A Study on the effect of moist-chilling and GA3 application on evening primrose (Oenothera biennis L.) seed germination

Subject Areas : Plant Physiology

Bakhtyar  Rezaee ¹ (Department of Horticulture, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Iran)
Azim  Ghasemnezhad ² (Department of Horticulture, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Iran)
Ebrahim  Zeinali ³ (Department of Horticulture, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Iran)

Received: 2019-11-04 Accepted : 2019-11-04 Published : 2019-08-01

Keywords: germination, Seed, GA3, moist-chilling, evening primrose,

Abstract :

Evening primrose (Oenothera biennis L.) is native to North America, belongs to Onagraceae family, and is a biennial plant. This experiment was performed as factorial based on a completely randomized design in three replications. The main purpose of the study was to evaluate the effect of moist-chilling and GA3 on seed germination and flower induction of evening primrose. The seeds were soaked in distilled water for 30 min, and treated with seven levels of moist-chilling period (0,10, 20, 30, 40, 50, and 60 days) and were kept in 2-4 °C in refrigerator conditions. At the end of day 60 half of the seeds were treated with 500 ppm GA3 solution for 24 hours. After that, both groups were planted in pots with three replications to see the plant reproductive responses. Simultaneously, the same seeds were planted in Petri dishes with three replications for seed germination. Germination was controlled every three days and seed germination test was done for 15 days. Seedling characteristics were measured at the end of germination test. Results showed that moist-chilling and GA3 significantly influenced the seed germination percentage. The highest germination percentage was observed in a combination of 20 days moist-chilling conditions and 500 ppm GA3. Rootlet length increased while moist-chilling was increased from 10 to 30 days. By increasing moist-chilling to 40 - 60 days, rootlet length decreased unexpectedly. Combination of moist-chilling and GA3 did not influence flower stem production and seed yield significantly. On the other hand, the earliest flower stem production was observed in seeds treated with moist chilling for 40 and 60 days. To guaranty flower stem production of spring sowing evening primrose in the areas with warm winter, seeds are strongly recommended to be treated with moist chilling for 40 days at 4 °C.

References:

Akhtar, N., M. Ibrar and N. Aman. 2008. 'The effects of different soaking times and concentrations of GA₃ on seed germination and growth of Spinacia oleracea L'. Pakistan. Journal of. Plant Sciences, 14 (1): 9-13.

Amooaghaie, R. 2009. 'The Effect Mechanism of Moist-Chilling and GA₃ on Seed Germination and Subsequent Seedling Growth of Feulaovina Boiss'. The Open Plant Science Journal, 3: 22-28.

Barpete, S., M.C.Oguz, S. F. Ozcan, E. Anayol, H. A.  Ahmed, K. M. Khawar and S. Ozcan. 2015. 'Effect of temperature on germination, seed vigor index and seedling growth of five Turkish cotton (Gossypium hirsutum L.) cultivars'. Fresenius Environ Bull, 24:2561-2566.

 Blumenthal, M., T. Hall, A. Goldberg, T. Kunz, K. Dinda, J. Brinckmann and B. Wollschlaeger.2003. The ABC Clinical Guide to Herbs. 2003.American Botanical Council, Austin, Texas, USA. First Edition.

Borowski, E. and S. Michalek. 2014. 'The Effect of Chilling Temperature on Germination and Early Growth of Domestic and Canadian Soybean (Glycine max (L) Merr.) Cultivars'. Acta Scientiarum Polonorum, Hortorum Cultus,13(2): 31-43.

Chebouti-Meziou, N., A. Merabet, Y. Chebouti, F.Z. Bissaad, N. Behidj-Benyounes and S. Doumandji. 2014. 'Effect of cold and scarification on seeds germination of Pistacia atlantica L. for rapid multiplication'. Pakistan Journal of Botany, 46 (2): 441-446.

