Morpho-physiological and yield responses of cumin (Cuminum cyminum L.) to the application of growth regulators under drought stress

Subject Areas : Stress Physiology

Habib  Noori ¹ (Ph.D. student, Faculty of Agriculture, Birjand Branch, Islamic Azad University, Birjand, Iran)
Seyyed Gholamreza  Moosavi ² (Associate Professor, Agricultural, Medicinal Plants and Animal Sciences Research Center, Birjand branch, Islamic Azad University, Birjand, Iran)
Mohammadjavad  Seghatoleslami ³ (Associate Professor, Faculty of Agriculture, Birjand Branch, Islamic Azad University, Birjand, Iran)
Mansour  Fazeli Rostampour ⁴ (Horticultural Crops Research Department, Sistan Agricultural and Natural Resources Research and Education Center, AREEO, Zabol, Iran|Department of Agriculture, Birjand Branch, Islamic Azad University, Birjand, Iran)

Received: 2022-02-02 Accepted : 2022-02-09 Published : 2022-01-01

Keywords: Drought stress, paclobutrazol, Growth Regulator, photosynthetic pigment, Antioxidant enzymes,

Abstract :

Cumin (Cuminum cyminum L.) is an industrial plant whose quality is affected by foliar application and irrigation regimes. In the present study, a field experiment was designed to assess the responses of cumin to foliar nutrition with salicylic acid (SA), jasmonic acid (JA), paclobutrazol (PBZ), and chitosan (CS) treatments under drought stress in Sistan Agricultural and Natural Resources Research and Education Center during 2019-2020. This experiment was performed as split plots based on randomized complete block design with three replications. The irrigation regime included three levels according to the evapotranspiration percentage of reference plant (supply of 100%, 70% and 40% water requirement of the plant (WR)). Based on the results, drought stress negatively affected photosynthetic pigments, yield, and yield components. Morpho-physiological traits improved under drought stress and non-stress conditions following the foliar fertilization. Moreover, foliar nutrition, especially JA, PBZ, and SA, resulted in synthesizing more photosynthetic pigments and proline, rising antioxidant enzyme activity, and consequently increasing yield components. The most positive effect of increasing chlorophyll content was related to the use of JA, which resulted in 27.9, 29.4, and 28.4% increase in chlorophyll a, b, and total, respectively. The highest SOD activity (3.89 U/mg protein) was related to the without foliar application under non-stress treatment. Additionally, SA, JA, and CS applications under severe water stress showed the highest SOD activity. The effect of SA and JA was superior on RWC. PBZ application under non-stress conditions showed the highest seed yield (698.9 kg/ha).

References:

Afshari, F., F. Nakhaei, S. Mosavi, and M. Seghatoleslami.2020. Evaluating the role of nutri-priming in improving PEG-induced drought stress tolerance of stevia (Stevia rebuadiana Bertoni). Iranian Journal of Plant Physiology, 11,3509-3522.

Aghighi Shahverdi, M., H. Omidi, and SJ. Tabatabaei. 2017. Effect of nutri-priming on germination indices and physiological characteristics of stevia seedling under salinity stress. Journal of Seed Science, 39,353-362.

Ahmad, MA.,R. Javed, M. Adeel, M. Rizwan, and Y. Yang. 2020. PEG 6000-stimulated drought stress improves the attributes of in vitro growth, steviol glycosides production, and antioxidant activities in Stevia rebaudiana Bertoni. Plants, 9,1552.

Alam, MM., K. Nahar, M. Hasanuzzaman, and M. Fujita. 2014. Exogenous jasmonic acid modulates the physiology, antioxidant defense and glyoxalase systems in imparting drought stress tolerance in different Brassica species. Plant Biotechnology Reports, 8,279-293.

Alinian, S., J. Razmjoo, and H. Zeinali. 2016. Flavonoids, anthocynins, phenolics and essential oil produced in cumin (Cuminum cyminum L.) accessions under different irrigation regimes. Industrial Crops and Products, 81,49-55.

Aly, AA., and H. H. Latif. 2011. Differential effects of paclobutrazol on water stress alleviation through electrolyte leakage, phytohormones, reduced glutathione and lipid peroxidation in some wheat genotypes (Triticum aestivum L.) grown in-vitro. Rom Biotech Lett, 6: 6710-6721.

Archangi, A., B. Heidari, and G. Mohammadi-Nejad. 2019. Association between seed yield-related traits and cDNA-AFLP markers in cumin (Cuminum cyminum) under drought and irrigation regimes. Industrial Crops and Products, 133,276-283.

