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Manuscript ID : EJMP-2108-1645 (R1) Visit : 107 Page: 59 - 72

10.30495/ejmp.2022.1936932.1645

Article Type: Original Research

Essential oil composition and effect of different treatments on seed dormancy breaking and germination of Salvia sahendica Boiss. & Buhse

Subject Areas : Medicinal Plants

ahad  hedayati 1 (Assistant Professor, Academic Center for Education, Culture and Research (ACECR), West Azarbayjan Branch, West Azerbaijan Branch, Urmia, Iran)
fatemeh  Aghamohseni 2 (M.Sc., Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran)
elnaz  norouzi 3 (Assistant Professor, Academic Center for Education, Culture and Research (ACECR), West Azarbayjan Branch, West Azerbaijan Branch, Urmia, Iran)
syavash  hemmaty 4 (M.Sc., Academic Center for Education, Culture and Research (ACECR), West Azarbayjan Branch, West Azerbaijan Branch, Urmia, Iran)
mir sajjad  mir yusefzadeh 5 (M.Sc., Academic Center for Education, Culture and Research (ACECR), West Azarbayjan Branch, West Azerbaijan Branch, Urmia, Iran)
zahra  bagheri 6 (M.Sc., Academic Center for Education, Culture and Research (ACECR), West Azarbayjan Branch, West Azerbaijan Branch, Urmia, Iran)
shabnam  mir yousefzadeh 7 (M.Sc. student, Academic Center for Education, Culture and Research (ACECR), West Azarbayjan Branch, West Azerbaijan Branch, Urmia, Iran)
mohammad hossein  Mirjalili 8 (Associate Professor, Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran)

Received: 2021-08-01 Accepted : 2022-01-16 Published : 2022-03-03

Keywords: magnetic field, germination, physiological dormancy, Essential oil, Salvia sahendica Boiss. & Buhse,

Abstract :

Salvia sahendica Boiss. & Buhse.  with antimicrobial, antioxidant and anticonvulsant properties is an endemic species of the lamiaceae family that grows in the Sahand highlands in East Azerbaijan province. The aim of the present study was to investigate the quantity and quality of essential oils and effect of different treatments on the percentage and germination rate of this species due to the low germination percentage of its seeds. The aerial parts of the plant were collected at flowering stage in the summer of 2012 from Kasai habitat in Tabriz Then, its essential oil was obtained by clevenger method and analized by GC/MS. Seed test was conducted based on a completely randomized design (CRD) with 4 replications. Treatments included gibberllin at three levels of concentration (100, 150 and 200 ppm for 24 h), three levels of wash time (12, 24 and 72 h), chilling at three levels of duration (for 24, 48 and 72 h) and magnetic treatment (50 and 130 mT for 5, 10, 20, 25 and 30 min) and control. Based on the results, the essential oil yield of this species was 0.8% by weight and the presence of 23 compounds including trans-meta-mentha-2,8-diene, spathulenol, α-Pinene, 1,8-cineol and β-pinene in the essential oil components of this plant was proved. Seed test result showed that seeds treated with 100 ppm gibberellin had the highest germination rate (47 %) and seeds treated with 130 mT for 20 min had the highest germination rate (5.83) compared to control (16) and (1/99) respectively. The results indicate that the seeds of this species have physiological dormancy and different treatments can significantly increase. 

References:


