Investigating the Effect of Wheat Germ Oil Toxicity on Breast (MCF7) and Cervical (Hela) Cancer Cell Lines

Subject Areas : Plant physiology

navabh zamani daropi ^{1 *} , Farkhondeh Nemati ² , عباسعلی دهپورجویباری ³ , Roya Bishehkolaei ⁴

1 -
2 - Department of Biology, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran
3 - استاد
4 - Assistant Professor of department of Biology, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran

Received: 2024-09-27 Accepted : 2024-11-16 Published : 2025-01-07

Keywords: MTT test, Wheat germ, MCF7 and Hela cell lines, Medicinal plant,

Abstract :

Cancer is the most important cause of death in the world. Natural products extracted from medicinal plants can play an important role in cancer treatment. Therefore, this study aims to investigate the effect of different concentrations of wheat germ oil on MCF7 and Hela cancer cells. After the cultivation of MCF7 and Hela cell lines, the cells were placed in the vicinity of different concentrations of wheat germ oil (14.65 to 30000 μg/ml) and incubated for 24, 48, and 72 hours. MTT colorimetric test was used to determine cytotoxicity. The results showed that wheat germ in different concentrations significantly reduced cell growth compared to the control group (p˂0.05). The effect of wheat germ oil on cell growth inhibition was dependent on concentration and time, and the highest percentage of cell growth inhibition at the concentration of 30,000 μg/ml was related to MCF7 and Hela cell lines (78.40 and 95.96%, respectively), which was obtained in 72 hours. The hour was obtained. Furthermore, the values of IC50 were calculated as 13148.1 and 10204.5 μg/ml for MCF7 and Hela cells, respectively. The results suggest that different concentrations of wheat germ have cytotoxicity against MCF7 and Hela cell lines. Therefore, more research should be done to find the basic mechanisms of this activity.

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