Chetouani, M., I. Mzabri, A. Aamar, A. Boukroute, N. Kouddane and A. Berrichi. 2017. 'Effect of gibberellic acid (AG₃) on the germination of seeds of Thymus satureioides L and Lavandula dentata.' Journal of Materials and Environmental Sciences, 8 (3): 942-948.

Christie, W.W. 1999. 'The analysis of evening primrose oil'. Industrial Crops Products, 10 (2): 73–83.

Deng, Yu-C., Hui-M. Hua, J. Li and P. Lapinskas. 2001. 'Study the cultivation and uses of evening primrose (Oenothera spp.) in China'. Economic Botany, 55 (1): 83-92.

El-Dengawy, E. R.F. and A. A. Hussein. 2014. 'The effects of treating persimmon (Diospyros lotus) seeds with moist-chilling and growth regulators on seeds germination, the subsequent seedling characters and their induced drought tolerance'. IOSR Journal of Agriculture and Veterinary Science, 7 (9): 45-53.

Fetouh, M. I. and F. A. Hassan. 2014. 'Seed germination criteria and seedling characteristics of Magnolia grandiflora L. trees after cold stratification treatments'. International Journal of Current Microbiology and Applied Sciences, 3(3): 235-241.

Fieldsend, A. F. and J. I. Morison. 2000. 'Climatic conditions during seed growth significantly influence oil content and quality in winter and spring evening primrose crops (Oenothera spp.)'. Industrial crops and Products, 12(2): 137-147.

Fu, J. J., J. Liu, L.Y. Yan and Y. J. Miao. 2017. 'Effect of low temperature on seed germination, early seedling growth and antioxidant systems of the wild Elymus nutans Griseb'. Journal of Agricultural Science and Technology, 19 (5): 1113-1125.

Fukuda, M., Y. Yanai, Y. Nakano and T. Higashide. 2018. 'Differences in vernalisation responses in onion cultivars'. The Journal of Horticultural Science and Biotechnology, 93(3): 316-322.

Ghasemnezhad, A and B. Honermeier. 2007. Seed yield, oil content and fatty acid composition of Oenothera biennis L. affected by harvest data and harvest method. Industrial Crops Products, 25: 274-281.

Ghasemnezhad, A. 2007. 'Investigations on the effects of harvest methods and storage conditions on yield, quality and germination of evening primrose (Oenothera biennis L.) seeds'. (Doctoral dissertation, Universität sbibliothek Giessen). 88.

Ghasemnezhad, A. and B. Honermeier. 2012. 'Effects of Nitrogen and Pre-Harvest Desiccation on Seed Yield and Oil Quality of Evening Primrose (Oenothera biennis L.)'. Journal of Medicinal Plants and By-products, 1:61-65.

Ghildiyal, S. K., C. M. Sharma and V. P. Khanduri. 2009. 'Effect of pre-soaking and pre-chilling treatments on seed germination of Pinus roxburghii provenances from western Himalaya, India'. Journal of Forestry Research, 20 (4): 323-330.

Greiner, S. and K. Köhl. 2014. 'Growing evening primroses (Oenothera)'. Frontiers in plant science, 5(38): 1-12.

Hafez, R. F., Z. Shahabzadeh, B. Heidari and M. Ghadimzadeh. 2013. 'Investigation of the efficiency of direct and indirect regeneration in evening primrose (Oenothera biennis)'. Journal of Crop Science and Biotechnology, 16 (4): 291-296.

Hall, I. V., E. Steiner, P. Threadgill and R. W. Jones. 1988. 'The Biology of Canadian Weeds: 84. Oenothera biennis L.' Canadian journal of plant science, 68 (1):163-173.

Hosseini, M.N., M.H.  Naeimi and M. Oveisi. 2018. 'Study of seedling establishment and phenological stages of Scrophularia striata a medicinal plant in Iran'. Acta Scientific Agriculture, 2(3): 02-09.

Kaur, Y. and R. K. Dhull. 2017. 'Effect of vernalization on flowering and true seed production behavior of garlic (Allium sativum) under North Indian plains'. Indian Journal of Agricultural Sciences, 87 (11): 154-158.

