Investigation the role of nitric oxide on drought tolerance of mung bean (Vigna radiata L.)
Subject Areas : Genetic
1 - Department of Agronomy, Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran
Keywords: Proline, foliar application, Antioxidant enzymes, seed treatment, Sodium nitroprusside,
Abstract :
Drought is one of the most important adverse environmental factors that limits crop production. Nitric oxide (NO) as a signaling molecule contributes in plant response to environmental stresses. Therefore, in order to investigate the effects of NO on drought tolerance of mung bean (cv. Partow), a field experiment was done in the south of Tehran in 2015. The study was conducted as a randomized complete block design with 9 treatments and 4 replications. In this experiment, sodium nitroprusside (SNP) solution was used as NO donor. Treatments included control, drought stress, seed treatment, foliar application at the vegetative stage, foliar application at the reproductive stage, seed treatment + foliar application at the vegetative stage, seed treatment + foliar application at the reproductive stage, foliar application at the vegetative and reproductive stages, and seed treatment + foliar application at the vegetative and reproductive stages. Results showed that due to the drought stress, antioxidant enzymes activities such as superoxide dismutase, catalase, and ascorbate peroxidase, as well as malondialdehyde (MDA) and proline concentrations were increased but the relative water content (RWC), chlorophyll value, leaf area index (LAI), and seed yield decreased. However, application of SNP by further increasing the activity of antioxidant enzymes and proline accumulation, reduced the MDA content while improving the RWC, chlorophyll value, LAI, and eventually the seed yield under drought stress. Among SNP application treatments, seed treatment + foliar application at the vegetative and reproductive stages was the most effective, although it was not significantly difference from the other treatments including SNP foliar application at the reproductive stage. According to the findings of the study, application of NO may be recommended as a useful tool for improving drought tolerance of mung bean.
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Aebi, H. (1984). Catalase in vitro. Methods in Enzymology. 105: 121-126.
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Beyer, W.F. and Fridovich, I. (1987). Assaying for superoxide dismutase activity: Some large consequences of minor changes in conditions. Analytical Biochemistry. 161(2): 559-566.
Cechin, I., Cardoso, G.S., Fumis, T.F. and Corniani, N. (2015). Nitric oxide reduces oxidative damage induced by water stress in sunflower plants. Bragantia Campinas. 74(2): 200-206.
Fan, H., Li, T., Guan, L., Li, Z., Guo, N., Cai, Y. and Lin, Y. (2012). Effects of exogenous nitric oxide on antioxidation and DNA methylation of Dendrobium huoshanense grown under drought stress. Plant Cell, Tissue and Organ Culture. 109: 307-314.
Fan, Q. and Liu, J. (2012). Nitric oxide is involved in dehydration/drought tolerance in Poncirus trifoliata seedlings through regulation of antioxidant systems and stomatal response. Plant Cell Reports. 31: 145-154.
Farooq, M., Basra, S.M.A., Wahid, A. and Rehman, H. (2009). Exogenously applied nitric oxide enhances the drought tolerance in fine grain aromatic rice (Oryza sativa L.). Journal of Agronomy & Crop Science. 195: 254-261.
Gan, L., Wu, X. and Zhong, Y. (2015). Exogenously applied nitric oxide enhances the drought tolerance in hulless barley. Plant Production Science. 18(1): 52-56.
Gill, S.S. and Tuteja, N. (2010). Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology & Biochemistry. 48: 909-930.
Heath, R.L. and Packer, L. (1968).Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry & Biophysics. 125: 189-198.
Jangid, K.K. and Dwivedi, P. (2017). Physiological and biochemical changes by nitric oxide and brassinosteroid in tomato (Lycopersicon esculentum Mill.) under drought stress. Acta Physiologea Plantarum. 39: 73.
Khan, M.N., Mobin, M., Mohammad, F. and Corpas, F.J. (2014). Nitric oxide in plants: Metabolism and role in stress physiology (1st ed.). Springer Science.
Kumari, A., Sheokand, S. and Swaraj, K. (2010). Nitric oxide induced alleviation of toxic effects of short term and long term Cd stress on growth, oxidative metabolism and Cd accumulation in chickpea. Brazilian Journal of Plant Physiology. 22: 271-284.
Li, C., Song, Y., Guo, L., Gu, X., Muminov, M.A. and Wang, T. (2018). Nitric oxide alleviates wheat yield reduction by protecting photosynthetic system from oxidation of ozone pollution. Environmental Pollution. 236: 296-303.
