Egg Yolk Cholesterol of Hens Fed Barley Malt Rootlets

Subject Areas : Camel

S.M. Hashish ¹ , L.D. Abd El-Samee ²

1 - Department of Animal Production, National Research Centre, Dokki, 12622, Cairo, Egypt
2 - Department of Animal Production, National Research Centre, Dokki, 12622, Cairo, Egypt

Received: 2011-01-03 Accepted : 2011-05-25 Published : 2012-03-01

Keywords: laying hen, Cholesterol, lipids, Plasma, barley malt rootlets, Egg yolk,

Abstract :

This study aimed to decrease the cholesterol content of egg yolk of laying hens through inclusion of barley malt rootlets in the diets. Forty-two, 54-week-old, Lohman laying hens were fed for a 12 weeks laying period on 3 iso-caloric and iso-nitrogenous diets contained 0 (control), 25.5 or 51.1g barley malt rootlets/kg. Inclusion of barley malt rootlets at 25.5 or 51.1g/kg diet significantly decreased (P<0.001) concentrations of plasma cholesterol (by 54.9 and 33.3%, respectively) and triglycerides (by 26.4% and 27.3, respectively) compared with the control. Feeding barley malt rootlets at 25.5 g/kg diet tended to decrease (P>0.05) concentrations of total lipids, triglycerides, cholesterol, low-density lipoproteins and phospholipids in egg yolk by 4.7, 5.7, 5.2, 3.9 and 5.3% respectively, while its inclusion at 51.1 g/kg diet significantly (P<0.05) decreased the same respective parameters by 10.4, 10.4, 10.4, 10.6 and 11.22% respectively, compared with the control. It could be concluded that inclusion of barley malt rootlets in laying hen diets at 25.5 g/kg diet tended to decrease cholesterol and low-density lipoproteins in egg yolk (by 5.2 and 3.9%, respectively, while its inclusion at 51.1 g/kg diet decreased cholesterol and triglycerides concentrations in blood plasma and also decreased cholesterol and low-density lipoproteins in egg yolk (by 10.6 and 10.4%, respectively).

References:

