Effect of foliar application of urea and salicylic acid on morphological traits and phytochemical compounds of Physalis alkekengi L.

Subject Areas : Medicinal Plants

Ali Pezeshki ¹ , Hassan Nourafcan ² , Mehdi Oraei ³ , Naser Mohebalipour ⁴ , Asad Asadi ⁵

1 - Department of Horticultural Science, Faculty of Agriculture, Miyaneh Branch, Islamic Azad University, Miyaneh, Iran,
2 - Department of Horticultural Science, Faculty of Agriculture, Miyaneh Branch, Islamic Azad University, Miyaneh, Iran,
3 - Department of Horticultural Science, Faculty of Agriculture, Miyaneh Branch, Islamic Azad University, Miyaneh, Iran,
4 - Department of Agronomy and Plant Breeding, Faculty of Agriculture
5 - Department of Veterinary Medicine, Miyaneh Branch, Islamic Azad University, Miyaneh, Iran

Received: 2021-11-07 Accepted : 2022-01-01 Published : 2022-06-03

Keywords: Salicylic acid, Foliar application, Urea, Phytochemical, physalis alkekengi L,

Abstract :

Physalis alkekengi L. belongs to the Solanacae family and its fruit is widely used in traditional medicine to treat diseases such as kidney and urinary tract stones, gout and hepatitis. Since the use of biostimulants in agriculture can be effective in improving the quantity and quality of active ingredients of medicinal plants and improving their yield, in this study, to evaluate the effect of salicylic acid and urea foliar application on morphological characteristics and phytochemical composition of this plant an experiment in a completely randomized design in research greenhouse of Islamic Azad University, Miyaneh Branch in 2019 was conducted. Spraying salicylic acid (0.001, 0.0025, 0.005 and 0.01 mM) and urea (0.25, 0.5, 1 and 2%) was applied in three stages with an interval of 10 days in three repetitions. Extraction was performed simultaneously with harvest (mid-September) by soaking method and the amount of phytochemical compounds in the shoot was determined using HPLC. Based on the results of variance analysis, the effect of foliar application on all morphological traits of shoots (except leaf width), root morphological traits and yield traits was significant. Increasing the concentration of urea and salicylic acid in most of the measured traits showed a positive effect. The highest increase in the mentioned traits was mainly related to the concentrations of 1% and 2% urea and salicylic acid 0.01 mM. Compared to the control, concentrations of 1% urea and 0.01 mM salicylic acid increased the number of fruits (76.8% and 64.6%, respectively), fruit diameter (17.3% and 25.7%, respectively), fruit length (15.07 and 33.4%, respectively), fresh weight of shoots (29.9 and 56.4%, respectively) and dry weight of shoots (28.4 and 61.4%, respectively). In addition, with increasing salicylic acid concentration the amount of luteolin, quercetin, campferol, ferulic acid and chlorogenic acid increased compared to the control treatment.

References:

1. Abbaszadeh, B., Sharifi Ashourabadi, E., Ardakani, M.R. and Paknejad, F. 2005. Effect of spraying of nitrogen fertilizer on Melissa officinalis yield in the greenhouse condition. Iranian Journal of Medicinal and Aromatic Plants, 21(2): 207-216. (In Persian).


2. Abbaszadeh, B., Sharifi Ashourabadi, E., Ardakani, M.R., Lebaschi, M.H., Safikhani, F. and Naderi Hadjibagher Kandi, M. 2006. Effect of application methods of nitrogen fertilizer on essential oil content and composition of balm (Melissa officinalis) under field condition. Iranian Journal of Medicinal and Aromatic Plants, 22(3): 223-230. (In Persian).


3. Abdel, F. and Gharib, L. 2006. Effect of salicylic acid on the growth, metabolic activities and oil content of basil and marjoram. International. Journal of Agricultural Biology, 4: 458-492.


4. Akinsulier, R.O., Aibinu, I.E., Adenipekun, T., Adelowotan, T. and Odugbemi, T. 2007. Invitro antimicrobial activity of crude extracts from plants Bryophyllum pinnatum and Kalanchoe crenata. African Journal Traditional, 4(3): 334-348.


5. Amiri, A., Yadolahi, P., Siroosmehr, A. and Esmaeilzadeh, S. 2015. Effect of drought stress and chitosan and salicylic spray on morphological parameters of Carthamus tinctorius in Sistan. Journal of Oil Plant Production, 2: 1. 43-56. (In Persian)


6. Bayat, H., Alirezaie, M. and Neamati, H. 2012. Impact of exogenous salicylic acid on growth and ornamental characteristics of Calendula (Calendula officinalis) under salinity stress. Journal of Stress Physiology and Biochemistry, 8: 258-267. (In Persian).


7. Beaudoin-Eagan, L.D. and Thorpe, T.A. 1985. Tyrosine and phenylalanine ammonia lyase activities during shoot initiation in tobacco callus cultures. Plant Physiology, 78: 438-441.


