Investigating the effect of the number of lateral buds, type of barberry shrub and BAP hormone on the growth rate of phenotypic traits of barberry through tissue culture

Subject Areas : Biotechnology

Ghanbar Laei ^{1 *}

1 - Member of Faculty of Agriculture Department, Islamic Azad University, Damghan Branch, Damghan, Iran.

Received: 2024-08-10 Accepted : 2024-09-28 Published : 2024-07-09

Keywords: Tissue culture, ornamental, bud number, BAP hormone, barberry,

Abstract :

In order to investigate the effect of the number of lateral buds, type of barberry shrub and BAP hormone on the growth of phenotypic traits of barberry through tissue culture, a factorial experiment in the form of a completely randomized design in three replications and in three replications. Factors, the first factor of three types of barberry shrubs (seedless, seeded and ornamental), the second factor of BAP hormone at four levels (0, 1, 2, 3 mg/L) and the third factor of the number of lateral buds. It was done at two levels (1 and 2 buds) in the laboratory of Islamic Azad University, Damghan branch in 2018. The culture medium used was based on Mora Shigi and Skoog (MS). After preparing the culture medium and sterilizing it by autoclave, it was distributed in test tubes. After preparation, the samples were cultured in a sterile environment and then the samples were stimulated to grow using growth hormone. The studied traits included the time of greening, number of leaves, length, width and surface of leaves, ratio of width to length of leaves, fresh weight and dry weight of leaves, ratio of fresh to dry weight and freshness after 30 days of cultivation. Finally, after collecting information, the data obtained from the experiment was organized and then variance analysis was performed with SAS statistical software and comparison of averages was performed with Duncan's test. Pearson's correlation coefficient was used to determine the relationship between traits. The triple interaction effect was significant at the 1% probability level on all studied traits except for freshness. Correlation coefficient between the dry weight of leaves with the number of leaves (r=0.86**), leaf length (r=0.78**), leaf width (r=0.73**), leaf surface (*/76 r=0) showed. *) Positive leaf fresh weight (r=0.95**) was significant at 1% probability level.