Keshtkar, H. R., H. Azarnivand and H. Atashi. 2009. 'Effect of prechilling and GA₃ on seed germination of Ferula assa-foetida and Prangos ferulacea'. Seed Science and Technology, 37 (2): 464-468.

Kirmizi, S., G.  Güleryüz, H. Arslan and F. S. SAKAR. 2010. 'Effects of moist chilling, gibberellic acid, and scarification on seed dormancy in the rare endemic Pedicularis olympica (Scrophulariaceae)'. Turkish Journal of Botany, 34(3): 225-232.

Mihulka, S.,P. Pyšek  and J. Martínková. 2003. 'Invasiveness of Oenothera congeners in Europe related to seed characteristics'. Plant invasions: ecological threats and management solutions. Backhuys Publishers, Leiden,213-225.

Muhamad, S. and N. Al-Hawezy. 2013. 'The role of the different concentration of GA3 on Seed Germination and Seedling Growth of Loquat (Eriobotrya japonica L.).' Journal of Agriculture and Veterinary Science, 4 (5): 03-06.

Nagel, K. A., B. Kastenholz, S. Jahnke, D. Dusschoten, T. van.Aach, M. Muhlich, D. Trugn, H. Scharr, S. Terjung, A. Walter and U. Schurr. 2009. 'Temperature responses of roots: impact on growth, root system architecture and implications for phenotyping'. Functional Plant Biology, 36: 947-959.

Naikawadi, V.B., M.L. Ahire and T.D. Nikam 2012. 'Seed characterization, viability and promotion of seed germination in nervine tonic plant Evolvulus alsinoides Linn'. Asian Australasian Journal of Plant Science and Biotechnology, 6(1): 5-11.

Nasiri, M., A. A. Jafari, M. A. Alizadeh and S. R. Safavi. 2018. 'Effect of moist chilling treatment on seed germination of 7 species of Anthemis and Tanacetum preserved in medium storage condition in Natural Resources Gene Bank, Iran'. Iranian Journal of Seed Science and Technology, 7(1): 223-232.

Nasri, F., N. Ghaderi, J. Mohammadi, S. N. Mortazavi and S. M. Koshesh. 2013. 'The Effect of Gibberellic Acid and Stratification on Germination of Alstroemeria (Alstroemeria ligtu hybrid) Seed Under In Vitro and In Vivo Conditions'. Journal of Ornamental Plants,4(3): 222-228.

Nishiura, A., S. Kitagawa, M. Matsumura, Y. Kazama, T. Abe, N. Mizuno, S. Nasuda  and K. Murai. 2018. 'An early-flowering einkorn wheat mutant with deletions of PHYTOCLOCK 1/LUX ARRHYTHMO and VERNALIZATION 2 exhibits a high level of VERNALIZATION 1 expression induced by vernalization'. Journal of plant physiology, 222: 28-38.

Parvin, P., M. Khezri, I. Tavasolian and H. Hosseini. 2015. 'The effect of gibberellic acid and chilling stratification on seed germination of eastern black walnut (Juglans nigra L.)'. Journal of Nuts, 6 (1): 67-76.

Pasquini, N. M. and G. E. Defosse. 2012.' Effedts of storage conditions and pre-chilling periods on germinability of Pinus ponderosa seeds from Patagonia, Argentina'. Bosque Studio,33 (1): 99-103.

Patel, Ruby G. and U. MankadArchana. 2014. 'Effect of Gebberellins on Seed Germination of Tithonia rotundifolia Blake'. International Journal of Innovative Research in Science, Engineering and Technology, 3 (3): 10680-10684.

Pipinis, E., A. Stampoulidis, E. Milios, K. Kitikidou and K. Radoglou. 2017. 'Effects of cold stratification and GA3 on germination of Arbutus undo seeds of three provenances'. African Journal of Traditional, Complementary and Alternative Medicines, 14 (1): 318-323.

