Evaluation of Phytochemical Compounds and Antioxidant Properties of White Dead Nettle (Lamium album L.) Native to Mazandaran Province

Subject Areas : Botanical

Amin Ranjbar ¹ , Alireza LadanMoghadam ^{2 *} , Shahroz Habibi Kutani ³ , Razieh Naderkhani ⁴ , Mehdi Maham ⁵

1 - Department of Horticulture, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran
2 - Department of Horticulture Sciences, Garmsar Branch, Islamic Azad University, Garmsar, Iran
3 - Department of Agriculture, Gorgan Branch, Islamic Azad University, Gorgan, Iran
4 - Department of Mathematics and Statistics, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran
5 - Department of Chemistry, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran

Received: 2025-01-04 Accepted : 2025-03-05 Published : 2025-06-16

Keywords: Antioxidant, Essential oil, Phenolic compounds, Flavonoids, Lamium album L. ,

Abstract :

White dead nettle (Lamium album L.) is a medicinal plant with diverse properties. It has been highly regarded in traditional medicine and scientific research and has significant therapeutic and biological capabilities. Accordingly, the present study evaluates the phytochemical compounds and antioxidant properties of white dead nettle in Mazandaran province. Plant samples were collected from the vicinity of the Lega summer village of Ramsar city and, after identification, were dried in the shade. The essential oil extraction was performed using water distillation using a Clevenger apparatus, and essential oil compounds were identified and quantified using a GC/MS apparatus. The methanol extract was extracted using the Soxhlet method, and phenolic and flavonoid compounds were measured using the Folin-Ciocalteau and aluminium chloride methods, respectively. Also, the antioxidant activity of the extract was evaluated using the DPPH method. The compounds p-Xylene (34.19%), Germacrene D (22.03%), o-Xylene (13.08%), and m-Xylene (10.76%) are the most important constituents of the white dead nettle plant. The amount of total phenols in the extract was 128.15 ± 0.2 mg gallic acid equivalent per gram of sample; the amount of flavonoids was 28.22 ± 0.1 mg rutin equivalent per gram of sample, and the DPPH free radical scavenging activity for the white dead nettle extract was observed with an IC50 of 1586.89 μg/g. In general, the results of this study showed that white dead nettle is of high potential for application in the pharmaceutical and food industries due to its diverse properties.

 

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