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_||_

  1. Abdani Nasiri, A., Mortazaeinezhad, F. and Taheri, R. 2018. Seed germination of medicinal sage is affected by gibberellic acid, magnetic field and laser irradiation. Electromagnetic biology and medicine. 37 (1): 50-56.
    2.
  2. Abdollahi, J., Ebrahimi, M. H., Ramshini, A., Jaafari, A., Eftekhari, A., Siah, M., Mansouri, Y. and Goharrizi, M. A. 2012. Seed germination as the major conservation issue of endemic Iranian Salvia Journal of Medicinal Plants Research. 6 (1): 37-46.
    3.
  3. Abdollahi-Ghehi, H., Sonboli, A., Ebrahimi, S.N., Esmaeili, M.A. and Mirjalili, M.H. 2019. Triterpenic acid content and cytotoxicity of some Salvia species from Iran. Natural Product Communications.14 (5): 1934578-19842722.
    4.
  4. Adhikari, B., Dhital, P.R., Ranabhat, S. and Poudel, H. 2021. Effect of seed hydro-priming durations on germination and seedling growth of bitter gourd (Momordica charantia). PloS one.16 (8):
    5.
  5. Aladjadjiyan, A. 2003. Study of the influence of magnetic field on some biological characteristic of Zea maise. Journal Central European Agriculture. 3 (2): 89–98.
    6.
  6. Cornea-Cipcigan, M., Pamfil, D., Sisea, C.R. and Mărgăoan, R. 2020. Gibberellic acid can improve seed germination and ornamental quality of selected Cyclamen species grown under short and long days. Agronomy. 10 (4):516.
    7.
  7. El Euch, S.K., Hassine, D.B., Cazaux, S., Bouzouita, N. and Bouajila, J. 2019. Salvia officinalis essential oil: Chemical analysis and evaluation of anti-enzymatic and antioxidant bioactivities. South African Journal of Botany. 120: 253-260.
    8.
  8. Fateh, E., Majnoonhosseini, N., Maddah Arefi, H. and sharif-zadeh, F. 2005. Seed dormancy methods breakage in Astragalus tribuloides', Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research. 13 (4): 345-360.
    9.
  9. Finch-Savage, W.E. and Footitt, S. 2017. Seed dormancy cycling and the regulation of dormancy mechanisms to time germination in variable field environments. Journal of experimental botany. 68 (4): 843-856.
    10.
  10. Florez, M., Martinez, E. and Carbonell M.V. 2012. Effect of magnetic field treatment on germination of medicinal plants Salvia officinalis and Calendula officinalis L. Polish Journal of Environmental Studies. 21 (1): 57-63
    11.
  11. Ghodsirasi, H., Sepehry, A. and Barani, H. 2021. Investigating changes in gibberellin and abscisic acid in the leaves and twigs of different age groups Kochia prostrata (L) schrad and its effect on germination and seed dormancy characteristics under prechilling treatment. Journal of Plant Production Research.28 (2):.23-38.
    12.
  12. Goren, A. C., Kilic, T., Dirmenci, T. and Bilsel, G. 2006. Chemotaxonomic evalution of Turkish species of Salvia: fatty acid composition of seeds oils. Biochemical Systematics and Ecology. 34 (3): 160-164.
    13.
  13. Hedayati, A., Mirjalili, M.H. and Hadian, J. 2017. Chemical diversity in the essential oil from different plant organs of Salvia sahendica & Buhse. Journal of Plant Research (Iranian Journal of Biology). 29 (4): 908-918.
    14.
  14. Humphries, T., Chauhan, B.S. and Florentine, S.K. 2018. Environmental factors effecting the germination and seedling emergence of two populations of an aggressive agricultural weed; Nassella trichotoma. PLoS One.13 (7): 199491.
    15.
  15. Lee, J.W., Jo, I.H., Kim, J.U., Hong, C.E., Kim, Y.C., Kim, D.H. and Park, Y.D. 2018. Improvement of seed dehiscence and germination in ginseng by stratification, gibberellin, and/or kinetin treatments. Horticulture, Environment, and Biotechnology.59 (3): 293-301.
    16.
  16. Liu Y., Li, Y., Luo W., and Liu, Sh. 2020. Soil potassium is correlated with root secondary metabolites and rootassociated core bacteria in licorice of different ages. Journal of Plant soil. 456: 61-79.
    17.
