The effect of ascorbic acid and proline on the germination indices of Aquilegia columbine seeds under salt stress

Subject Areas : Plant physiology

Mehdi Nafar ^{1 *}

1 -

Received: 2024-10-21 Accepted : 2024-12-01 Published : 2025-01-07

Keywords: Aquilegia columbine, Ascorbic Acid, Proline, Salinity Stress,

Abstract :

In order to investigate the effect of ascorbic acid and proline on the germination indices of Aquilegia columbine seeds under salt stress conditions, the present research was conducted as a factorial experiment based on a completely randomized statistical design with three replications. The experimental treatments included ascorbic acid and proline each with concentrations of zero, 50 and 100 mg/liter and salinity stress caused by sodium chloride salt with levels of zero, 50 and 100 mg/liter.  The evaluated traits included germination indices (percentage of germination, radicle length, shoot length), proline amount and catalase and peroxidase enzymes activity. The results showed that the highest percentage of germination, radicle and shoot length were obtained in proline 100 mg/l in the absence of salt treatment. The highest proline amount was in proline 100 mg/L in sodium chloride salinity stress 100 mg/L treatment. The highest catalase and peroxidase enzymes activities were obtained in 100 mg/l ascorbic acid in the absence of salt stress treatment. Therefore, according to the results of the research, it is possible to recommend the use of proline 100 mg/liter under salinity stress conditions caused by sodium chloride salt to improve germination indicators (germination percentage, radicle length, shoot length) and enzyme activity of Aquilegia columbine plant seeds.

 

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