Bayati, P., H. Karimmojeni, and J. Razmjoo. 2020. Changes in essential oil yield and fatty acid contents in black cumin (Nigella sativa L.) genotypes in response to drought stress. Industrial Crops and Products, 155,112764.

Beauchamp, C., and I. Fridovich. 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical biochemistry, 44:276-287.

Chance, B. 1955. Assay of catalase and peroxidase. Methods in enzymology, 2:765-775.

Cheong, J-J., and Y. Do Choi. 2003. Methyl jasmonate as a vital substance in plants. TRENDS in Genetics, 19,409-413.

Fathi, A., and D. B. Tari. 2016. Effect of drought stress and its mechanism in plants. International Journal of Life Sciences, 10,1-6.

Ghaffari, H., M. Tadayon, J. Razmjoo, M. Bahador, H. K. Soureshjani, and T.Yuan. 2020. Impact of jasmonic acid on sugar yield and physiological traits of sugar beet in response to water deficit regimes: Using Stepwise Regression Approach. Russian Journal of Plant Physiology, 67,482-493.

Ghasemi Pirbalouti, A., 2010. Medicinal and Aromatic Plants: Introduction and Application. IAU Press, Shahrekord. Iran

Ghahremani, A., E. G. Moghadam, M. Tatari, and S. Khosroyar. 2019. Physiological Responses of Paneer-Booti (Withania coagulans Dunal) to NaCl Stress Under Tissue Culture Conditions. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47,1365-1373.

Ghassemi-Golezani, K. and S. Farhangi-Abriz. 2018. Foliar sprays of salicylic acid and jasmonic acid stimulate H+-ATPase activity of tonoplast, nutrient uptake and salt tolerance of soybean. Ecotoxicology and Environmental Safety, 166,18-25.

Gorzi, A., H. Omidi, and A. Bostani. 2017. Morpho-physiological responses of Stevia (Stevia rebaudiana Bertoni) to various priming treatments under drought stress. Applied Ecology and Environmental Research, 16,4753-4771.

Habibi, G. 2012. Exogenous salicylic acid alleviates oxidative damage of barley plants under drought stress. Acta Biol Szeged, 56(1): 57-63

Hajihashemi, S., and A. A. Ehsanpour. 2013. Influence of exogenously applied paclobutrazol on some physiological traits and growth of Stevia rebaudiana under in vitro drought stress. Biologia, 68,414-420.

Hasanuzzaman, M.,M. A.  Matin, J. Fardus, M. Hasanuzzaman, MS. Hossain, and K. Parvin. 2019. Foliar application of salicylic acid improves growth and yield attributes by upregulating the antioxidant defense system in Brassica campestris plants grown in lead-amended soils. Acta Agrobotanica, 72(2).

Hayat, S., S. A. Hasan Q. Fariduddin and A. Ahmad. 2008. Growth of tomato (Lycopersicon esculentum) in response to salicylic acid under water stress. J Plant Interact, 3: 297-304.

Howell, T., S. Evett, J. Tolk, K. Copeland, P. Colaizzi, and P. Gowda. 2008. Evapotranspiration of corn and forage sorghum for silage. In World Environmental and Water Resources Congress: Ahupua'A, 1-14.

Hazrati, S., Z. Tahmasebi-Sarvestani, SAM. Modarres-Sanavy, A. Mokhtassi-Bidgoli, and S. Nicola. 2016. Effects of water stress and light intensity on chlorophyll fluorescence parameters and pigments of Aloe vera L. Plant Physiology and Biochemistry, 106,141-148.

Kadioglu, A., N. Saruhan, A. Sağlam, R. Terzi and T. Acet. 2011. Exogenous salicylic acid alleviates effects of long term drought stress and delays leaf rolling by inducing antioxidant system. Plant Growth Regul, 64: 27-37

Kang, DJ., Y. J. Seo, J. D. Lee, R. Ishii, K. Kim, D. Shin, S. Park, S. Jang, and IJ Lee. 2005. Jasmonic acid differentially affects growth, ion uptake and abscisic acid concentration in salt‐tolerant and salt‐sensitive rice cultivars. Journal of Agronomy and Crop Science, 191,273-282.

Lee, Y. S., Y. H. Kim and S. B. Kim. 2005. Changes in the respiration, growth, and vitamin C content of soybean sprouts in response to chitosan of different molecular weights. Horticulture Science, 40: 1333-1335.

