Evaluation of the antibacterial and antioxidant effects of the total extract and fractions prepared from Salvia tebesana Bunge leaves

Subject Areas : Medicinal Plants

Mohammad Rahimi ¹ , Masoud Yousefi ² , Samira Eghbali ^{3 *}

1 - Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran
2 - Department of Microbiology, Birjand University of Medical Sciences
3 - Department of Pharmacognosy, Faculty of Pharmacy, Birjand University of Medical Sciences, Iran

Received: 2025-03-10 Accepted : 2025-05-14 Published : 2025-06-16

Keywords: antioxidant, antibacterial, column chromatography, Salvia tebesana,

Abstract :

Salvia tebesana, a member of the mint family (Lamiaceae), is known for its medicinal and edible leaves. This study aims to analyze the phytochemical composition of Salvia tebesana and investigate the antioxidant and antibacterial effects of its methanol extract and fractions obtained through chromatography. The dried extract was fractionated using column chromatography, and the antioxidant and antibacterial activities were evaluated through the DPPH assay and broth microdilution method to determine the minimum inhibitory concentration (MIC). A total of 51 fractions were isolated and grouped into 8 fractions based on similar thin layer chromatography (TLC) results. The fractions exhibited varying levels of DPPH free radical scavenging activity, with fraction 5 showing the highest activity at 26.12±1.14 µg/mL and fraction 1 showing the lowest at 105.3±1.67 µg/mL. Fraction 4 had the strongest antibacterial effect against Staphylococcus aureus and Enterococcus faecalis, with an MIC of 3.125 mg/mL. The methanol extract of S. tebesana contained a wide range of phytochemical compounds responsible for its antioxidant and antibacterial properties, making it a potential candidate for the development of new agents for pathological disorders.

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