Evaluation of phytochemical compounds and antioxidant activity of white dead nettle (Lamium album L.) native to Mazandaran province

Subject Areas : Botanical

Amin Ranjbar ¹ , Alireza LadanMoghadam ^{2 *} , Shahroz Habibi Kutani ³ , Razieh Naderkhani ⁴ , Mehdi Maham ⁵

1 - Department of Horticulture, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran
2 - Department of Horticulture Sciences, Garmsar Branch, Islamic Azad University, Garmsar, Iran
3 - Department of Agriculture, Gorgan Branch, Islamic Azad University, Gorgan, Iran
4 - Department of Mathematics and Statistics, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran
5 - Department of Chemistry, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran

Received: 2025-01-04 Accepted : 2025-03-05 Published : 2025-06-16

Keywords: Antioxidant, Essential oil, Phenolic compounds, Flavonoids, Lamium album L. ,

Abstract :

 White dead nettle (Lamium album L.) is a medicinal plant with diverse properties. It has been highly regarded in traditional medicine and scientific research for its significant therapeutic and biological capabilities. This study aims to evaluate the phytochemical compounds and antioxidant activity of white dead nettle in Mazandaran province. Plant samples were collected from the vicinity of the Lega summer village in Ramsar city and dried in the shade after identification. Essential oil was extracted using water distillation with a Clevenger apparatus, and its compounds were identified and quantified using a GC/MS apparatus. The methanol extract was obtained using the Soxhlet method, and the Folin-Ciocalteau and aluminum chloride methods were used to measure phenolic and flavonoid compounds, respectively. The antioxidant activity of the extract was evaluated using the DPPH method. The most significant constituents of the white dead nettle plant were found to be p-Xylene (34.19%), Germacrene D (22.03%), o-Xylene (13.08%), and m-Xylene (10.76%). The total phenol content in the extract was 128.15 ± 0.2 mg gallic acid equivalent per gram of sample, while the flavonoid content was 28.22 ± 0.1 mg rutin equivalent per gram of sample. The DPPH free radical scavenging activity of the white dead nettle extract was observed with an IC50 of 1586.89 μg/g. Overall, the results of this study suggest that white dead nettle has high potential for application in the pharmaceutical and food industries due to its diverse properties. 

 

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