Mofizur Rahman, I. and Hasegawa, H. (2012). Water stress (1st ed.). Intech Publisher.
Nakano, Y. and Asada, K. (1981). Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant and Cell Physiology. 22(5): 867-880.
Rahimian Boogar, A., Salehi, H. and Jowkar, A. (2014). Exogenous nitric oxide alleviates oxidative damage in turfgrasses under drought stress. South African Journal of Botany. 92: 78-82.
Santisree, P., Bhatnagar-Mathur, P. and Sharma, K.K. (2015). NO to drought-multifunctional role of nitric oxide in plant drought: Do we have all the answers? Plant Science. 239: 44-55.
Saroj, S., Dahire, A., Dewangan, M. and Jain, A. (2018). Assessment the effect of nitric oxide on yield parameters of wheat and maize under different levels of salt stress. International Journal of Current Microbiology & Applied Sciences. 7(1): 1835-1842.
Seabra, A.B. and Oliveira, H.C. (2016). How nitric oxide donors can protect plants in a changing environment: What we know so far and perspectives. Molecular Science. 3(4): 692-718.
Shallan, M.A., Hassan, H.M.M., Namich, A.A.M. and Ibrahim, A.A. (2012). Effect of sodium niroprusside, putrescine and glycine betaine on alleviation of drought stress in cotton plant. American-Eurasian journal of agricultural & environmental sciences. 12(9): 1252-1265.
Shehab, G.G., Ahmed, O.K. and EL-Beltagi, H.S. (2010). Effects of various chemical agents for alleviation of drought stress in rice plants (Oryza sativa L.). Notulae Botanicae Horti Agrobotanici Cluj-Napoca.38(1): 139-148.
Sidana, S., Bose, J., Shabala, L. and Shabala, S. (2015). Nitric oxide in drought stress signalling and tolerance in plants. In: Nitric oxide action in abiotic stress responses in plants, pp. 95-114. Ed. M.N. Khan, M. Mobin, F. Mohammad and F.J. Corpas. Springer Science.
Silveira, N.M., Hancock, J.T., Frungillo, L., Siasou, E., Marcos, F.C.C., Salgado, I., Machado, E.C. and Ribeiro, R.V. (2017). Evidence towards the involvement of nitric oxide in drought tolerance of sugarcane. Plant Physiology & Biochemistry. 115: 354-359.
Tan, J., Zhao, H., Hong, J., Han, Y., Li, H. and Zhao, W. (2008). Effects of exogenous nitric oxide on photosynthesis, antioxidant capacity and proline accumulation in wheat seedlings subjected to osmotic stress. World Journal of Agricultural Sciences. 4(3): 307-313.
Zangani, E., Zehtab-Salmasi, S., Andalibi, B. and Zamani, A.A. (2018). Protective effects of nitric oxide on photosynthetic stability and performance of Silybum marianum under water deficit conditions. Agronomy Journal. 110(2): 555-564.
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Adimulam, S.S., Pooja, B.M. and Parankusam, S. (2017). Interaction of nitric oxide with phytohormones under drought stress. Journal of Plant Studies. 6(1): 58-61.
Aebi, H. (1984). Catalase in vitro. Methods in Enzymology. 105: 121-126.
Bates, L.S., Waldren, R.P. and Teare, J.D. (1973). Rapid determination of proline for water stress studies. Plant and Soil. 39: 205-207.
Beyer, W.F. and Fridovich, I. (1987). Assaying for superoxide dismutase activity: Some large consequences of minor changes in conditions. Analytical Biochemistry. 161(2): 559-566.
Cechin, I., Cardoso, G.S., Fumis, T.F. and Corniani, N. (2015). Nitric oxide reduces oxidative damage induced by water stress in sunflower plants. Bragantia Campinas. 74(2): 200-206.
Fan, H., Li, T., Guan, L., Li, Z., Guo, N., Cai, Y. and Lin, Y. (2012). Effects of exogenous nitric oxide on antioxidation and DNA methylation of Dendrobium huoshanense grown under drought stress. Plant Cell, Tissue and Organ Culture. 109: 307-314.
Fan, Q. and Liu, J. (2012). Nitric oxide is involved in dehydration/drought tolerance in Poncirus trifoliata seedlings through regulation of antioxidant systems and stomatal response. Plant Cell Reports. 31: 145-154.
