Influence of ascorbic acid on growth and micropropagation of Aloe barbadensis Mill.
Subject Areas : Plant PhysiologyAki Ramezani Sayad 1 , Ahmad Majd 2 , Mojtaba Khorrami Raad 3 , Behzad Kaviani 4
1 - Department of Tissue Culture, North Region Branch of Iran (Rasht), Agricultural Biotechnology Research Institute of Iran (ABRII)
2 - Department of Biology, Faculty of Sciences, Tarbiat-e-Moallem University, Tehran, Iran
3 - School of Ecosystem and Forest Sciences, Faculty of Science, The University of Melbourne, Creswick, Australia
4 - Department of Horticultural Science, Faculty of Agricultural Science, Islamic Azad University, Rasht, Iran
Keywords: Medicinal Plants, tissue culture, plant growth regulators, Vegetative growth, Aloe,
Abstract :
Ascorbic acid (AsA) plays various roles in plant metabolism. This study was carried out to evaluate the effect of different concentrations of AsA on the growth and micropropagation of Aloe barbadensis Mill., an important medicinal and ornamental herb, for the first time. In this regard, results obtained from applying different concentrations of AsA on variables such as aerial part length, root length and number, number of propagules, medium browning, and the fresh and dry weights of plants were analysed after 8 weeks. Control plants showed slower growth in the aerial parts compared to plants treated with AsA. Additionally, the leaves were smaller in the control plants. The fresh and dry weights of the aerial parts were less in the control plants than in those treated with AsA. There was a significant increase in the number of propagules produced in the different treatments compared to the control treatment. The average number and length of roots produced in plants treated with AsA were higher than those produced in the control plants. Furthermore, browning of the medium and tissue cultures was reduced in plants treated with AsA due to the presence of different phenolic compounds in these plants. Overall, AsA at a concentration of 80 mg L-1 had the greatest effect on the induction of growth and development of A. barbadensis Mill. in vitro.
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