Razmi, Z., R. Hamidi and H. Pirasteh-Anosheh. 2013. 'Seed germination and seedling growth of three sorghum (Sorghum bicolor L.) genotypes as affected by low temperatures'. International Journal of Farming and Allied Sciences, 2 (20): 851-856.

Ren, Y. Q. and K. Y. Guan. 2008. 'Effects of moist-chilling and GA3 applications on seed germination of three Pedicularis species from Yunnan, China'. Seed Science and Technology, 36 (1): 225-229.

Rodrigues, R., I. C. Costa, F. B. Almeida, R. A. S. Cruz, A. M. Ferreira, J. C. T. Vilhena, A. C. Florentino, J. C. T. Carvalho and C. P. Fernandes, C. P. 2015. 'Development and characterization of evening primrose (Oenothera biennis L.) oil Nano emulsions'. Brazilian Journal of Pharmacognosy, 25: 422-425.

Rouhi, V., A. Ranjbarfardooei and P. Van Damme. 2003. 'Effects of gibberellic acid and temperature on germination of Amygdalus scoparia Spech. seeds'. Options Méditerranéennes, Série A, 63: 397-401.

Sarić-Krsmanović, M., J. Gajić-Umiljendić, L. Šantrić and L. Radivojević. 2015. 'Impact of storage conditions on seed germination and seedling growth of wild oat (Avena fatua L.) at different temperatures'. Pesticidi i fitomedicina, 30 (4): 243-248.

Sayyad-Amin, P. and A. R. Shahsavar. 2018. 'Improvement of Seed Germination of Date-plum (Diospyros lotus L.) by Physical and Chemical Treatments'. Journal of Chemical Health Risks, 8(4): 329-334.

Scott, S. J., R. A. Jones and W. A. Willinams. 1984. 'Analysis methods for seed germination'. Crop Science, 24 (6): 1192-1199.

Sekeroglu, N. and M. Ozguven. 2006. 'Effects of different nitrogen doses and row spacing applications on yield and quality of Oenothera biennis L. grown in irrigated lowland and unirrigated dry land conditions'. Turkish journal of agriculture and forestry, 30 (2): 125-135.

Shaban, M. 2013. 'Effect of water and temperature on seed germination and emergence as a see hydrothermal time model'. International journal of Advanced Biological and Biomedical Research,1 (12): 1686-1691.

Sincik, M., U. Bilgili, A. Uzun and Acikgoz, E. 2004. 'Effect of low temperature on the germination of different field pea genotypes'. Seed Science & Technology, 32: 331-339.

Sohrabi, O., A. Ghasemnezhad and M. S. Ahmad Nadimi. 2017. 'Seed oil quality of GA₃ induced flowering evening primrose (Oenothera biennis L.)'. Iranian Journal of Plant Physiology, 7 (2): 1971-1981.

Stejskalova, J., I. Kupka and S. Miltner. 2015. 'Effect of gibberellic acid on germination capacity and emergence rate of Sycamore maple (Acer pseudoplatanus L.) seeds'. Journal of Forest Science, 61 (8): 325-331.

Timoszuk, M., K. Bielawska and E. Skrzydlewska. 2018. 'Evening Primrose (Oenothera biennis) Biological Activity Dependent on Chemical Composition'. Antioxidants Journal, 7 (8):108-119.

Vera, D.T., R. P. Martín and S. R. Oliva. 2010. 'Effect of chemical and physical treatments on seed germination of Erica australis'. Annales Botanici Fennici-BioOne, 47: 353-361.

Vieira, A. R., M. G. G. C. Vieria, A. C.  Fraga, J. A. Oliveira and C. D. D. Santos. 2002. 'Action of Gibberellic Acid (GA3) on Dormancy and Activity of a Amylase in Rice Seeds'. Revista Brasileira de Semntes, 24 (2): 43-48.

Zadeh, S.Y., A. A. Ramin and B. Baninasab. 2015. 'Effect of gibberellic acid, stratification and salinity on seed germination of Echinacea purpurea cv. Magnus'. Herba Polonica Journal, 61(3): 13-22.