar planters Association New York, Amsterdam, Elsevier Publications
Rahimizadeh, M., Habibi, D., Madani, H., Mohammadi, H., Mehraban, A. and Sabet, A.M. (2007). The effect of micronutrients on antioxidant enzymes metabolism in sunflower (Helianthus annuus L.) under drought stress. Helia Journal, 47: 167-174.
Reinhart, BJ., Weinstein, EG., Rhoades, MW., Bartel, B. and Bartel, DP. (2002). MicroRNAs in plants. Genes Dev 16: 1616–1626.
Li, B., Duan, H., Li, J., Deng, XW., Yin, W. and Xia, X. (2013). Global identification of miRNAs and targets in Populus euphratica under salt stress. Plant Mol Biol Journal, 81(6): 525–539.
Zhang, M.Q., Chen, RK and Lu, J.L. (1999). Effects of low temperature stress on the chlorophyll a fluorescence induction kinetics in the seedling of sugarcane. Fujian Agricultural University Journal, (Science & Technology Edition) 28(1): 1–7.
Jiang, M.Y., Guo, S.Q. and Zhang, X.M. (1997). Proline accumulation in rice seedlings exposed to oxidative stress in relation to autoxidation. Acta Phytophysiolica Sinica Journal, 23(4): 347–352.
Huang, SQ., Xiang, AL., Che, LL., Chen, S., Li, H., Song, JB. and Yang, ZM. (2010). A set of miRNAs from Brassica napus in response to sulphate deficiency and cadmium stress. Plant Biotechnol Journal, 8: 887–899.
Chen, L., Zhang, Y., Ren, Y., Xu, J., Zhang, Z., and Wang, Y. (2012). Genome-wide identification of cold-responsive and new microRNAs in Populus tomentosa by high-throughput sequencing. Biochem Biophys Res Commun Journal, 417: 892–896.
Zhou, SM., Kong, XZ., Kang, HH., Sun, X-D. and Wang, W. (2015). The Involvement of Wheat F-box Protein Gene TaFBA1 in the Oxidative Stress Tolerance of Plants. PLoS ONE Journal, 10(4): 117-127.
_||_
A group of researchers (2010). Final report of the cold project in sugarcane, Sugarcane Research and Training Institute of Khuzestan.
Bates, L.S., Waldern, R.P and Tear, ID. (1973). Rapid determination of free proline for water stress studies. Plant Soil, 39: 205-207
Gururaj, Hansigi. (2001). "Sugarcane Agriculture", Translated by Bahram Mirashkari, Tabriz, Islamic Azad University, Tabriz Branch.
Mantri, N.L., Ford, R., Coram, T.E and Pang, E.C.K. (2007). Transcriptional profiling of chickpea genes, BMC Genomics Journal, 8:303-307
Patrick, F., Murilo, P., Florian, B., Daniel, Johnson and Rowan, S. (2014). Chilling and frost tolerance in Miscanthus and Saccharum genotypes bred for cool temperate climates. Journal of Experimental Botany, 13: 3749–3758
Park, J-W., Benatti, T.R., Marconi, T., Yu, Q., Solis-Gracia, N. and Mora, V. (2015). Cold Responsive Gene Expression Profiling of Sugarcane and Saccharum spontaneum with Functional Analysis of a Cold Inducible Saccharum Homolog of NOD26- Like Intrinsic Protein to Salt and Water Stress. PloS ONE 10(5): 125- 132
Kim, J.C., Lee, S.H., Cheong, Y.H., Yoo, C-M., Lee, S.I., Chun, H.J., Yun, D-J., Hong, J.C., Lee, S.Y., Lim, CO. (2001). A novel cold-inducible zinc finger protein from soybean, SCOF-1, enhances cold tolerance in transgenic plants. Plant Journal 25 : 247–259.
Rafaela, Queiroz., Maria, Durvalina., Sergio, Antonio., Domingues, Samira., Marcelo, Carlin. (2011). Biochemical and physiological responses of sugarcane cultivars to soil water deficiencies, Sci. Agric Journal. (Piracicaba, Braz.), 4: 469-476.
Robert, RC. and Bewlery, JD. (1980). Lipid peroxidation associated with accelerated aging of soybean axes. Plant Physiology Journal, 65: 245–248.
Teulate, B., Monneveux, P.J., Borrieres, C., Souyrus, I., Charri, A. and This, D. (1997). Relationshipes between relative water content and growth parameters and water stress in barley, a QTL study. New Physiology Journal, 137: 99-107.
Valentovic, P., M, Luxova., L, Kolarovi and O, Gasparikora. (2006). Effect of osmotic stress on compatible solutes content, memberane stability and water relation in two maizes. Plant Soil Environment Journal. 52 (4):186-191.
Heinz, Don J. (1987). Sugarcane Improvement through Breeding, Hawaiian sugar planters Association New York, Amsterdam, Elsevier Publications
Rahimizadeh, M., Habibi, D., Madani, H., Mohammadi, H., Mehraban, A. and Sabet, A.M. (2007). The effect of micronutrients on antioxidant enzymes metabolism in sunflower (Helianthus annuus L.) under drought stress. Helia Journal, 47: 167-174.
Reinhart, BJ., Weinstein, EG., Rhoades, MW., Bartel, B. and Bartel, DP. (2002). MicroRNAs in plants. Genes Dev 16: 1616–1626.
Li, B., Duan, H., Li, J., Deng, XW., Yin, W. and Xia, X. (2013). Global identification of miRNAs and targets in Populus euphratica under salt stress. Plant Mol Biol Journal, 81(6): 525–539.
Zhang, M.Q., Chen, RK and Lu, J.L. (1999). Effects of low temperature stress on the chlorophyll a fluorescence induction kinetics in the seedling of sugarcane. Fujian Agricultural University Journal, (Science & Technology Edition) 28(1): 1–7.
Jiang, M.Y., Guo, S.Q. and Zhang, X.M. (1997). Proline accumulation in rice seedlings exposed to oxidative stress in relation to autoxidation. Acta Phytophysiolica Sinica Journal, 23(4): 347–352.
Huang, SQ., Xiang, AL., Che, LL., Chen, S., Li, H., Song, JB. and Yang, ZM. (2010). A set of miRNAs from Brassica napus in response to sulphate deficiency and cadmium stress. Plant Biotechnol Journal, 8: 887–899.
Chen, L., Zhang, Y., Ren, Y., Xu, J., Zhang, Z., and Wang, Y. (2012). Genome-wide identification of cold-responsive and new microRNAs in Populus tomentosa by high-throughput sequencing. Biochem Biophys Res Commun Journal, 417: 892–896.
Zhou, SM., Kong, XZ., Kang, HH., Sun, X-D. and Wang, W. (2015). The Involvement of Wheat F-box Protein Gene TaFBA1 in the Oxidative Stress Tolerance of Plants. PLoS ONE Journal, 10(4): 117-127.