Farooq, M., Basra, S.M.A., Wahid, A. and Rehman, H. (2009). Exogenously applied nitric oxide enhances the drought tolerance in fine grain aromatic rice (Oryza sativa L.). Journal of Agronomy & Crop Science. 195: 254-261.
Gan, L., Wu, X. and Zhong, Y. (2015). Exogenously applied nitric oxide enhances the drought tolerance in hulless barley. Plant Production Science. 18(1): 52-56.
Gill, S.S. and Tuteja, N. (2010). Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology & Biochemistry. 48: 909-930.
Heath, R.L. and Packer, L. (1968).Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry & Biophysics. 125: 189-198.
Jangid, K.K. and Dwivedi, P. (2017). Physiological and biochemical changes by nitric oxide and brassinosteroid in tomato (Lycopersicon esculentum Mill.) under drought stress. Acta Physiologea Plantarum. 39: 73.
Khan, M.N., Mobin, M., Mohammad, F. and Corpas, F.J. (2014). Nitric oxide in plants: Metabolism and role in stress physiology (1st ed.). Springer Science.
Kumari, A., Sheokand, S. and Swaraj, K. (2010). Nitric oxide induced alleviation of toxic effects of short term and long term Cd stress on growth, oxidative metabolism and Cd accumulation in chickpea. Brazilian Journal of Plant Physiology. 22: 271-284.
Li, C., Song, Y., Guo, L., Gu, X., Muminov, M.A. and Wang, T. (2018). Nitric oxide alleviates wheat yield reduction by protecting photosynthetic system from oxidation of ozone pollution. Environmental Pollution. 236: 296-303.
Mofizur Rahman, I. and Hasegawa, H. (2012). Water stress (1st ed.). Intech Publisher.
Nakano, Y. and Asada, K. (1981). Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant and Cell Physiology. 22(5): 867-880.
Rahimian Boogar, A., Salehi, H. and Jowkar, A. (2014). Exogenous nitric oxide alleviates oxidative damage in turfgrasses under drought stress. South African Journal of Botany. 92: 78-82.
Santisree, P., Bhatnagar-Mathur, P. and Sharma, K.K. (2015). NO to drought-multifunctional role of nitric oxide in plant drought: Do we have all the answers? Plant Science. 239: 44-55.
Saroj, S., Dahire, A., Dewangan, M. and Jain, A. (2018). Assessment the effect of nitric oxide on yield parameters of wheat and maize under different levels of salt stress. International Journal of Current Microbiology & Applied Sciences. 7(1): 1835-1842.
Seabra, A.B. and Oliveira, H.C. (2016). How nitric oxide donors can protect plants in a changing environment: What we know so far and perspectives. Molecular Science. 3(4): 692-718.
Shallan, M.A., Hassan, H.M.M., Namich, A.A.M. and Ibrahim, A.A. (2012). Effect of sodium niroprusside, putrescine and glycine betaine on alleviation of drought stress in cotton plant. American-Eurasian journal of agricultural & environmental sciences. 12(9): 1252-1265.
Shehab, G.G., Ahmed, O.K. and EL-Beltagi, H.S. (2010). Effects of various chemical agents for alleviation of drought stress in rice plants (Oryza sativa L.). Notulae Botanicae Horti Agrobotanici Cluj-Napoca.38(1): 139-148.
Sidana, S., Bose, J., Shabala, L. and Shabala, S. (2015). Nitric oxide in drought stress signalling and tolerance in plants. In: Nitric oxide action in abiotic stress responses in plants, pp. 95-114. Ed. M.N. Khan, M. Mobin, F. Mohammad and F.J. Corpas. Springer Science.
Silveira, N.M., Hancock, J.T., Frungillo, L., Siasou, E., Marcos, F.C.C., Salgado, I., Machado, E.C. and Ribeiro, R.V. (2017). Evidence towards the involvement of nitric oxide in drought tolerance of sugarcane. Plant Physiology & Biochemistry. 115: 354-359.
Tan, J., Zhao, H., Hong, J., Han, Y., Li, H. and Zhao, W. (2008). Effects of exogenous nitric oxide on photosynthesis, antioxidant capacity and proline accumulation in wheat seedlings subjected to osmotic stress. World Journal of Agricultural Sciences. 4(3): 307-313.
Zangani, E., Zehtab-Salmasi, S., Andalibi, B. and Zamani, A.A. (2018). Protective effects of nitric oxide on photosynthetic stability and performance of Silybum marianum under water deficit conditions. Agronomy Journal. 110(2): 555-564.