Liu, C., J. Guo, Y. Cui, T. Lü, X. Zhang and G. Shi. 2011. Effects of cadmium and salicylic acid on growth, spectral reflectance and photosynthesis of castor bean seedlings. Plant Soil, 344: 131-141.

MacAdam, JW., C. J. Nelson, R.E. and Sharp. 1992. Peroxidase activity in the leaf elongation zone of tall fescue: I. Spatial distribution of ionically bound peroxidase activity in genotypes differing in length of the elongation zone. Plant Physiology, 99,872-878.

Mandal, S. 2010. Induction of phenolics, lignin and key defence enzymes in eggplant (Solanum melongena L.) roots in response to elicitors. African Journal of Biotechnology, 9: 8038-8047.

Qiu, Z., J. Guo, A. Zhu, L. Zhang, and M. Zhang. 2014. Exogenous jasmonic acid can enhance tolerance of wheat seedlings to salt stress. Ecotoxicology and Environmental Safety, 104,202-208.

Razmjoo, J., and S. Alinian. 2017. Influence of magneto priming on germination, growth, physiology, oil and essential contents of cumin (Cuminum cyminum L.). Electromagnetic biology and medicine, 36,325-329.

Reyhani, N., and I. Khashei Siuki. 2015. Lysimetric determination of cumin crop coefficients during different growth stages in region of Birjand. Journal of Water and Soil, 29,1047-1056.

Shahrokhi, M., A. Tehranifar, H. Hadizadeh, and  Y. Selahvarzi. 2011. Effect of drought stress and paclobutrazol-treated seeds on physiological response of Festuca arundinacea L. Master and Lolium perenne L. Barrage. J Biol Environ Sci, 5,77-85.

Shahverdi, MA., H. Omidi, and C.A. Damalas. 2020. Foliar fertilization with micronutrients improves Stevia rebaudiana tolerance to salinity stress by improving root characteristics. Brazilian Journal of Botany,1-11.

Shahverdi, MA., H. Omidi, and SJ.Tabatabaei. 2019. Stevia (Stevia rebaudiana Bertoni) responses to NaCl stress: Growth, photosynthetic pigments, diterpene glycosides and ion content in root and shoot. Journal of the Saudi Society of Agricultural Sciences, 18,355-360.

Shams Peykani, L. and M. Farzami Sepehr. 2018. Effect of chitosan on antioxidant enzyme activity, proline, and malondialdehyde content in Triticum aestivum L. and Zea maize L. under salt stress condition. Iranian Journal of Plant Physiology 9 (1), 2661- 2670.

Sheteiwy, MS.,H. Shao, W. Qi, P. Daly, A. Sharma, H. Shaghaleh, YA Hamoud, MA  El‐Esawi, R. Pan, and Q. Wan. 2020. Seed priming and foliar application with jasmonic acid enhance salinity stress tolerance of soybean (Glycine max L.) seedlings. Journal of the Science of Food and Agriculture. 101(5):2027-2041.

Soumya, P., P. Kumar, and M. Pal. 2017. Paclobutrazol: a novel plant growth regulator and multi-stress ameliorant. Indian Journal of Plant Physiology, 22,267-278.

Thakur, M., and R. Kumar. 2020. Foliar application of plant growth regulators modulates the productivity and chemical profile of damask rose (Rosa damascena Mill.) under mid hill conditions of the western Himalaya. Industrial Crops and Products, 158,113024.

Wang, L. J., L. Fan, W. Loescher, W. Duan, G. J. Liu and J. S. Cheng. 2010. Salicylic acid alleviates decreases in photosynthesis under heat stress and accelerates recovery in grapevine leaves. BMC Plant Biol, 10: 34-40.

Zafari, M., A. Ebadi, S. Jahanbakhsh, and M. Sedghi. 2020. Safflower (Carthamus tinctorius) biochemical properties, yield and oil content affected by 24-epibrassinosteroid and genotype under drought stress. Journal of Agricultural and Food Chemistry, 68(22) :6040-6047.

Zarrinabadi, IG., J. Razmjoo, A.A. Mashhadi, and A. Boroomand. 2019. Physiological response and productivity of pot marigold (Calendula officinalis) genotypes under water deficit. Industrial Crops and Products, 139,111488.

Zayed M. M., S. H. Elkafafi, A. M. G. Zedan and S. F. M. Dawoud. 2017. Effect of nano chitosan on growth, physiological and biochemical parameters of Phaseolus vulgaris under salt stress. Journal of Plant Production, 8 (5): 577- 585.