AOAC. (1996). Official Methods of Analysis. 16^th Ed. Association of Official Analytical Chemist. Washington, D.C., U.S.A.
Ayerza R. and Coates W.(2000). Dietary levels of chia:Influence on yolk cholesterol, lipid content and fatty acid composition for two strains of hens. Poult Sci. 79,724-739.
Bonnely Y.S., Peyrat-Maillard M.N., Rondini L., Masy D. and Berset C. (2000). Antioxidant activity of malt rootlets extracts. J. Agric. Food Chem. 48, 2785-2792.
Burger W.C., Qureshi A.A., Din Z.Z., Abuirmeileh N. and Elson C.E. (1984). Suppression of cholesterol biosynthesis by constituents of barleykernels. Atherosclerosis. 51, 75-87.
Butarbutar T.B. (2004). Fatty acid and cholesterol in eggs: A review. Southeast Asian. J. Trop. Medicine Public Health. 35, 1036-1038.
Cavallero A., Alberto G., Franca F., Giovanni D. and Antonio M.S. (2004). Tocols in hull-less and hulled barley genotypes grown in contrasting environments. J. Cereal. Sci. 39, 175-180.
Chapman M.J. (1980). Animal lipoproteins: Chemistry, structure, and comparative aspects.J. Lipid Res. 21, 789-853.
Dandey D. and Bobraszczyk B.J. (2001). Cereals and Cereal Products Chemistry and Technology. Aspen Publication, Maryland.
Duncan, D.B. (1955). Multiple range and multiple F-test. Biometrics. 11, 1-42.
Eckel W. (1977). A fully enzymatic colorimetric method for determination of HDL -cholesterol in the serum. Arztl-lab. 23-101.
El-Husseiny O.M., Hanafy M.A., Radwan M.A.H. and Azouz H.M.M. (1997). Evaluation of traditional and untraditional protein sources in rabbit diets. Egyptian. J. Anim. Prod. 34, 57-66.
Folch O.J., Lees M. and Sloan-Stanely G.H. (1957). A simple method for isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226, 497-509.
Gallaher D.D., Hassel C.A., Lee K.J. and Gallaher C.M. (1993). Viscosity and fermentability as attributes of dietary fiber responsible for the hypocholesterolemic effect in hamsters. J. Nutr. 123, 240-252.
HargisP.S.(1988). Modifying egg yolk cholesterol in the domestics fowl-A review. J. World's Poult. Sci. 44, 17-29.
Jonker D., Hasselwander O., Tervilä-Wilo A. and Tenning P.P. (2010). 28-Day oral toxicity study in rats with high puritybarleybeta-glucan (Glucagel™). Food and Chemical Toxicol. 48, 422-428.
Kates M. (1972). Techniques of Lipidology: Isolation Analysis and Identification of Lipids. Amsterdam: North Holland Publishing Co.
Kaur C., Raheja R., Singh A. and Bhatia I.S. (1973). New colourimetric method for the quantitative estimation of phospholipids without acid digestion. J. Lipid Res. 41, 50-56.
Lopez H.W., Levrat M.A., Guy C., Messanger A., Demigne C. and Remesy C. (1999). Effects of soluble corn bran arabinoxylans on cecal digestion, lipid metabolism, and mineral balance (Ca, Mg) in rats.J. Nutr. Biochem. 10, 500-509.
LowellP., Foester A. and Galph T.D. (1973). Determination of triglyceride in serum. Clin. Chem. 19, 338-340.
McDonald M.W. and Shafey T.M. (1989). Nutrition of the hen and egg cholesterol. Pp. 33-39 in: Eggs Seminar. Egg Industries Research Council, Sydney, Australia.
McDonald P., Edwards R.A. and Greenhalgh J.F.D. (1995). Polysaccharides Barley by-products. Animal Nutrition. 5^th Ed. England. Pp. 22-24 and 494-499.
NRC. (1994). Nutrient Requirements of Poultry. 9^th  Ed. National Research Council, National Academy Press, Washington, DC., USA.
Qureshi A.A., Chaudhary V., Weber F.E., Chicoye E. and Qureshi N. (1991a). Effects of brewer’s grain and other cereals on lipid metabolism in chickens. Nutr. Res. 11, 159-162.
Qureshi A.A., Burger W.C., Peterson D.M. and Elson C.E. (1986). The structure of an inhibitor of cholesterol biosynthesis isolated from barley. J. Biol. Chem. 261, 10544-10552.
Qureshi A.A., Peterson D.M., Elson C.E., Mangels A.R. and Din Z.Z. (1989). Simulation of avian cholesterol metabolism by α-tocopherol. Nutrition Reports International. 40, 993-1001.
Qureshi A.A., Prentice N., Din Z.Z., Burger W.C., Elson C.E. and Sunde M.L. (1984). Influence of culture filtrate of Trichodermia viride and barley on lipid metabolism of laying hens. Lipids. 19, 250-257.
Peterson D. (1994). Barley tocols: Effects of milling, malting and mashing. Cereal Chem. 71, 42-44.
Razdan A. and Pettersson D. (1994). Effect of chitin and chitosan on nutrient digestability and plasma lipid concentration in broiler chickens. British. J. Nutr. 72, 277-288.
Razdan A., Pettersson D. and Pettersson J. (1997). Broiler chickens body weights, feed intake, plasma lipid and small-intestine bile acid concentrations in response to feeding of chitosan and pectin. British. J. Nutr.78, 283-291.
Sahin K., Sahin N. and Yaralioglu S. (2002). Effects of vitamin C and vitamin E on lipid peroxidation, blood serum metabolites, and mineral concentrations of laying hens. Biol. Trace Elem. Res. 85, 35-45.
SAS. (1996). Sas User's Guid for Personal Computers. SAS Institute Inc. Cary, Nc.USA.
Scheletter G. and Nussel E. (1975). Quantitative enzymatic Colorimetric determination of triglycerides in serum or plasma. Arbeitsmed Sozialmed Pracentimed. 10, 25.
Sharma P. and Hardeep S.G. (2010). Milling behavior of hulled barley and its thermal and pasting properties. J. Food Engineering. 97, 329-334.
Shen Ch S.J., Chen I.S. and Sheppard A.J. (1982). Enzymatic determination of cholesterol in egg yolk. J. Assoc. Off. Anal. Chem. 65, 1222-1224.
Stein E.A. (1986). Quantitative enzymatic colorimetric determination of total cholesterol in serum or plasma. In: Textbook of Clinical Chemistry. Nwtietz, ed.W. B. Saunders, Philadelphia. Pp. 879-886.
Topping D.L. (1991). Soluble fibers: effect on plasma cholesterol and colonic fermentation. Nutr. Rev. 49, 195-203.
Truswell A.S. (1999). Cereal grains and coronary heart disease. A review of the literature commissioned by "Go Grains" (gograins.com.au).
Wang L., Newman R.K., Newman C.W. and Hofer P.J. (1992). Barley β -glucans alter intestinal viscosity and reduce plasma cholesterol concentrations in chicks. J. Nutr. 122, 2292-2297.
Wang L., Newman R.K., Newman C.W., JacksonL.L. and Hofer P.J. (1993). Tocotrienol and fatty acid composition of barley oil and their effectson lipid metabolism. Plant Foods Human Nutr. 43, 9-17.
Wang L., Stephen R.B., Rosemary K., Newman R.D. and Walter C. (1997). Comparative cholesterolloweringeffects of barley β-glucan and barley oil in golden Syrian hamsters. Nutr. Res. 17, 77-88.
Weber C.W., Gentry H.S., Kohlepp E.A. and McCronan P.R. (1991). The nutritional and chemical evaluation of chia seeds. Ecol. Food Nutr. 26, 119-125.
Wieland H. and Seidel D. (1983). A fully enzymatic colourimetric determination of LDL- cholesterol in serum. J. Lipid Res. 42, 904-907.
Zeidler G. (2002). Shell eggs and their nutritional value. Pp. 1109-1128. In: Commercial Chicken Meat and Egg Production. D.D. Bell. and W.D. Weaver, Jr., ed. Kluwer Academic Publisher, New York.