8. Chen, J.Y., Wen, P.F., Kong, W.F., Qiu-Hong, P., Ji-Cheng, Z., Jing-Ming, L., Si-Bao, W. and Wei-Dong, H. 2006. Effect of salicylic acid on phenylpropanoids and phenylalanine ammonia-lyase in harvested grape berries. Postharvest Biology and Technology, 40: 64-72.


9. Darvizheh, H., Zahedi, M. and Abbaszadeh, B. 2019. Effects of foliar application of salicylic acid and spermine on the growth and root morphological characteristics of purple coneflower (Echinacea purpureal) under drought stress. Journal of Plant Process and Function, 8(30): 225-242. (In Persian).


10. Eraslan, F., Inal, A., Gunes, A. and Alpaslan, M. 2007. Impact of exogenous salicylic acid on growth antioxidant activity and physiology of carrot plants subjected to combined salinity and boron toxicity. Scientia Horticulturae, 113:120-128.


11. Eskandari Zanjani, K., Shirani Rad, A.H., Moradi Agdam, A. and Taherkhani, T. 2013. Effect of salicylic acid application under salinity conditions on physiologic and morphologic characteristics of artemisia (Artemisia annua). Journal of crop Ecophysiology, 6(4): 415-428. (In Persian).


12. Forouzandeh, M., Mohkami, Z. and Fazelinasab, B. 2019. Evaluation of biotic elicitors foliar application on functional changes, physiological and biochemical parameters of fennel (Foeniculum vulgare). Journal of Plant Production Research, 25: 49-65. (In Persian).


13. Ghasemi Pirbalouti, A., Nekoei, M., Rahimmalek, M. and Malekpoor, F. 2019. Chemical composition and yield of essential oil from lemon balm (Melissa officinalis) under foliar applications of jasmonic and salicylic acids. Biocatalysis and Agricultural Biotechnology, 19: 101-114.


14. Guleria, S., Sohal, B. and Mann, A. 2005. Salicylic acid treatment and/or Erysiphe polygoni inoculation on phenylalanine ammonia-lyase and peroxidase content and accumulation of phenolics in pea leaves. Journal of Vegetable Science, 11: 71-79.


15. Habba, E., EL-Abdel Aziz, N.G., Sarhan, A.M.Z., Arafa, A.M.S. and Youssef, N.M. 2016. Effect of putrescine and growing media on vegetative growth and chemical constituents of Populus euramericana Journal of Innovations in pharmaceuticals and Biological Sciences, 3: 61-73.


16. Hakimi, L., Naiebzadeh, M. and Khaligi, A. 2019. Investigating the effect of glycine betaine and humi-forthi on morpho-physiological and biochemical properties Pelargonium graveolens under water stress. Journal of Plant Production Research, 26(3): 37-56. (In Persian).


17. Hayati, P., Sharifi-e Shayghan, M., Hayati, P. and Rowshan Sarvestani, V. 2011. Effect of salicylic acid on morphological and physiological characteristics of sweetheart. Proceedings of the 7^th Iranian Horticultural Science Congress. Isfahan, Iran. (In Persian).


18. Ibrahim, M.H., Jaafar, H.Z., Rahmat, A. and Rahman, Z.A. 2010. The relationship between phenolics and flavonoids production with total nonstructural carbohydrate and photosynthetic rate in Labisia pumila under high CO₂ and nitrogen fertilization. Molecules, 16(1): 162-174.


19. Jamali, B., Eshghi, S. and Tafazoli, E. 2013. Vegetative growth, yield, fruit quality and fruit and leaf composition of strawberry cv. ‘Pajaro’ as influenced by salicylic acid and nickel sprays. Journal of Plant Nutrition, 36: 1043-1055. (In Persian).


20. Jamshidi, E., GHalavand, A., Sefidkon, F. and Mohammadi Goltapeh, E. 2012. Effect of different nutrition systems (organic and chemical) on quantative and qualative characteristics of Fennel (Foeniculum Vulgare) under water deficit stress. Bimontly Iranian Jouranal of Medicinal and Aromatic Plants, 28(2): 309-323. (In Persian).


21. Kahlel, A.M.S. and Sultan, F.I. 2019. Response of four potato cultivars to soil application with organic and amino acid compounds. Research on Crops, 20(1): 101-108.


22. Kashefi, B., Ghods, M. and Moghaddam, M. 2015. Salicylic acid application on some morphological and physiological traits in clary sage under salinity stress. Journal of Crops Improvement, 17(2): 431-440.


23. Khan, M.I., Fatma, M., Per, T.S., Anjum, N.A. and Khan, N.A. 2015. Salicylic acid-induced abiotic stress tolerance and underlying mechanisms in plants. Frontiers in Plant Science, 6: 462.


24. Kheiry, A., Parsa, H., Sani Khani, M. and Razavi, F. 2018. Effect of bio-fertilizers and nitrogen on quantitative and qualitative characteristics of petal in Saffron (Crocus sativus). Saffron Agronomy and Technology, 6(3):309-322. (In Persian).


25. Khodary, S.E.A. 2004. Effect of Salicylic acid on the growth, photosynthesis and carbohydrate metabolism in salt stressed maize plants. International Journal of Agriculture and Biology, 3(12): 1434-1039.


