Callus induction and essential oil composition of Dracocephalum moldaviaca L.

Subject Areas : Environmental ecology

Syed Mehdi  Razavi, ¹ (1Associate Professor, Department of Biology, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran)
Ali Reza  Ghasemian ² (Assistant Professor, Department of Biology, Faculty of Science, University of Mohaghegh Ardabili,)
Sonia  Mosavi, ³ (3M.Sc student, Department of Biology, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran)
Homa  Arshneshin ⁴ (M.Sc student, Department of Biology, Faculty of Science, University of Mohaghegh Ardabili,)

Received: 2015-11-28 Accepted : 2016-08-15 Published : 2016-09-01

Keywords: Essential oil, Callus, thymol, Dracocephalum moldavica L,

Abstract :

Dracocephalum moldavica L. is an annual aromatic herb, belongs to Lamiaceae family and indigenous of central Asia. In the recent study, we focused on induction of callus tissue and identification of callus essential oil composition. After sterling with ethanol 70% and sodium hypochloride (1%), seeds of the plants were cultured in a Petri dishes line with MS medium. Seedling cotyledons were isolated and transferred to Petri dishes contained MS medium with different combinations of plant hormones, BAP and NAA. For callus formation, the Petri dishes incubated in a growth chamber at 25°C and certain photoperiod of 16/8h light/darkness with 2400 lux light intensity. The produced callus weight was measured for all of treatments for determining optimal hormonal combination. Essential oil compositions of the callus were obtained from the optimal combination by hydro distillation method (Clevenger type apparatus) and were analyzed by gas chromatography-mass spectrometry (GC-MS). The results were showed that callus formation from cotyledons of Dracocephalum moldavica L. seedlings took place in a dose dependent manner of phyto hormones. The maximum Cali genesis was seen in MS medium supplemented with NAA (4 mg/l) and BAP (1.5 mg/l) and thymol (45.99%), decane (26.29%), hexa decanoic acid (7.08%) and dodecane (7.03%) were the main components of callus essential oil.  

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