Investigation of antioxidant activity, total phenol and flavonoid content of lemon balm (Melissa officinalis L.) in different media under hydroponic condition

Subject Areas : Medicinal Plants

elham farrokhi ¹ , abbas samadi ² , amir rahimi ³

1 - Department of Soil Science, Faculty of Agriculture, Urmia University
2 - soil science- faculty of agriculture- urmia university
3 - faculty member

Received: 2019-01-01 Accepted : 2019-05-01 Published : 2021-02-19

Keywords: Peat moss, phenol, Lemon Balm, : antioxidant, perlite size,

Abstract :

Hydroponic culture has several advantages such as higher yield, product uniformity improvement, and better control of elements absorption than soil condition. The hydroponic is a method which organic or inorganic substrates are used for plant cultivation. In order to investigate the effect of different growing media on antioxidant properties of lemon balm (Melissa officinalis), an experiment was done based on completely randomized design using 11 treatments with three replications at Experimental Greenhouse of Urmia University. Growing media tested were: perlite substrate with grads: <0.5, 0.5-1 ,1-1.5 ,1.5-2 and >2 mm as 100% and mixed grades of perlite with peat moss (50:50) and pure peat moss (100% V). The total phenolic content, total flavonoid, and antioxidant activity were measured by using folin ciocalteu reagent, aluminum chloride, and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) methods respectively. The results showed that significant differences were observed the measured parameters in inorganic (perlite with different sizes), organic (peat most), and mixture of organic and inorganic substrates. The highest content of total phenol (0.63mg GAA/100g), total flavonoid (0.07 mg Q/100g), total anthocyanin (1.7 mg/gdw), superoxide radical scavenging activity (55.3%) and radical scavenging activity DPPH (67.8%) were observed in pure peat moss medium, while the lowest nitric oxide radical scavenging activity (67%) was in peat moss medium. In conclusion different culture media which possess different physical and chemical characteristics, can affect the plant antioxidant properties. According to the results, peat moss substrate increased the antioxidant properties of lemon balm compared to other culture media

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