Isolation and characterization of antimicrobial activities of native actinomycete strains from agricultural soils in Guilan province
Subject Areas : Biotechnological Journal of Environmental Microbiology
Elham Amiri
1
(Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Guilan, Iran)
Mirsassan Mirpour
2
(Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Guilan, Iran)
Khosro Essazadeh
3
(Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Guilan, Iran)
Behnam Rasti
4
(Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Guilan, Iran)
Keywords: Actinomycetes, Antimicrobial activity, 16S rRNA, Amycolatopsis, Streptomyces,
Abstract :
Actinomycetes are known as the largest reservoir of natural antibiotics in the world. For this reason, due to their ability to produce various antibiotics and other compounds of therapeutic importance, they are considered the golden microorganisms of the 21st century. The purpose of this research is the isolation and molecular identification of actinomycetes with antimicrobial properties from agricultural soils in the native areas of Guilan province. Soil samples were collected from the southwestern agricultural areas of Guilan province. Serial dilution was used to isolate actinomycetes. Then the morphological, physiological, and biochemical identification of the samples was done and finally, the molecular identification of the isolates was done using 16S rRNA sequencing and phylogenetic analysis. Antimicrobial activity was investigated against pathogenic microorganisms. A total of 14 isolates were identified.2 isolates with more antimicrobial properties were selected. Based on the results of phylogenetic studies and 16S rRNA sequencing, Amycolatopsis roodepoortensis strain EA7 with 99.63% confidence, and Streptomyces microflaveus strain EA6 with 93.92% confidence were identified. The isolated bacteria had more antimicrobial activity against Gram-positive pathogenic microorganisms Staphylococcus aureus and standard sample Staphylococcus aureus PTCC 1112. This research is the first report on the identification of actinomycetes with antimicrobial properties in the agricultural soils of the southwestern regions of Guilan province located in the Alborz mountains. The identification of the rare strain of Amycolatopsis roodepoortensis strain EA7 from the northern regions of Iran makes the soils of these regions very valuable.
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