Phylogenetic survey of chloroplast matK sequences in some medicinal plant species of Mentheae tribe (Lamiaceae) with emphasis on Zhumeria majdae Rech. & Wendelbo.

Subject Areas : Genetic resources

Hamed Khodayari ¹ , Elham Khalili ² , fourogh Sanjarian ³ , Yunes Asri ⁴

1 - Associate Professor, Department of Biology, Faculty of Basic Science, Lorestan University, Khorramabad, Iran
2 - Ph.D. student, Department of Medical Biotechnology, Mashhad University of Medical Sciences, Mashhad, Iran
3 - Assistant Professor, Department of Plant byproducts, National Institute of Genetic Engineering and Biotechnology, Shahrak-e Pajoohesh,Tehran , Iran
4 - Associate Research, Botany Research Division, Research Institute of Forests and Rangelands, Tehran, Iran

Received: 2016-01-22 Accepted : 2016-06-22 Published : 2016-09-01

Keywords: Iran, Phylogeny, DNA Sequencing, Lamiaceae MatK gene, Zhumeria majdae Rech. & Wendelbo,

Abstract :

The aim of this research is elucidation of molecular taxonomic and phylogenetic relationship between Zhumeria and other genera of Mentheae tribe using sequencing of chloroplastic matk gene. The aim of this research is taxonomic and phylogenetic status of Mentheae tribe with emphasis Zhumeria majdae Resh. f. & Wendelbo. (Moorkhosh) belonged to Lamiaceae family that endemic of Hormozgan province, Iran and it have not been reported in elsewhere, using sequencing of chloroplastic gene matK. Zhumeria majdae has long been used in traditional medicine as antispasmodic, antimicrobial, carminative especially in infants and for dysmenorrheal. The therapeutic benefits of medicinal plants are often attributed to their antioxidant properties. It is used for treatment of the stomach ulcer, headache, reduce pain, cold and healing of wounds. The methods are as follow: Extraction of DNA, amplification of a part of matk by specific primers, DNA sequencing, and sequence analysis by FINTCH TV software, drawing phylogenetic tree by PAUP software.  The heuristic search of the Cladogram analysis resulted in 10000 shortest trees, 3 tree with lengh of 20 steps and statistic coefficient (Retention index = 0.963) and Consistencies index=0.950. In the trees resulting from maximum parsimony and maximum likelihood methods, Mentheae tribe was monophyletic and composed of three distinct monophyletic clades (subtribes Menthinae, Salviinae and Nepetinae). The The tree derived from the combined data matrix, including in-group and out-group taxa, has a total of 74 sequences. results of this study showed that Zhumeria majdae grouped with Salvia clad. Our results comfirm previous studies done by morphological, trnL-F, psbA-trnH and ribosomal DNA phylogeny.  

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