Utilization of a 17 Microsatellites Set For Bovine Traceability in Czech Cattle Populations

Subject Areas : Camel

L. Putnova ¹ , I. Vrtkova ² , P. Srubarova ³ , L. Stehlik ⁴

1 - Mendel University, Zemědělská 1, 613 00 Brno, Czech, Republic Libor Stehlík
2 - Mendel University, Zemědělská 1, 613 00 Brno, Czech, Republic Libor Stehlík
3 - Mendel University, Zemědělská 1, 613 00 Brno, Czech, Republic Libor Stehlík
4 - Mendel University, Zemědělská 1, 613 00 Brno, Czech, Republic Libor Stehlík

Received: 2010-10-26 Accepted : 2010-12-10 Published : 2011-03-01

Keywords: cattle, microsatellites, Multiplex PCR, parentage control,

Abstract :

For identification of individuals and parentage control performed by cattle breeders in the Czech Republic, a novel Finnish Bovine Genotypes™ Panel 3.1was amplified by means of one multiplex polymerase chain reaction. Bovine Panel encompasses all the 12 STR loci recommended by the International Society for Animal Genetics (ISAG) for routine use in parentage testing and identification, including TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA23, ETH3, ETH225, BM1824 and BM1818. In addition, the kit included the following six microsatellites which were among the list of loci recommended by the Food and Agriculture Organization of the United Nations (FAO) for genetic studies of domestic animals: SPS113, RM067, CSRM60, MGTG4B, CSSM66 and ILSTS006. Afterwards, the length of these microsatellite’s polymorphisms was examined by fragment analysis of the amplified products. The investigated population consisted of 125 animals of three bovine breeds (Fleckvieh, n=50; Charolais, n=50 and Beef Simmental, n=25). A total of 337 alleles were identified and all microsatellite DNA markers showed high polymorphism. The probabilities of paternity exclusion/one parental genotype unavailable/and parentage exclusion were 0.9942/0.9798/0.9999 (Fleckvieh), 0.9834/0.9744/0.9999 (Charolais), 0.9828/0.9682/0.9999 (Beef Simmental). Research data certified the possibility of using the Finnish panel of DNA microsatellites markers for the traceability purposes in the Czech cattle populations.

References:

Budowle B., Garofano P., Hellman A., Ketchum M., Sreetharan K., Parsons W., Van Haeringen W., Fain S. and Broad T. (2005). Recommendations for Animal DNA Forensic Identity Testing. Int. J. Legal Med. 119, 295-302.
Cervini M., of Brazilian Nellore cattle (Bos indicus). Genet Henrique-Silva F., Mortari N. and Matheucci Jr M. (2006). Genetic variability of 10 microsatellite markers in the characterization. Mol. Biol. 29(3), 486-490.
Choroszy B., Janik A., Choroszy Z. and Zabek T. (2006). Polymorphism of selected microsatellite DNA sequences in Simmental cattle chosen for identification of QTLs for meat traits. Animal Science Papers and Reports. 24(Suppl 2), 71-77.
Civáňová K. and Putnová L. (2003). Informativeness evaluation of the microsatellite markers used for parentage testing in cattle. In: Sb. Proceedings of Ph.D. students conference MendelNet´03, 25.11.2003. 53-59, ISBN 80-7157-723-5.
Civáňová K., Dvořák J. and Putnová L.(2003). Frekvence mikrosatelitních alel u masných plemen skotu v ČR. In: Sb. Current Problems of Breeding, Health, Growth and Production of Cattle. České Budějovice 19.-20.2.2003. 49. ISBN 80-85645-47-5.
Czerneková V., Kott T., Dudková Z., Sztankóová A. and Soldát J. (2006). Genetic diversity between seven Central European cattle breeds as revealed by microsatellite analysis. Czech J. Anim. Sci. 51, 1-7.
Glowatzki-Mullis M.L., Muntwyler J. and Gaillard C. (2006).Cost-effective parentage verification with 17plex PCR for goat and 19plex PCR for Wheel. In: Proceedings of the 30^th International Conference on Animal Genetics, 2006, Porto Seguro, Brazil. Belo Horizonte, Brazil: CBRA, 2006. A088: 23. ISBN 85-85584-02-5.
Jamieson A. and Taylor St C.S. (1997). Comparisons of three probability formulae for parentage exclusion. Anim. Genet. 28, 397-400.
Koskinen M.T. (2006). Development of STR assays for identification and forensic testing.In: Proceedings of the 30^th International Conference on Animal Genetics, 2006, Porto Seguro, Brazil. Belo Horizonte, Brazil: CBRA, 2006. W582: 21.
MangaI., Putnova L., Riha J., Vrtkova I. and Dvorak J. (2007). Genetic characterization of the Czech Spotted cattle breed using panel of 10 microsatellite markers. In Sb. Book of Abstracts of the 58^th Annual Meeting of the European Association for Animal Production, Dublin, Ireland, August 26^th-29^th 2007. 95. ISBN 978-90-8686-045-6, ISSN 1382-6077.
Maudet C., Luikart G. and Taberlet P. (2002). Genetic diversity and assignment tests among seven French cattle breeds based on microsatellite DNA analysis. J. Anim. Sci. 80(4), 942-950.
MontoyaA.E., Cerón-MuñozM.F., Moreno M.A., MartínezE., Juan D., CorralesJ.D., TiradoJ.F., Samir J. and CalvoS.J. (2010). Genetic characterization of the Hartón del Valle, Angus, Brangus, Holstein, and Senepol cattle breeds in Colombia, using ten microsatellite markers. Revista Colombiana de Ciencias Pecuarias. 23(3), 283-291.
Radko A., Zzyga A., Zabek T. andSlota E. (2005). Genetic variability among Polish Red, Hereford and Holstein-Friesian cattle raised in Poland based on analysis of microsatellite DNA sequences. J. Appl. Genet. 46(1), 89-91.
Radko A. (2010). Application of a complementary set of 10 microsatellite DNA markers for parentage verification in Polish Red Cattle. Annal. Anim. Sci. 10(1), 9-15.
Řehout V., Hradecká E. and Čítek J. (2006). Evaluation of parentage testing in the Czech population of Holsteincattle. Czech J. Anim. Sci. 51(12), 503-509.

Stevanovic J., Stanimirovic Z., Dimitrijevic V., Stojic V., Fratric N. and Lazarevic M. (2009). Microsatellite DNA polymorphism and its usefulness for pedigree verification in Simmental cattle from Serbia. Acta Veterinaria (Beograd). 59(5-6), 621-631.

Stevanovic J., Stanimirovic Z., Dimitrijevic V. and Maletic M. (2010). Evaluation of 11 microsatellite loci for their use in paternity testing in Yugoslav Pied cattle (YU Simmental cattle). Czech J. Anim. Sci. 55(6), 221-226.

Van de Goor L.H., Koskinen M.T. andVan Haeringen W.A. (2009). Population studies of 16 bovine STR loci for forensic purposes. Int. J. Legal Med.(Epub ahead of print, doi: 10.1007/s00414-009-0353-8).