26. Khoshpeyk, S., Sadrabadi Haghighi, R. and Ahmadian, A. 2017. The effect of application of nitrogen fertilizer and nano-organic manure on yield, yield components and essential oil of Fennel (Foeniculum vulgar). Iranian Journal of Field Crops Research, 14(4):775-787. (In Persian).


27. Kovacik, J., Backo, M. and Kadukova, J. 2008. Physiological responses of Matricaria chamomilla to cadmium and copper excess. Environmental Toxicology, 23: 123-130.


28. Kumar, D., Mishra, D.S., Chakraborty, B. and Kumar, P. 2013. Pericarp browning and quality management of litchi fruit by antioxidants and salicylic acid during ambient storage. Journal of Food Science and Technology, 50: 797-802.


29. Mardaninejad, S., Kholdbarin, B., Saadat, Y. and Morad Shahi, A.S. 2003. Vegetative behaviors and the amount of lavender essential oil in response to different amounts of ammonium nitrate. Journal of Research in Iranian Medicinal Plants and Herbs, 19(1): 15-35. (In Persian).


30. Mendoza, A.B., Godina, F.R., Torres, V.R., Rodriguez, H.R. and Maiti, R.K. 2002. Chilli seed treatment with salicylic and sulfosalicylic acid modifies seedling epidermal anatomy and cold stress tolerance. Crop Research, 24(1): 19-25.


31. Miura, K. and Tada, Y. 2014. Regulation of water, salinity, and cold stress responses by salicylic acid. Plant Science Journal, 5: 410.


32. Najafian, S., Negahban, M., Tarakemeh, A. and Ghasemiyan, S.M. 2009. Effect of salicylic acid on some morphological and physiological characteristics of chamomile. 6^th Iranian Horticultural science congress, Rasht, Iran, 1117-1119. (In Persian).


33. Nourafcan, H. and Mahboubi, A. 2017. The effect of salicylic acid foliar spraying on morphophysiological characteristics of common mallow and Moldavian balm. Agroecology Journal, 13(3): 25-33. (In Persian).


34. Pérez-Balibrea, S., Moreno, D.A. and García-Viguera, C. 2011. Improving the phytochemical composition of broccoli sprouts by elicitation. Food Chemistry, 129: 35-44.


35. Picard, D., Ghiloufi, M., Saulas, P. and Tourdonnet, S. 2010. Does under sowing winter wheat with a cover crop increase competition for resources and is it compatible with high yield? Field Crops Research, 115: 9–18. Razavi Publications, Beh Nashr. 438 pp. (In Persian).


36. Popova, L.P., Maslenkova, L.T., Yordanova, R.Y., Ivanova, A.P., Krantev, A.P., Szalai, G. and Janda, T. 2009. Exogenous treatment with salicylic acid attenuates cadmium toxicity in pea seedlings. Plant Physiology and Biochemistry, 47: 224-231.


37. Rabie, M.A., Soliman, A.Z., Diaconeasa, Z.S. and Constantin, B. 2015. Effect of pasteurization and shelf life on the physicochemical properties of Physalis (Physalis peruviana) juice. Journal of Food Processing and Preservation, 39 (6): 1051-1060.


38. Radwan, D.E.M. 2012. Salicylic acid induced alleviation of oxidative stress caused by clethodim in maize (Zea mays) leaves. Pesticide Biochemistry and Physiology, 102: 182-188.


39. Sabeti Amizhendeh, M.A., Fallah Nosrat Abadi, A., Norouzi, M., Amiri, A. and Azarpour, A. 2011. Effect of nitrogen and azotobacter on some quantitative and qualities characteristics garmkhane'e tobacco (Nicotiana tabacum). Journal of Soil and Water, 22(2): 149-135. (In Persian).


40. Saharkhiz, M.J. and Goudarzi, T. 2014. Foliar application of salicylic acid changes essential oil content and chemical compositions of peppermint (Mentha piperita). Journal of Essential Oil Bearing Plants, 17: 435-440.


41. Shakirova, M.F., Sahabutdinova, A.R., Bezrukova, M.V., Fatkhutdinova, R.A. and Fatkhutdinova, D.R. 2003. Changes in the hormonal status of wheat seedlings induced by salicylic acid and salinity. Plant Science, 164.3: 317-322.


42. Travlos, I.S. 2012. Invasiveness of cut-leaf ground-cherry (Physalis angulate) populations and impact of soil water and nutrient availability. Chilean Journal of Agricultural Research, 72: 358-363.


43. Wang, Z., Ma, L., Zhang, X., Xu, L., Cao, J. and Jiang, W. 2015. The effect of exogenous salicylic acid on antioxidant activity, bioactive compounds and antioxidant system in apricot fruit. Scientia Horticulture, 181: 113-120.


44. Yavas, I. and Unay, A. 2016. Effects of zinc and salicylic acid on wheat under drought stress. The Journal of Animal and Plant Sciences, 26(4):1012-101.