Utilization of a 17 Microsatellites Set For Bovine Traceability in Czech Cattle Populations

Subject Areas : Camel

L.  Putnova ¹ (Mendel University, Zemědělská 1, 613 00 Brno, Czech, Republic Libor Stehlík)
I.  Vrtkova ² (Mendel University, Zemědělská 1, 613 00 Brno, Czech, Republic Libor Stehlík)
P.  Srubarova ³ (Mendel University, Zemědělská 1, 613 00 Brno, Czech, Republic Libor Stehlík)
L.  Stehlik ⁴ (Mendel University, Zemědělská 1, 613 00 Brno, Czech, Republic Libor Stehlík)

Received: 2010-10-26 Accepted : 2010-12-10 Published : 2011-03-01

Keywords: cattle, microsatellites, Multiplex PCR, parentage control,

Abstract :

For identification of individuals and parentage control performed by cattle breeders in the Czech Republic, a novel Finnish Bovine Genotypes™ Panel 3.1was amplified by means of one multiplex polymerase chain reaction. Bovine Panel encompasses all the 12 STR loci recommended by the International Society for Animal Genetics (ISAG) for routine use in parentage testing and identification, including TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA23, ETH3, ETH225, BM1824 and BM1818. In addition, the kit included the following six microsatellites which were among the list of loci recommended by the Food and Agriculture Organization of the United Nations (FAO) for genetic studies of domestic animals: SPS113, RM067, CSRM60, MGTG4B, CSSM66 and ILSTS006. Afterwards, the length of these microsatellite’s polymorphisms was examined by fragment analysis of the amplified products. The investigated population consisted of 125 animals of three bovine breeds (Fleckvieh, n=50; Charolais, n=50 and Beef Simmental, n=25). A total of 337 alleles were identified and all microsatellite DNA markers showed high polymorphism. The probabilities of paternity exclusion/one parental genotype unavailable/and parentage exclusion were 0.9942/0.9798/0.9999 (Fleckvieh), 0.9834/0.9744/0.9999 (Charolais), 0.9828/0.9682/0.9999 (Beef Simmental). Research data certified the possibility of using the Finnish panel of DNA microsatellites markers for the traceability purposes in the Czech cattle populations.

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