Study of the antioxidant capacity of silver nanoparticles synthesized from Laurencia caspica seaweed on liver cancer cell line (HepG2)
Subject Areas : Biotechnological Journal of Environmental Microbiology
Shahryar Tagheipour Kouhbane
1
*
,
Mahboubeh Radmehr
2
1 - Researchers and Elites Club, Lahijan Branch, Islamic Azad University, Lahijan, Iran
2 - Department of Geology, Lahijan Branch, Islamic Azad University, Lahijan, Iran.
Keywords: Antioxidant, Silver nanoparticles, Algae extract, Laurencia caspica, Liver,
Abstract :
Introduction: Hepatocele carcinoma is a type of liver cancer that occurs most often after a person has been infected with viral hepatitis B and C or cirrhosis, which is most often due to alcohol consumption.
Objective and importance of the research: Today, finding natural compounds from marine organisms, especially algae, as well as nanoparticles that have antibacterial, antiviral and anticancer effects is of particular importance around the world. The aim of this study was to investigate the antioxidant capacity of silver nanoparticles synthesized with Laurencia caspica seaweed on liver cancer cell line (HepG2).
Research method: In this experimental (interventional) study, HepG2 cell lines were cultured in RPMI1640 medium supplemented with 10% fetal calf serum, 2 mM glutamine, 110 u/m penicillin, 100 μg/ml, 0.1 mM non-essential amino acids and 1 mM sodium pyruvate. After collecting L. caspica samples from the southern coast of the Caspian Sea, the hydroalcoholic extract of the algae was obtained by percolation using 50% methanol solvent. The cells were exposed to silver nanoparticles and L. caspica algae extract at 24-hour intervals.
Results: This study showed that the combination of silver nanoparticles in L. caspica algal extract has a significant effect on the reduction of liver cancer cell line (HepG2). With increasing concentrations of the combination of silver nanoparticles with the extract, significant anticancer effects were observed in the studied combination.
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