  17. Lotfipour, F., Samiee, M. and Nazemiyeh, H. 2007. Evaluation of the antibacterial activity of Salvia sahendica and Phlomis caucasica. Journal of Faculty of Pharmacy, Tabriz University of Medical Sciences. 1: 29-34.
    18.
  18. Mirjalili, S. 2016. Assessment of genetic diversity among some accessions of Sage (Salvia officinalis) using electrophoresis of seed storage proteins. Iranian journal of plant biology. 8 (28): 79-107.
    19.
  19. Mofidi Tabatabaei, S., Salehi, P., Moridi Farimani, M., Neuburger, M., De Mieri, M., Hamburger, M. and Nejad-Ebrahimi, S. 2017. A nor-diterpene from Salvia sahendicaNatural product research. 31 (15): 1758-1765.
    20.
  20. Nimmi, V. and Madhu, G. Effect of pre-sowing treatment with permanent magnetic field on germination and growth of Chilli (Capsicum annum. L.). International Agrophysics. 23 (5): 195-198.
    21.
  21. Norouzi, R. and Norouzi M. 2018. The study of quantitative and qualitative variations in essential oil of Salvia reuterana in wild and field conditions. Journal of Plant Process and Function.7 (23) :347-360.
    22.
  22. Nourafcan, H. and Naseri, F. 2018. Effect of bio and hormonal priming on seed germination indices of clary Sage (Salvia sclarea). Journal of Seed Research. 8 (3): 51-60.
    23.
  23. Olmez, Z., Gokturk, A. and Temel, F. 2007. Effect of cold stratification, sulfuric acid, submersion in hot and top water pretreatment on germination of bladder-senna (Colutea armena Boiss & Huet.) seeds. Seed science and technology. 35 (6): 266-271.
    24.
  24. Ozcan, M., Tzakou, O. and Couladis, M. 2003. Essential oil composition of Turkish herbal tea (Salvia aucheri) bentham var. caescens Boiss et Helder. Flavour and Fragrance Journal. 18 (5): 325-327.
    25.
  25. Paiva, E.P.D., Torres, S.B., Alves, T.R.C., Sá, F.V.D.S., Leite, M.D.S. and Dombroski, J.L.D. 2018. Germination and biochemical components of Salvia hispanica seeds at different salinity levels and temperatures. Acta Scientiarum Agronomy. 40.
    26.
  26. Paiva, E.P.D., Torres, S.B., Sá, F.V.D.S., Nogueira, N.W., Freitas, R.M.O.D. and Leite, M.D.S. 2016. Light regime and temperature on seed germination in Salvia hispanicaActa Scientiarum. Agronomy. 38: 513-519.
    27.
  27. Palizdar, M., Tavakkol Afshari, R., Jamzad, Z., Ardakani, M. and Nejat Khah, P. 2021. Evaluation of the effects of seed treatment with GA3 and pre-chilling on seed germination characteristics of Salvia species. Iranian Journal of Seed Science and Technology. 9 (4): 27-40.
    28.
  28. Rustaiyan, A., Korneilhdeh, H., Masoudi, SH. and Jassbi, A. R. 2013. Composition of the essential oil of Salvia sahendica & Buhse. Journal of Essential Oil Research. 9: 713-714.
    29.
  29. Salehi, P., Sonboli, A., Ebrahimi, S.N. and Yousefzadi, M. 2007. Antibacterial and antioxidant activities of the essential oils and various extracts of Salvia sahendica in different henological stages. Chemistry of Natural Compounds. 43 (5): 328-330.
    30.
  30. Shen, H., L. Zhu, Q.Y. and Huq E. 2012. Max2 affects multiple hormones to promote hoto morphogenesis. Molecular Plant. 5 (3): 224-236.
    31.
  31. Tuan, P.A., Kumar, R., Rehal, P.K., Toora, P.K. and Ayele, B.T. 2018. Molecular mechanisms underlying abscisic acid/gibberellin balance in the control of seed dormancy and germination in cereals. Frontiers in Plant Science. 9: 668.
    32.
  32. Ulgen, C., Yıldırım, A.B. and Turker, A.U. 2017. Effect of magnetic field
    treatments on seed germination of Melissa officinalisInternational Journal of Secondary Metabolite. 4 (3): 43-49.
    33.
  33. Vishal, B. and Kumar, P.P. 2018. Regulation of seed germination and abiotic stresses by gibberellins and abscisic acid. Frontiers in Plant Science. 9: 838.
    34.
  34. Wang, Y., Zhang, J., He, W., Yang, S. and Wang, X. 2020. Effect of gibberellin treatment on dormancy-breaking and germination of cherry seeds. In IOP Conference Series: Earth and Environmental Science. 446 (3): 032079.
    35.

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