Phytochemical investigation of the extract of different organs of Silene conoidea. L. using gas chromatography-mass spectrometer in Sabzevar
Subject Areas : Environmental ecology
Keywords: Bioactive, Phytochemicals, Chloroform Fraction, Dichloromethane fraction, Silene weed ,
Abstract :
Silene weed plant (Silene conoidea. L) is from from the Caryophyllacea family and its leaf is medicinal and edible. This plant in traditional and modern medicine, is used for treatment of skin infections and blood coagulation and its medicinal properties are attributed to phenolic compounds. In this research, flower, stem, leaf, and root of S. conoidea was collected from Sabzevar in Spring 2022 and their the chemical compositions of chloroform and dichloromethane fractions were reported by GC-MS. In order to prepare fractions of all the organs under the same conditions, petroleum ether was used for defatting, and chloroform and dichloromethane were used to extract the effective phytochemicals through maceration method. GC-MS analysis of chloroform fractions showed that terpene derivatives, phenolic derivatives, hydrocarbons, fatty acids, heterocyclic derivatives, phenylpropanes, silane derivatives and steroids are the main constituent compounds in all organs. Hydrocarbon derivatives (69.16%) in flower, terpene derivatives (40.46%) in stem, and fatty acid derivatives (40.46% and 63.98%) respectively in leaf and root are the most constituent compounds in chloroform fractions. Also, the GC-MS analysis of dichloromethane fractions showed that terpene derivatives (47.62% and 40.53%) in leaf and stem, respectively, and silane derivatives (52.70%) in root are the most constituent compounds. The properties of solvents play an important role in determining the extraction of phytochemical compounds with unique biological properties. In general, the results of this research showed that the non-polar extract of chloroform and the stem of the plant have valuable effective substances, especially terpene compounds, for medicinal purposes. These results suggested that other genetic resources of S. conoidea can be further explored to screen high bioactive compounds and purification of phytochemical compounds which are valuable to produce, expand, and develop natural antioxidants in the production of bio-medicine and food.
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